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Plaub Lub Ntsiab Tseem Ceeb Rau PCR Cov Khoom Siv Hluav Taws Xob Tswj
1: Hloov cov khoom siv sim hauv lub sijhawm teeb tsa (NTC) tsis zoo tswj thiab rov ua nws ntau zaus.Thaum pom tias muaj PCR cov khoom paug hauv chav kuaj, hloov tag nrho cov khoom siv sim hauv lub sijhawm.Xws li: rov dilute thiab npaj cov primers, re-sterilize lub pipette taub, E ...Nyeem ntxiv -
Ob lub luag haujlwm RT-PCR enzymes
Ib txwm rov qab transcriptases tsis tuaj yeem tiv taus qhov kub thiab txias (qhov kub zoo rau kev ua haujlwm MMLV yog 37-50 ° C, thiab AMV yog 42-60 ° C).Cov kab mob sib kis ntau dua RNA tsis tuaj yeem thim rov qab tau zoo rau hauv cDNA ntawm qhov kub thiab txias, ua rau kev tshawb pom kev ua haujlwm txo qis.Tra...Nyeem ntxiv -
Nucleic acid isothermal amplification technology
PCR yog qhov siv ntau tshaj nucleic acid amplification technology thiab yog dav siv vim nws rhiab heev thiab tshwj xeeb.Txawm li cas los xij, PCR yuav tsum tau rov ua cov thermal denaturation thiab tsis tuaj yeem tshem tawm cov kev txwv ntawm kev cia siab rau cov cuab yeej thiab cov cuab yeej siv, uas txwv nws daim ntawv thov hauv kev kho mob ...Nyeem ntxiv -
Qhov tshuaj tiv thaiv mRNA yog dab tsi?
Qhov tshuaj tiv thaiv mRNA yog dab tsi Cov tshuaj tiv thaiv mRNA hloov RNA mus rau cov hlwb ntawm lub cev los qhia thiab tsim cov protein antigens tom qab kev hloov kho hauv vitro, yog li ua rau lub cev tsim cov tshuaj tiv thaiv kab mob tiv thaiv antigen, yog li nthuav cov bod ...Nyeem ntxiv -
PCR Technology
PCR (polymerase saw cov tshuaj tiv thaiv) yog ib qho ntawm in-vitro DNA amplification technologies, nrog rau keeb kwm ntawm ntau tshaj 30 xyoo.PCR thev naus laus zis tau pioneer los ntawm Kary Mullis ntawm Cetus, Tebchaws Asmeskas hauv xyoo 1983. Mullis tau thov rau PCR patent xyoo 1985 thiab luam tawm thawj daim ntawv qhia txog PCR ntawm Science hauv tib lub ...Nyeem ntxiv -
Kev kuaj pom tus mob coronavirus tshiab, los ntawm nucleic acid, tshuaj tiv thaiv kab mob rau antigen, yuav ua li cas xaiv qhov normalization ntawm kev sib kis?
Ib qho kev sib kis tau hloov lub ntiaj teb.Thoob plaws ntiaj teb, tsoomfwv ntawm txhua lub tebchaws tab tom ntsib cov teeb meem loj hauv kev tiv thaiv thiab tswj kev kis tus kabmob.Thaum lub sijhawm COVID-19 kis thoob qhov txhia chaw, Tuam Tshoj yog nyob rau plaub theem ntawm kev tiv thaiv thiab teb lub hauv paus (kev tiv thaiv, nrhiav pom, tswj thiab Tus yuam sij rau su...Nyeem ntxiv -
Plaub txoj hauv kev tiv thaiv PCR cov khoom sib kis
Tsim SOP System Tsim PCR kev sim SOP los ua tus qauv coj tus cwj pwm ntawm cov neeg ua haujlwm sim.Cov neeg sim ua raws li cov txheej txheem ua haujlwm, thiab txo qis PCR cov pa phem uas yuav tshwm sim los ntawm tib neeg los yog tiv thaiv qhov tshwm sim ntawm cov pa phem hauv kev ua haujlwm.Hauv addi...Nyeem ntxiv -
Yuav ua li cas txhim kho qhov ua tau zoo ntawm LncRNA thim rov qab?
Lead Long non-coding RNA, lncRNA yog ib qho tsis-coding RNA nrog ntev dua 200 nucleotides, feem ntau ntawm 200-100000 nt.lncRNA tswj cov noob qhia ntawm epigenetic, transcription thiab post-transcriptional theem, thiab koom nrog X chromosome silencing, genome imprinting thiab chroma ...Nyeem ntxiv -
Nws tuaj yeem kis los ntawm ib tus neeg mus rau lwm tus hauv 14 vib nas this.Yuav ua li cas tshwm sim rau "kev sib kis heev" Delta strain hauv Zhong Nanshan Pass?
Tranaslated qhov chaw: WuXi AppTec pab neeg editor Hauv Guangzhou, Suav teb, tub ceev xwm lub luag haujlwm pabcuam kev tshawb nrhiav kabmob kis tau tshaj tawm cov vis dis aus saib: Hauv tib lub tsev noj mov, ob leeg tau taug kev mus rau hauv chav dej ib tom qab tsis muaj kev sib cuag ntawm lub cev.Tsuas yog 14 vib nas this ntawm co-e ...Nyeem ntxiv -
SNP molecular labeling thiab kuaj pom
Tom qab tus kws tshawb fawb Asmeskas Eric S. Lander tau tsim tawm ib qho nucleotide polymorphism (SNP) ua tus cim thib peb hauv xyoo 1996, SNP tau siv dav hauv kev txheeb xyuas kev lag luam kev lag luam, kev siv tshuaj lom neeg txuas nrog daim ntawv qhia, thiab kev tshuaj xyuas tib neeg kab mob., D...Nyeem ntxiv -
Nrov Science |Yuav kuaj tus mob coronavirus li cas los ntawm qPCR
COVID-19 yog ib yam kab mob sib kis los ntawm tus mob hnyav ua pa nyuaj rau tus mob Coronavirus Hom 2. Thaum tus neeg kis tus kab mob, cov tsos mob tshwm sim feem ntau suav nrog kub taub hau, hnoos, thiab ua tsis taus pa.Cov qauv siv los kuaj tau tuaj yeem sau los ntawm nasopharyngeal swabs lossis oropharyngeal swabs.Qhov kuv...Nyeem ntxiv -
Puas yog koj tseem siv thev naus laus zis tsim tau 30 xyoo dhau los los rho tawm RNA?
Puas yog koj tseem siv Trizol los rho RNA?Gosh, tsis txhob cia kuv txhawj txog koj txoj kev noj qab haus huv!Trizol tab tom yuav raug marginalized, tsis txhob qhia kuv tias koj tseem nyob hauv qhov tsaus ntuj?Lub ntsiab tseem ceeb ntawm Trizol reagent yog phenol, thiab cov organic reagents xws li chloroform kuj tau ntxiv rau hauv qhov kev sim ...Nyeem ntxiv
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