Direct RT-qPCR cov khoom siv
Kit Description:
◮ Direct RT-qPCR Cov Khoom Siv, siv Foreasy Reverse Transcriptase thiab Foreasy HS Taq DNA Polymerase tsim los ntawm Foregene, nrog cov tshuaj tiv thaiv tsis zoo, ua rau cov khoom siv no muaj zog tiv thaiv thiab ua tau zoo, thiab nws tuaj yeem kuaj cov tshuaj tiv thaiv ncaj qha siv Foregene Lysis system ua qauv.Cov khoom siv no tuaj yeem thov rau COVID-19 cov khoom siv kuaj pom nucleic acid thiab lwm yam kab mob kab mob nucleic acid nrhiav cov khoom tsim kho.
Kev piav qhia
Direct RT-qPCR Cov Khoom Siv muab cais ntim RT-qPCR tsis muaj thiab muaj txiaj ntsig zoo enzymesib tov (Taq, M-MLV, RI), ua kom yooj yim rau kev tsim cov khoom siv rov qab thiab qhov systemkev hloov kho, hauv kev txheeb xyuas yooj yim.
Specifications
500T, 50,000T
Cov khoom siv
| Cov khoom siv (20 μL cov tshuaj tiv thaiv kab mob) | IM-05111-01 ib | IM-05112-01 ib |
| 500 T | 50,000 T | |
| Direct Enzyme Mixture | 500 mL× 1 | 50 ml ib× 1 |
| 2 × Direct RT-qPCR Buffer | 1 ml ib× 5 | 500 ml× 1 |
| Kev qhia | 1 | 1 |
Cov kauj ruam ua haujlwm
Ua ntej reagent npaj, tag nrho cov reagents nyob rau hauv cov khoom yuav tsum tau thawed nyob rau hauv chav tsev kub thiab sib tov maj mam.
A: Npaj of tus qauv thiab reagent
1. Npaj npaj RNA template (Nws raug nquahu kom siv Foregene Total RNA Isolation Kit series cov khoom siv los rho tawm thiab ua kom huv RNA template) lossis cov khoom lag luam tawg (Nws pom zoo kom siv Foregene Lysis system), cov primers tshwj xeeb (10 μM) thiab lwm yam khoom siv, ntsuas.
2. Muab 2 × Direct RT-qPCR Buffer, RNase-Free ddH2O thiab 20 × ROX Reference Dye (yog tias nws's xav tau) nyob rau hauv lub thawv nrog dej khov, ua cov no yaj lawm, thiab sib tov maj mam.
B: Npaj of RT-qPCR qhov system
Rooj 1 : Npaj of RT-qPCR ssystem
| Cheebtsam | Ntim | Thaum kawg concentration |
| 2 × Direct RT-qPCR Buffer | 10 mL | 1 × |
| Direct Enzyme Mixture | 1μL | |
| Forward Primer (10 μM) | 0,8l ua | 50-900 nM |
| Rov qab Primer (10 μM) | 0,8l ua | 50-900 nM |
| Kev sojntsuam (4 μM) | 1μL | 200 nM |
| Template (RNA los yog Lysate) | X μL | |
| 20 × ROX Reference Dye | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Tag nrho ntim | 20μL |
C: Teeb tus RT-QPER tshuaj tiv thaiv qhov kev pab cuam
Rooj 2 : RT-qPCR reaction qhov kev pab cuam kev teeb tsa (rau piv txwv: ob-kauj ruam txoj kev)
| Kauj ruam | Kub | Sijhawm | Lub voj voog | Cov ntsiab lus | |
| 1 | 50 ℃ | 15 feeb | 1* | 1 | Rov qab transcription |
| 2 | 95 ℃ | 1 min | 1 | Pre-denaturation | |
| 3 | 95 ℃ | 10 sec | 40-45 : kuv | Denaturation | |
| 60 ℃ | 30 sec 2 * | Anneal/Extension | |||
Cia thiab Txee lub neej
-Cov khoom siv yuav tsum khaws cia ntawm -20± 5℃.Lub sijhawm siv tau yog 12 lub hlis.
-Tsis txhob rov khov-thaw cycles (<5 cycles).
