"Control tus qauv los ntawm cov ntsiab lus, qhia lub zog los ntawm qhov zoo".Peb lub lag luam tau siv zog los tsim kom muaj ib pab neeg ua haujlwm tau zoo thiab ruaj khov thiab tshawb nrhiav cov txheej txheem kev tswj xyuas zoo rau Kev Pabcuam OEM qPCR reagents-Taqman, Peb yuav txhawb nqa tib neeg los ntawm kev sib txuas lus thiab mloog, teeb tsa ua piv txwv rau lwm tus thiab kawm los ntawm kev paub dhau los.
"Control tus qauv los ntawm cov ntsiab lus, qhia lub zog los ntawm qhov zoo".Peb lub lag luam tau siv zog los tsim kom muaj ib pab neeg ua haujlwm tau zoo thiab ruaj khov thiab tshawb nrhiav cov txheej txheem tswj kev ua haujlwm zoo rauTuam Tshoj Isolation Kit thiab Test Kit, Peb pawg kws tshaj lij engineering feem ntau yuav npaj los pab koj rau kev sib tham thiab tawm tswv yim.Peb tseem tuaj yeem muab cov qauv dawb rau koj kom tau raws li koj qhov yuav tsum tau ua.Kev siv zog zoo tshaj plaws yuav raug tsim los muab kev pabcuam zoo tshaj plaws rau koj thiab cov khoom muag.Thaum koj txaus siab rau peb cov lag luam thiab cov khoom, xyuas kom koj hais lus rau peb los ntawm kev xa email rau peb lossis hu rau peb sai.Hauv kev sib zog kom paub peb cov khoom muag thiab tuam txhab ntxiv, koj tuaj yeem tuaj rau peb lub Hoobkas los saib nws.Peb yuav feem ntau txais tos qhua los ntawm thoob plaws lub ntiaj teb no rau peb lub lag luam los tsim kev lag luam kev sib raug zoo nrog peb.Nco ntsoov xav tias tsis muaj nqi los hais lus rau peb rau kev lag luam me thiab peb ntseeg tias peb yuav qhia txog kev ua lag luam zoo tshaj plaws rau tag nrho peb cov tub lag luam.

Cov lus piav qhia

2X tiag PCR Yooj yim^TMMix-Taqman muab los ntawm Real Time PCR Easy^TM-Taqman cov khoom siv yog ib qho tshiab premix system uas siv cov fluorescent probes rau Real Time PCR amplification cov tshuaj tiv thaiv, uas tuaj yeem txhim kho cov khoom tshwj xeeb thiab cov tshuaj tiv thaiv rhiab heev.ROX yog muab raws li kev tswj dye.

2X tiag PCR Yooj yim^TMMix-Taqman muaj Foregene qhov tshwj xeeb kub pib Taq DNA Polymerase.Piv nrog rau cov enzymes Taq zoo tib yam, nws muaj qhov zoo ntawm kev ua haujlwm siab amplification, muaj zog tshwj xeeb amplification muaj peev xwm thiab tus nqi tsis sib xws.Nws tuaj yeem txo qhov tsis muaj qhov tshwj xeeb amplification thiab txhim kho qhov tseeb ntawm PCR.

Specifications

Nta & qhov zoo

■ Yooj yim—2X PCR Mix kom txo tau qhov kev sim ua yuam kev thiab lub sijhawm ua haujlwm

■ Tshwj xeeb - optimized tsis thiab kub-pib Taq enzyme tuaj yeem tiv thaiv tsis muaj qhov tshwj xeeb amplification thiab primer dimer tsim

■ Muaj qhov rhiab heev— tuaj yeem tshawb pom cov ntawv luam tsawg ntawm cov qauv

■ Muaj ntau yam zoo-sib haum nrog feem ntau cov khoom siv PCR hauv lub sijhawm

Daim ntawv thov Kit

QPCR tsom xam

Kev khiav haujlwm

Duab

Cia thiab txee lub neej

Cov khoom siv no yuav tsum muab khaws cia kom deb ntawm lub teeb thiab yuav tsum khaws cia ntawm -20 ℃.Yog tias siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm luv luv (10 hnub).

