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Mouse Tail SuperDirect PCR Cov Khoom Siv Direct PCR Lysis Reagent (Mouse Tail) (rau Genotyping)
Ncaj nraim siv nas tail lysate ua tus qauv rau PCR amplification, tsis muaj cais rho tawm ntawm DNA, ceev thiab siab rhiab heev.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
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Tsiaj Tissue Direct PCR cov khoom siv-UNG (tsis suav cov cuab yeej siv) raws tu qauv
Ncaj nraim siv cov ntaub so ntswg lysate ua tus qauv rau PCR amplification, tsis muaj cais cais DNA, ceev thiab siab rhiab heev.Ntxiv UNG los tiv thaiv kev ua qias tuaj kom tshem tawm cov khoom cuav.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
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Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping)
Ncaj nraim siv nas tail lysate ua tus qauv rau PCR amplification, tsis muaj cais rho tawm ntawm DNA, ceev thiab siab rhiab heev.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
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Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping)
Tus nas tail lysate yog siv ncaj qha los ua tus qauv rau PCR amplification yam tsis muaj cais tawm ntawm DNA, uas yog ceev thiab rhiab heev.Ntxiv UNG los tiv thaiv kev ua qias tuaj kom tshem tawm cov khoom cuav.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
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Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (zebrafish)
Ncaj nraim siv zebrafish lysate ua tus qauv rau PCR amplification, tsis muaj cais rho tawm ntawm DNA, ceev thiab siab rhiab heev.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
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Cog nplooj Direct PCR Plus cov khoom siv (tsis suav cov cuab yeej) raws tu qauv Direct PCR los ntawm cov khoom cog
Ncaj nraim siv polysaccharide thiab polyphenol nplooj lysate ua tus qauv rau PCR amplification, tsis muaj cais extraction ntawm DNA, ceev thiab siab rhiab heev.
◮Tsis siv sijhawm ntev thiab kim DNA purification (Lub Sijhawm, kev ua haujlwm thiab nqi txuag!)
◮Cov khoom siv tsawg dua
◮Yooj yim-cov qauv tuaj yeem raug rau PCR cov tshuaj tiv thaiv lossis cov tshuaj tiv thaiv lysis yam tsis tau txiav lossis sib tsoo
◮2 X Mix—txo cov qauv thauj khoom yuam kev thiab cov tshuaj tiv thaiv lub sijhawm npaj
◮Fast-template npaj tuaj yeem ua tiav hauv 10 feeb, PCR cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 50 feeb
◮High throughput-lysis cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 96-zoo PCR phaj
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Plant Seed Direct PCR Kits I/II (tsis muaj cov cuab yeej coj mus kuaj) Protocol Plant Direct PCR Kits
◮Tsis siv sijhawm ntev thiab kim DNA purification (Lub Sijhawm, kev ua haujlwm thiab nqi txuag!)
◮Cov khoom siv tsawg dua
◮Yooj yim-cov qauv tuaj yeem raug rau PCR cov tshuaj tiv thaiv lossis cov tshuaj tiv thaiv lysis yam tsis tau txiav lossis sib tsoo
◮2 X Mix—txo cov qauv thauj khoom yuam kev thiab cov tshuaj tiv thaiv lub sijhawm npaj
◮Fast-template npaj tuaj yeem ua tiav hauv 10 feeb, PCR cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 50 feeb
◮High throughput-lysis cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 96-zoo PCR phaj
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Cog nplooj Direct PCR ntxiv rau cov khoom siv-UNG (tsis muaj cov cuab yeej kuaj) raws tu qauv Direct PCR los ntawm cov khoom cog
Ncaj nraim siv polysaccharide thiab polyphenol nplooj lysate ua tus qauv rau PCR amplification, tsis muaj cais extraction ntawm DNA, ceev thiab siab rhiab heev.UNG tiv thaiv kev ua qias tuaj tau ntxiv los tiv thaiv qhov tsis zoo.
◮Tsis siv sijhawm ntev thiab kim DNA purification (Lub Sijhawm, kev ua haujlwm thiab nqi txuag!)
