Tsim SOP System

Tsim kev sim PCR SOP los ua tus qauv coj tus cwj pwm ntawm cov neeg ua haujlwm sim.

Cov neeg sim ua raws li cov txheej txheem ua haujlwm, thiab txo qis PCR cov pa phem uas yuav tshwm sim los ntawm tib neeg los yog tiv thaiv qhov tshwm sim ntawm cov pa phem hauv kev ua haujlwm.Tsis tas li ntawd, tus kws sim yuav tsum muaj kev paub txog kev txawj ntse thiab kev txawj ntse, suav nrog kev paub txog kev ua haujlwm ntawm cov cuab yeej siv, qhia meej txog tag nrho cov txheej txheem ua haujlwm, paub txog cov txheej txheem kev kho mob ntawm cov kab mob sib kis thiab cov txheej txheem kuaj xyuas kom zoo, thiab tuaj yeem txhais cov txiaj ntsig kev xeem kom raug.

Tsev Standard PCR Laboratory

Lub PCR lub chaw soj nstuam tau muab faib ua plaub qhov hauv cov hauv paus ntsiab lus, uas yog thaj chaw npaj reagent, thaj chaw ua piv txwv, thaj chaw amplification, thiab thaj chaw tsom xam cov khoom lag luam.Thawj ob thaj chaw yog thaj chaw ua ntej amplification, thiab ob qhov kawg yog thaj chaw tom qab amplification.Lub pre-amplification cheeb tsam thiab tom qab amplification cheeb tsam yuav tsum tau nruj me ntsis sib cais.Cov ntaub ntawv sim, reagents, sau ntawv, cwjmem, cov ntaub ntawv tu, thiab lwm yam tuaj yeem tsuas yog ntws los ntawm thaj chaw ua ntej mus rau thaj tsam tom qab kev nthuav dav, uas yog, los ntawm thaj chaw reagent npaj, qhov chaw ua piv txwv, thaj chaw amplification, thiab cov khoom lag luam amplification cheeb tsam, thiab yuav tsum tsis txhob ntws rov qab.Lub airflow nyob rau hauv lub chav kuaj yuav tsum kuj ntws los ntawm lub pre-amplification cheeb tsam mus rau lub post-amplification cheeb tsam, tsis nyob rau hauv lub rov qab kev taw qhia.

Txo cov kauj ruam sim

Yog tias lub chaw kuaj xyuas tsuas yog ua PCR tshawb pom thiab txheeb xyuas, nws raug nquahu kom siv fluorescent quantitative PCR es tsis txhob siv PCR.

Fluorescence quantitative PCR nrhiav tau cov txiaj ntsig tuaj yeem sau thiab txheeb xyuas los ntawm cov teeb liab fluorescent, yog li tsis tas yuav qhib lub hau rau electrophoresis tom qab cov tshuaj tiv thaiv, uas zam kev kis kab mob ntawm PCR cov khoom tshwm sim los ntawm kev xau ntawm cov khoom siv tshuaj tiv thaiv los ua aerosols.Yog tias koj nce tus naj npawb ntawm lub hau qhib thaum lub sij hawm thauj khoom ntawm gel electrophoresis, aerosol paug yuav tshwm sim.Nws raug pom zoo kom txhawb nqa daim ntawv thov ntawm PCR ntau thiab maj mam hloov cov PCR zoo.

Txais yuav UNG tiv thaiv kev ua qias tuaj

Lub UNG anti-PCR cov khoom kis kab mob yog siv rau cov tshuaj tiv thaiv PCR.

Lub kaw lus siv dUTP hloov dTTP.Tom qab cov tshuaj tiv thaiv PCR, tag nrho cov khoom PCR (DNA fragments) tau koom nrog dUTP;Nyob rau hauv ib puag ncig tom ntej ntawm PCR cov tshuaj tiv thaiv, UNG enzyme ntxiv rau hauv lub cev yog incubated ntawm 37 ° C rau 5 feeb ua ntej PCR, uas tuaj yeem yog qhov tshwj xeeb Degrade tag nrho cov DNA seem uas muaj dUTP, thiab tom qab ntawd ua PCR cov tshuaj tiv thaiv.Qhov no tuaj yeem tshem tawm cov kab mob aerosol los ntawm PCR cov khoom.Cov nyhuv yog pom nyob rau hauv daim duab hauv qab no:

Lus Cim: Rau cov PCR ncaj qha, koj tuaj yeem xaiv cov khoom lag luam ntawm cov khoom lag luam tiv thaiv PCR ntawm FJ Biotech

Qhia

Rau cov chaw soj nstuam uas ua qhov loj-loj genotyping kuaj, nws tau pom zoo kom siv UNG anti-PCR cov khoom kis kab mob rau kev sim tshuaj reagents ntxiv rau kev tsim kho cov chaw sim tsim nyog.

Ceeb Toom: Kev siv cov kab ke no tsis tuaj yeem tshem tawm PCR cov khoom sib kis uas twb tau tshwm sim lawm.Yog li ntawd, UNG system yuav tsum tau siv thaum pib ntawm qhov kev xeem cuam tshuam, thiab UNG system yuav tsum ib txwm siv rau PCR amplification, thiaj li tiv thaiv kev kis kab mob ntawm cov khoom PCR.False positive.

Nws raug pom zoo kom siv Direct PCR-UNG system ntawm Forge Biotech thaum ua qhov kev sim loj, xws li: Plant Leaf Direct PCR Kit-UNG;

Plant Seed Direct PCR Kit-UNG;

Tsiaj Tissue Direct PCR Kit-UNG;

Mouse Tail Direct PCR Cov Khoom Siv-UNG;

Zebra Ntses Direct PCR Kit-UNG.

Cov khoom siv no los ntawm Foregene tuaj yeem tsis tsuas yog ua kom pom PCR sai thiab loj, tab sis kuj tseem tiv thaiv thiab tswj cov khoom sib kis ntawm PCR.