"Control tus qauv los ntawm cov ntsiab lus, qhia lub zog los ntawm qhov zoo".Peb lub lag luam tau siv zog los tsim ib pab neeg ua haujlwm tau zoo thiab ruaj khov thiab tshawb nrhiav cov txheej txheem kev tswj xyuas zoo rau Kev Pabcuam Kev Pabcuam OEM qPCR reagents-Taqman, Peb yuav txhawb nqa tib neeg los ntawm kev sib txuas lus thiab mloog, teeb tsa ua piv txwv rau lwm tus thiab kawm los ntawm kev paub dhau los.
Muab OEMTuam Tshoj Isolation Kit thiab Test Kit, Peb pawg kws tshaj lij engineering feem ntau yuav npaj los pab koj rau kev sib tham thiab tawm tswv yim.Peb tseem tuaj yeem muab cov qauv dawb rau koj kom tau raws li koj qhov yuav tsum tau ua.Kev siv zog zoo tshaj plaws yuav raug tsim los muab kev pabcuam zoo tshaj plaws rau koj thiab cov khoom muag.Thaum koj txaus siab rau peb cov lag luam thiab cov khoom, xyuas kom koj hais lus rau peb los ntawm kev xa email rau peb lossis hu rau peb sai.Hauv kev sib zog kom paub peb cov khoom muag thiab tuam txhab ntxiv, koj tuaj yeem tuaj rau peb lub Hoobkas los saib nws.Peb yuav feem ntau txais tos qhua los ntawm thoob plaws lub ntiaj teb no rau peb lub lag luam los tsim kev lag luam kev sib raug zoo nrog peb.Nco ntsoov xav tias tsis muaj nqi los hais lus rau peb rau kev lag luam me thiab peb ntseeg tias peb yuav qhia txog kev ua lag luam zoo tshaj plaws rau tag nrho peb cov tub lag luam.

Tsis muaj amplification signals

1.Cov Taq DNA Polymerase nyob rau hauv cov khoom siv poob nws cov kev ua si vim yog qhov khoom siv tsis raug los yog tas sij hawm ntawm cov khoom siv.
Kev pom zoo: Paub meej tias qhov chaw cia khoom ntawm cov khoom siv;rov ntxiv qhov tsim nyog ntawm Taq DNA Polymerase rau PCR system lossis yuav ib lub Sijhawm PCR tshiab rau cov kev sim cuam tshuam.

2.Muaj ntau cov inhibitors ntawm Taq DNA Polymerase hauv DNA template.
Tswv yim: Rov kho tus qauv los yog txo tus nqi ntawm cov qauv siv.

3.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR Mix peb muab yog 3.5mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.

4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.

5.Tus qauv ntawm tus qauv yog tsawg lossis ntau dhau.
Kev pom zoo: Ua cov qauv linearization gradient dilution, thiab xaiv cov template concentration nrog qhov zoo tshaj plaws PCR nyhuv rau Real Time PCR sim.

NTC muaj tus nqi fluorescence siab dhau lawm

1.Reagent paug tshwm sim thaum lub sijhawm ua haujlwm.
Kev pom zoo: Hloov nrog cov tshuaj reagents tshiab rau kev sim PCR tiag tiag.

2.Contamination tshwm sim thaum lub sij hawm npaj ntawm cov tshuaj tiv thaiv PCR.
Kev pom zoo: Siv cov kev tiv thaiv tsim nyog thaum lub sijhawm ua haujlwm, xws li: hnav cov hnab looj tes latex, siv lub taub hau nrog lub lim dej, thiab lwm yam.

3.Cov primers yog degraded, thiab qhov degradation ntawm cov primers yuav ua rau tsis yog tshwj xeeb amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.

Primer dimer lossis tsis tshwj xeeb amplification

1.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR EasyTM Mix peb muab yog 3.5 mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.

2.Lub PCR annealing kub yog tsawg dhau.
Tswv yim: Ua kom lub PCR annealing kub los ntawm 1 ℃ lossis 2 ℃ txhua zaus.

3.Cov khoom PCR ntev dhau lawm.
Kev pom zoo: Qhov ntev ntawm cov khoom PCR tiag tiag yuav tsum nyob nruab nrab ntawm 100-150bp, tsis ntau tshaj 500bp.

4.Cov primers yog degraded, thiab degradation ntawm cov primers yuav ua rau cov tsos ntawm tej yam amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.

5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.

Tsis zoo repeatability ntawm quantitative values

1.Cov cuab yeej ua haujlwm tsis zoo.
Cov lus qhia: Tej zaum yuav muaj qhov yuam kev ntawm txhua lub PCR qhov ntawm lub ntsuas, uas ua rau tsis zoo rov tsim dua thaum tswj qhov kub thiab txias.Thov xyuas raws li cov lus qhia ntawm cov cuab yeej sib txuas.

2.Cov qauv purity tsis zoo.
Kev pom zoo: Cov qauv tsis huv yuav ua rau qhov tsis zoo ntawm qhov kev sim, uas suav nrog cov purity ntawm cov qauv thiab cov primers.Nws yog qhov zoo tshaj plaws los ntxuav cov qauv, thiab cov primers yog qhov zoo tshaj plaws purified los ntawm SDS-PAGE.

3.Qhov kev npaj PCR thiab lub sijhawm khaws cia ntev dhau lawm.
Cov lus qhia: Siv lub sijhawm tiag tiag PCR system rau PCR sim tam sim tom qab kev npaj, thiab tsis txhob tso nws tseg ntev dhau.

4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.

5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.