ForeDirect RT-qPCR cov khoom siv
Kit Description:
◮Rau RNA ncaj qha mus rau lub sij hawm amplification los ntawm swab collections tsis muaj RNA purification txheej txheem.Cov khoom siv no ua kom tiav qRT-PCR cycles hauv ib teev.Cov enzyme mix yog ib qho kev ua kom zoo ntawm Reverse Transcriptase, Kub-Start Taq DNA polymerase, RNase inhibitor.Lub Reaction Buffer muaj tag nrho cov khoom uas yuav tsum tau muaj, suav nrog cov khoom siv tsis zoo, Mg2+, dUTP, thiab dNTPs.
Kev piav qhia
ForeDirect RT-qPCR Cov Khoom Siv yog tsim los rau RNA ncaj qha lub sijhawm nthuav dav los ntawm kev sib sau swab yam tsis muaj RNA purification txheej txheem.Cov khoom siv no ua kom tiav qRT-PCR cycles hauv ib teev.Cov enzyme mix yog ib qho kev ua kom zoo ntawm Reverse Transcriptase, Kub-Start Taq DNA polymerase, RNase inhibitor.Lub Reaction Buffer muaj tag nrho cov khoom uas yuav tsum tau muaj, suav nrog cov khoom siv tsis zoo, Mg2+, dUTP, thiab dNTPs.
Specifications
100 T
Cov khoom siv
| Nucleic acid tso tawm tus neeg sawv cev |
| RNA tiv thaiv |
| 2 × RT-qPCR Tsis |
| Enzyme Mixture (Taq & M-MLV) |
| Cov lus qhia |
Kev thov PCR
1. Thaum PCR cov tshuaj tiv thaiv cov raj tau luv luv centrifuged, muab tso rau hauv cov qauv tank ntawm amplification ntsuas.
2. Thermal raws tu qauv
| Kauj ruam | Kub | Sijhawm | Lub voj voog | |
| 1 | Rov qab transcription | 50 ℃ | 15 feeb | 1 |
| 2 | Pre-denaturation | 95 ℃ | 1 min | 1 |
| 3 | Denaturation | 95 ℃ | 10 vib nas this | 42 |
| 4 | Anneal/Extension | 60 ℃ | 30 vib nas this | |
Nco tseg:Fluorescent teeb liab tau kuaj pom tam sim tom qab qhov txuas ntxiv ntawm txhua lub voj voog.
3. Tom qab teeb tsa, txuag cov ntaub ntawv thiab khiav qhov kev pabcuam tshuaj tiv thaiv.
Cov kauj ruam ua haujlwm
Ua ntej reagent npaj, tag nrho cov reagents nyob rau hauv cov khoom yuav tsum tau thawed nyob rau hauv chav tsev kub thiab sib tov maj mam.
Kev npaj reagent
1. Npaj cov Nucleic Acid Release Mix
| Cheebtsam | Volume per Sample lossis Control |
| Nucleic acid tso tawm tus neeg sawv cev | 5l miv |
| RNA tiv thaiv | 0,5l ua |
2. Npaj Kev Sib Tw Sib Nqus
| Cheebtsam | Volume per Sample lossis Control |
| 2 × RT-qPCR Tsis | 15 mL |
| Enzyme Mixture (Taq & M-MLV) | 1.5 l ua |
| Primers thiab Probes | 1 m l |
Cia thiab Txee lub neej
-Cov khoom siv yuav tsum khaws cia ntawm -20± 5℃.Lub sijhawm siv tau yog 12 lub hlis.
-Tsis txhob rov khov-thaw cycles (<5 cycles).
