Cov nplooj ntoo cog nplooj ncaj qha PCR cov khoom siv-UNG

Cov nplooj ntoo cog nplooj ncaj qha PCR cov khoom siv-UNG

Cov Khoom Siv Qhia:

Raws cov khoom siv cog nplooj ncaj qha PCR cov khoom siv, dUTP yog siv hloov ntawm dTTP, thiab UNG enzyme uas tuaj yeem thuam cov qauv uas muaj dUTP ntxiv. Nyob rau hauv txoj kev no, qhov tshwj xeeb thiab qhov tseeb ntawm amplification yog ua kom ntseeg tau, thiab cov teeb meem ntawm PCR cov khoom sib kis uas yuav tshwm sim thaum kuaj cov caj ces loj.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv Cheebtsam

Nqe I

Ntiag ntawv P1

Tsis P2

6×DNA Chaw Tos Tsheb

Ntu II

2× Nplooj PCR Ib qho yooj yimTM Sib tov (UNG)

Nta & qhov zoo

■ Tsis muaj sijhawm siv thiab kim DNA ua kom huv
Material Cov khoom siv tsawg dua
■ Yooj yim-cov qauv tuaj yeem raug rau PCR cov tshuaj tiv thaiv lossis lysis cov tshuaj tiv thaiv tsis muaj txiav lossis sib tsoo
■ 2 × sib xyaw, txo qhov piv txwv thauj khoom yuam kev thiab cov kab ke npaj tos lub sijhawm
Preparation Kev npaj nrawm nrawm tuaj yeem ua tiav hauv 10 feeb, PCR cov tshuaj tiv thaiv tuaj yeem ua tiav hauv tsawg kawg 50 feeb
Through High throughput-lysis cov tshuaj tiv thaiv tuaj yeem ua tiav hauv 96-zoo PCR phaj

Khoom siv tsis

Daim Ntawv Thov: cim qhia txog kev hloov kho, kev faib tawm hauv lub cev, thiab lwm yam.
Qauv: cog nplooj
Koob tshuaj: txoj kab uas hla 2-3 hli (txoj kev ncaj), txoj kab uas hla 5-7 hli (txoj kev lysis)
Nrhiav kom tau thaj tsam: phiaj xwm tawg ≤1kb

Ua haujlwm ntws

Plant Leaf Direct PCR Kit-UNG4

Electropherogram

Plant Leaf Direct PCR Kit-UNG5

Ob lub lim tiam ntawm PCR Mix kuaj pom cov kev tshwm sim nyob rau hauv qhov xwm txheej ntawm PCR amplification cov khoom sib kis.


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa rau peb