Direct PCR yog dab tsi

Direct PCR yog cov txheej txheem ntawm DNA amplification ncaj qha los ntawm cov ntshav, tsiaj cov ntaub so ntswg, nas tail, cell, nas pob ntseg, zebra ntses, ntses qe, nplooj nplooj, noob los yog lwm yam thawj biological ntaub so ntswg qauv yam tsis ua DNA cais thiab purification cov kauj ruam.Cov txheej txheem PCR ncaj qha tuaj yeem txo lub sijhawm sim, thiab tus nqi hauv genotyping thiab ntau qhov project.Nws kuj tseem muab cov kev xaiv zoo dua thaum ntsib cov teeb meem ntawm kev nthuav dav me me ntawm cov qauv uas cov kauj ruam ua kom huv tuaj yeem ua rau cov qauv poob.

FOREGENE's PCR Mix muaj zog tiv thaiv kev ntxhov siab, vim FOREGENE lub zog patented Taq enzyme (tso cai patent: ZL20161034512.0 (Tuam Tshoj), EP3450560 (Tebchaws Europe), PCT/CN2017/082154 (US), patent 68Jap49).Nws Taq enzyme siv DNA recombination thev naus laus zis los ua kom cov DNA khi cov qauv hauv cheeb tsam ntawm archaeal nucleic acid binding protein nrog cov tshav kub-resistant Taq DNA polymerase los tsim cov qauv DNA nrog kev sib txuas ntxiv.Ib qho kev txhim kho kub-pib Taq uas khaws ntau yam haujlwm ntawm thawj DNA polymerase.This Taq enzyme tuaj yeem khi rau cov qauv DNA thiab pib DNA synthesis hauv cov qauv qis qis thiab cov cheeb tsam nyuaj.

Piv txwv li, nws yooj yim npaum li kev ntxiv koj lub xov tooj ntawm tes, tsob nroj (cov nplooj hluas, cov hauv paus hniav lossis cov noob) lossis cov qauv tsiaj rau qhov tsis, thiab ntxiv cov tswv yim sib xyaw nrog cov primers tshwj xeeb hauv PCR hlab thiab khiav nws los ntawm thermal cycler.Cov khoom siv tau zoo yog qhov zoo tagnrho rau cov qauv ntsuas loj uas txuag lub sijhawm tseem ceeb heev.

PCR cov tshuaj tiv thaiv uas muaj UNG enzyme thiab dUTP raug xaiv raws li cov kev xav tau ntawm kev sim, uas tuaj yeem zam tau cov pa phem los ntawm PCR amplification khoom.