◮Cov khoom siv tsawg dua
◮Yooj yim-cov qauv tuaj yeem raug rau PCR cov tshuaj tiv thaiv lossis cov tshuaj tiv thaiv lysis yam tsis tau txiav lossis sib tsoo
◮2 X Mix—txo cov qauv thauj khoom yuam kev thiab cov tshuaj tiv thaiv lub sijhawm npaj
◮Fast-template npaj tuaj yeem ua tiav hauv 10 feeb, PCR cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 50 feeb
◮High throughput-lysis cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 96-zoo PCR phaj
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RT-qPCR Easyᵀᴹ (Ib kauj ruam)-Taqman
- Ib-Cov khoom siv kauj ruam ua kom rov qab hloov pauv thiab PCR yuav tsum tau nqa tawm hauv tib lub raj, tsuas yog xav tau ntxiv cov qauv RNA, tshwj xeeb PCR primers thiab RNase-Free ddH2O.
- Kev txheeb xyuas ntau npaum li lub sijhawm ntawm tus kab mob RNA lossis kab RNA tuaj yeem ua tiav sai thiab raug.
- Cov khoom siv siv qhov tshwj xeeb Foregene thim rov qab reagent thiab Foregene HotStar Taq DNA Polymerase ua ke nrog cov tshuaj tiv thaiv tshwj xeeb txhawm rau txhim kho kev ua kom zoo dua qub thiab qhov tshwj xeeb ntawm cov tshuaj tiv thaiv.
- Cov tshuaj tiv thaiv zoo tshaj plaws ua rau cov tshuaj tiv thaiv muaj kev paub ntau dua, muaj zog thermal stability, thiab kam rau siab dua.
- RT-qPCR Ib qho yooj yimTM(Ib kauj ruam)-Taqman cov khoom siv los nrog ROX sab hauv siv dye, uas tuaj yeem siv los tshem tawm cov teeb liab keeb kwm yav dhau thiab cov teeb liab tsis raug ntawm qhov dej, uas yooj yim rau cov neeg siv kawg siv hauv cov qauv sib txawv ntawm cov khoom siv PCR ntau.
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RNAlater (Rau RNA Stabilization) RNAlater Stabilization Solution
Kev kho sai sai ntawm cov ntaub so ntswg coj los tshiab, inhibit RNase kev ua, tiv thaiv RNA los ntawm degradation.
-Reagents yog siv ncaj qha, ib kauj ruam yog nyob rau hauv qhov chaw, thiab RNA yog tam sim ntawd stabilized thiab tiv thaiv.
-Kev ua haujlwm ntawm chav sov, yooj yim, nyab xeeb thiab tsis muaj tshuaj lom.
-Txhawm rau tshem tawm cov teeb meem ntawm cov dej khov qhuav los yog lub tub yees txias txias rau kev khaws cov ntaub so ntswg.
-Cov ntaub so ntswg tuaj yeem khaws cia rau lub sijhawm ntev yam tsis muaj kev pheej hmoo ntawm RNA degradation, ua kom ntseeg tau cov ntaub ntawv ntawm cov noob caj noob ces.
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PCR Hero yog ib qho tseem ceeb tshaj plaws ntawm kev ua haujlwm ntawm lub cev
◮Siab fidelity: 6lub sij hawm uas zoo tib yam Taq enzyme;
◮Ceev dua amplification ceev:lub amplification ceev yog 5-10sec / kb, uas yog 6-12 lub sij hawm ntawm zoo tib yam Taq enzyme;
◮Ntau template adaptability: nws muaj peev xwm ua tau zoo amplify ntau yam nyuaj DNA qauv nrog siab GC cov ntsiab lus thiab nyuaj rau amplify;
◮Siab duaAmplification efficiency: tus naj npawb ntawm amplification cycles qis dua li ntawm Taq enzyme zoo tib yam;
◮Smuaj zog Environmental kam rau ua: muab tso rau ntawm 37 ° C rau ib lub lim tiam, tswj ntau tshaj90%kev ua si;
◮Nws muaj 5'→ 3' DNA polymerase kev ua haujlwm thiab 5'→ 3' exonuclease kev ua, tsis muaj 3'→ 5' exonuclease kev ua.
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PCR Ib qho yooj yim thiab yooj yim rau nruab
Cov tshuaj tiv thaiv tshwj xeeb thiab kev ua haujlwm siab Taq DNA Polymerase ua rau PCR cov tshuaj tiv thaiv muaj kev ua kom zoo dua qub, tshwj xeeb thiab rhiab heev.
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