• facebook
  • linkedin
  • youtube

xov xwm 716 (1)

Puas yog koj tseem siv Trizol los rho RNA?
Gosh, tsis txhob cia kuv txhawj txog koj txoj kev noj qab haus huv!
Trizol tab tom yuav raug marginalized, tsis txhob qhia kuv tias koj tseem nyob hauv qhov tsaus ntuj?

Lub ntsiab tseem ceeb ntawm Trizol reagent yog phenol, thiab cov organic reagents xws li chloroform kuj tau ntxiv rau hauv kev sim ua haujlwm.Kev kis mus ntev yuav ua rau muaj kev phom sij rau koj txoj kev noj qab haus huv, tshwj xeeb tshaj yog kev sib cuag ntawm daim tawv nqaij tuaj yeem ua rau muaj tshuaj lom neeg kub hnyiab thiab nti mus tas li.

Raws li ib txwm muaj, cov neeg koom tes hauv chav kuaj tsuas tuaj yeem txo qhov kev phom sij los ntawm kev ua kom tag nrho lawv tus kheej (hnav khaub ncaws, hnab looj tes thiab qhov ncauj qhov ntswg).

Thiab kuv cov lus qhia rau koj yog: thov hloov Trizol ncaj qha kom tsis txhob raug mob los ntawm qhov chaw.

1. Thawj tiam khoom ntawm RNA extraction
Tawm hauv xyoo 1987 (Trizol reagents)

qhov zoo:
Ntxiv tiav extraction

Qhov tsis zoo:
Cov tshuaj toxicity xws li guanidine isothiocyanate-phenol-chloroform yuav tsum tau;

Kev ua haujlwm nyuaj;
Yuav tsum siv DNase kom tshem tawm cov kab mob DNA

2.Qhov thib ob tiam khoom ntawm RNA extraction
Tuaj tawm xyoo 1994 (QIAGEN RNeasy thiab cov khoom siv hauv tsev feem ntau)

qhov zoo:
Kem centrifugal txoj kev yog saws, uas yooj yim rau kev khiav lag luam;

Qhov tsis zoo:
Yuav tsum tau centrifuge kom tshem tawm cov ntaub so ntswg

Yuav tsum siv DNase kom tshem tawm cov kab mob DNA

3.Qhov thib peb tiam khoom ntawm RNA extraction
Tawm hauv xyoo 2005 (QIAGEN RNeasy Plus thiab Fcov oregeneRNA purification series khoom)

qhov zoo:
Siv ncaj qha rau DNA tshem tawm kem kom tshem tawm DNA thiab cov ntaub so ntswg pov tseg tib lub sijhawm, txuag lub sijhawm thiab kev siv zog;

Muaj kev nyab xeeb thiab yooj yim, tsis tas yuav ntxiv lim kab thiab DNase kom yooj yim tshem tawm impurities thiab zam DNA paug

xov xwm 716 (2)

Cia peb saib qhov kev ua haujlwm sib txawv ntawm thawj tiam khoom (Trizol), cov khoom thib ob (RNeasy) thiab cov khoom thib peb (Foregene) ntawm RNA extraction:xov 716 (3)

Kev ua haujlwm hauv daim duab yog qhov tseeb: cov khoom lag luam thib peb muaj qhov pom tseeb zoo

Tsuas yog peb lo lus los piav qhia: yooj yim!Ceev ceev!Kev nyab xeeb

Daim ntawv qhia PK ntawm cov txiaj ntsig kev simrauthawj tiam, thib ob thiab tiam thib peb cov khoom ntawm RNA extraction:

1. Eelectrophoretogram

Thawj tiam (1: Trizol)
The second generation (2:QIAN RNeasy)
Peb tiam (3: QIAGEN RNeasy ntxiv, 4: Foregene)

xov 716 (4)

Yam khoom zoo li cas, koj yuav paub los ntawm kev sib piv
RNA rho tawm siv cov khoom siv Foregene
Tsis tsuas yog siab concentration tab sis kuj zoo kev ncaj ncees

2.qPCR graph (amplification nkhaus graph)

xov 716 (5)
Thawj tiam (Trizol)

xov 716 (6)

Tiam ob (sky bluetxhais tau tiasQIAGEN RNeasy-, liabtxhais tau tiasQIAGEN RNeasy +)

xov xwm 716 (7)

Peb tiam (xiav txhais tau tias Foregene, ntsuab txhais tau tias QIAGEN RNeasy ntxiv)

(Ceeb Toom: QIAGEN RNeasy thib ob ntawm qPCR daim duab txhais tau hais tias cov khoom siv tsis ntxiv DNase thiab tsis tshem cov kab mob DNA. Yog li ntawd, CT siab dua thiab qhov tshwm sim tsis raug; QIAGEN RNeasy + txhais tau tias DNase tau ntxiv thiab cov DNA kis tau raug tshem tawm, thiab qhov tshwm sim yog qhov tseeb)

Txawm hais tias nws yog electrophoretogram lossis ntau PCR amplification curve graph, qhov zoo ntawm Foregene cov khoom tsis yog qis dua rau cov khoom txawv teb chaws.


Post lub sij hawm: Lub Xya hli ntuj-16-2021