SARS-CoV-2 Variant Nucleic Acid Detection Kit II (Multiplex PCR Fluorescent Probe Method)-rau kev kuaj xyuas cov variants los ntawm UK, South Africa, Brazil, thiab India
Kit Description:
◮ Direct PCR:Tsis xav tau cov khoom siv nucleic acid extaction thiab System, ua tiav 96 tus qauv hauv 1 teev lossis li ntawd.
◮ Cov khoom siv tsawg tsawg thiab siv tau yooj yim heev:Tsuas yog xav tau Real-Time PCR system.
◮ LoD thiab siab rhiab heev:Kev soj ntsuam rhiab heev tuaj yeem ncav cuag qib qis tshaj li 500 daim ntawv / ml.
Specifications
48 Rxns/kit, 96 Rxns/kit
| Tsis muaj. | Cheebtsam | Tus nqi | Cov khoom tseem ceeb | |||
| 48r xws | 96r xws | |||||
| 1 | Nucleic acid tso tawm tus neeg sawv cev | 1.4 mL / tub | 2 cev | 5.3 mL / lub raj mis | 1 fwj | Surfactant |
| 2 | RNA tiv thaiv | 53 μL / tub | 1 cev | 106 μL / tub | 1 cev | RNase inhibitor |
| 3 | SARS-CoV-2 Variant tshuaj tiv thaiv A | 800 μL / tub | 1 cev | 1600 μL / tub | 1 cev | Primer, sojntsuam, cov tshuaj tiv thaiv tsis zoo, dNTP |
| 4 | SARS-CoV-2 Variant tshuaj tiv thaiv B | 800 μL / tub | 1 cev | 1600 μL / tub | 1 cev | Primer, sojntsuam, cov tshuaj tiv thaiv tsis zoo, dNTP |
| 5 | SARS-CoV-2 enzyme mix | 160 μL / tub | 1 cev | 320 μL / tub | 1 cev | Kub pib Taq enzyme, M-MLV enzyme |
| 6 | SARS-CoV-2 kev tswj zoo | 100 μL / tub | 1 cev | 100 μL / tub | 1 cev | Recombinant plasmid muaj cov phiaj xwm tawg, RNA |
| 7 | SARS-CoV-2 negative control | 1200 μL / tub | 1 cev | 1200 μL / tub | 1 cev | TE tsis |
Lus Cim: Cov khoom siv los ntawm cov khoom siv sib txawv tsis tuaj yeem siv sib hloov.
Nta & qhov zoo:
Ceev thiab yooj yim: ua kom tiav qhov kev xeem hauv li 1 teev
Tsis txhob poob nucleic acid: tsis xav tau nucleic acid purification
Tsawg nrhiav kom pom kev txwv thiab siab rhiab heev, tshwj xeeb tshaj yog suitabe rau loj-scale nrhiav kom tau sai
Cov khoom siv tsawg tsawg thiab siv tau yooj yim heev
Kev kuaj pom rhiab heev tuaj yeem ncav cuag qib qis tshaj li 500 daim ntawv / mL).
Yuav siv li cas:
1.Cov cuab yeej siv tau
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
2.Txoj kev kuaj xyuas
Qauv ua: Naopharyngeal lossis oropharyngeal swab qauv (tsuas yog 1 kauj ruam ua haujlwm)(Tos li 10 feeb)
PCR amplificaton (<= 55 feeb)
Kev ua haujlwm:
Cia:
kaw los ntawm lub teeb thiab khaws cia ntawm -20 ± 5 ℃;
Khaws ntawm 2-8 ℃ tom qab qhib, siv hauv 7 hnub;zam rov qab khov-thaw (<5 zaug)
Lub neej txee:1 xyoo
