Ntshav SuperDirectᵀᴹ PCR Kit (UNG)-EDTA Ntshav Direct PCR Master Mix rau Genotyping ntshav
Kit Description:
Ncaj nraim siv EDTA anticoagulated tag nrho cov ntshav ua tus qauv rau PCR amplification, nrog siab rhiab heev thiab siab amplification efficiency.UNG tiv thaiv kev ua qias tuaj tau ntxiv los tiv thaiv qhov tsis zoo.
◮Tsis tas yuav siv sij hawm ntev thiab kim DNA purification, tsis muaj pretreatment, thiab ncaj qha siv tag nrho cov ntshav raws li ib tug PCR template.
◮Lub kaw lus muaj peev xwm ua kom muaj zog thiab tuaj yeem ntes cov genome thiab txawv teb chaws lub hom phiaj DNA tawg hauv cov ntshav.
◮2 × SuperEasyTMMix (UNG)-EDTA muaj ntshav siab: txog li 45% hauv tib neeg cov ntshav thiab 20% hauv cov ntshav nas.
◮Cov qauv no tau kaw tag nrho, yog li tsis tas yuav txhawj xeeb txog cov qauv sib kis thiab cov txiaj ntsig tsis zoo PCR.Anti-pollution PCR system
◮2 × SuperEasyTMMix (UNG)-EDTA, tshem tawm cov pa phem los ntawm PCR cov khoom, thiab xyuas kom meej qhov tseeb thiab qhov tseeb ntawm amplification.
Specifications
200 × 20μl rxns, 2000 × 20μl rxns
2 × SuperEasyTMMix(UNG)-EDTA:Rau lub hauv paus ntawm 2 × SuperEasyTMMix-EDTA, dUTP yog siv los hloov dTTP, thiab UNG enzyme uas tuaj yeem degrade tus qauv uas muaj dUTP ntxiv rau tib lub sijhawm, uas tuaj yeem tiv thaiv kev sib kis ntawm PCR amplification khoom.Muaj qhov tshwj xeeb hloov kho D-Taq DNA Polymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis zoo, PCR cov tshuaj tiv thaiv zoo dua, kev ua kom zoo dua qub thiab ruaj khov, thiab lwm yam.2O rau qhov sib thooj 2 × SuperEasyTMMix (UNG)-EDTA siv rau hauv cov tshuaj tiv thaiv PCR.
6 × DNA Loading Buffer: Lub Chaw Thauj Khoom Tsis Muaj SDS.Nws raug nquahu kom siv 6 × DNA Loading Buffer nrog rau cov khoom siv thaum ua haujlwm agarose gel electrophoresis kom tau txais txiaj ntsig zoo electrophoresis.Tsis txhob siv Loading Buffer uas muaj SDS, txwv tsis pub yuav muaj tus Tsov tus tw loj ntawm lub teeb ci hauv txoj kab thaum lub sij hawm electrophoresis, uas yuav cuam tshuam rau qhov kev sim.
Cov khoom siv
| 2 × SuperEasyTMMix (UNG)-EDTA |
| 6 × DNA Loading Buffer |
| Cov lus qhia |
Nta & qhov zoo
- Tsis tas yuav siv sijhawm ntev thiab kim DNA purification, tsis muaj pretreatment, thiab ncaj qha siv tag nrho cov ntshav raws li PCR template.
-Lub kaw lus muaj peev xwm ua kom muaj zog thiab tuaj yeem ntes cov genome thiab txawv teb chaws lub hom phiaj DNA tawg hauv cov ntshav.
-2 × SuperEasyTMMix (UNG)-EDTA muaj ntshav siab: txog li 45% hauv tib neeg cov ntshav thiab 20% hauv cov ntshav nas.
-Cov qauv raug kaw tag nrho, yog li tsis tas yuav txhawj xeeb txog cov qauv sib kis thiab cov txiaj ntsig tsis zoo PCR.Anti-pollution PCR system 2 × SuperEasyTMMix (UNG)-EDTA, tshem tawm cov pa phem los ntawm PCR cov khoom, thiab xyuas kom meej qhov tseeb thiab qhov tseeb ntawm amplification.
Daim ntawv thov Kit
Dedicated rau ncaj qha PCR txheeb xyuas ntawm EDTA anticoagulated tag nrho cov ntshav.(xws li: tib neeg ntshav, ntshav nas, ntshav qaib, ntshav noog, ntshav nyuj, ntshav dev, thiab lwm yam)
Kev ua haujlwm
Cia thiab Txee lub neej
Cov khoom siv no khaws cia ntawm -20 ° C.
2 × SuperEasyTMMix (UNG)-EDTA, Yog tias siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ° C rau lub sijhawm luv luv (siv li ntawm 10 hnub).
6 × DNA Loading Buffer, Nws tuaj yeem khaws cia ntawm 4 ° C lossis -20 ° C rau lub sijhawm ntev.
