Peb ib txwm xav thiab xyaum coj raws li qhov kev hloov ntawm qhov xwm txheej, thiab loj hlob tuaj.We purpose at the achievement of a richer mind and body as well as the living for Wholesale Dealers of Professional Magnetic Viral Rna Nucleic Acid Isolation Purification Prepacked Kits, Peb lub tuam txhab ua haujlwm nrog cov txheej txheem hauv paus ntsiab lus ntawm "kev ncaj ncees-raws li, kev koom tes tsim, tib neeg oriented, yeej-yeej kev koom tes".Peb cia siab tias peb tuaj yeem yooj yim muaj kev sib koom tes zoo nrog cov neeg ua lag luam los ntawm thoob plaws ib puag ncig.
Peb ib txwm xav thiab xyaum coj raws li qhov kev hloov ntawm qhov xwm txheej, thiab loj hlob tuaj.Peb lub hom phiaj ntawm kev ua tiav ntawm lub siab thiab lub cev nplua nuj nrog rau kev ua neej nyobTuam Tshoj Universal Rna DNA Reagents thiab Tsis Siv Neeg Nucleic Acid Purification, Peb xav ua kom tau raws li qhov xav tau ntawm peb cov neeg siv khoom thoob ntiaj teb.Peb cov khoom lag luam thiab cov kev pabcuam yog txuas mus ntxiv kom tau raws li cov neeg siv khoom xav tau.Peb zoo siab tos txais cov qhua tuaj noj mov tshiab thiab qub los ntawm txhua qhov chaw ntawm lub neej los tiv tauj peb rau yav tom ntej kev lag luam kev sib raug zoo thiab ua tiav kev sib koom ua ke!
Phau ntawv qhia:

 Peb ib txwm xav thiab xyaum coj raws li qhov kev hloov ntawm qhov xwm txheej, thiab loj hlob tuaj.We purpose at the achievement of a richer mind and body as well as the living for Wholesale Dealers of Professional Magnetic Viral Rna Nucleic Acid Isolation Purification Prepacked Kits, Peb lub tuam txhab ua haujlwm nrog cov txheej txheem hauv paus ntsiab lus ntawm "kev ncaj ncees-raws li, kev koom tes tsim, tib neeg oriented, yeej-yeej kev koom tes".Peb cia siab tias peb tuaj yeem yooj yim muaj kev sib koom tes zoo nrog cov neeg ua lag luam los ntawm thoob plaws ib puag ncig.
Lag luam wholesale Dealers ntawmTuam Tshoj Universal Rna DNA Reagents thiab Tsis Siv Neeg Nucleic Acid Purification, Peb xav ua kom tau raws li qhov xav tau ntawm peb cov neeg siv khoom thoob ntiaj teb.Peb cov khoom lag luam thiab cov kev pabcuam yog txuas mus ntxiv kom tau raws li cov neeg siv khoom xav tau.Peb zoo siab tos txais cov qhua tuaj noj mov tshiab thiab qub los ntawm txhua qhov chaw ntawm lub neej los tiv tauj peb rau yav tom ntej kev lag luam kev sib raug zoo thiab ua tiav kev sib koom ua ke!

Phau ntawv qhia txog teeb meem

Cov nram qab no tsom xam ntawm cov teeb meem koj yuav ntsib nyob rau hauvCog tag nrhoRNA extraction will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

Kab rov tav yog clogged

Kev thaiv ntawm kab rov tav yuav ua rau RNA tawm los txo qis lossis txawm tias tsis tuaj yeem ua kom huv kom tau RNA, thiab qhov zoo ntawm RNA tau txais yuav qis.

Common Cause Analysis:

1. Cov qauv tsis tawg tag.

Cov qauv tsis tiav kev tawg tuaj yeem thaiv DNA-Tshuaj Kem, uas tuaj yeem cuam tshuam rau RNA yield thiab zoo.Peb pom zoo tias thaum ua cov qauv fragmentation, ceev nrooj zom cov kua nitrogen txaus kom tawg cov ntaub so ntswg xws li cov phab ntsa ntawm tes thiab cell membranes ntawm cov qauv kom ntau li ntau tau.Rau cov qauv cog ntawm polyphenol polysaccharides, peb xav kom koj siv Plant Total RNA Isolation Kit Plus.

2.Aspirate lub DNA-Tshuaj Kem cais cov supernatant, aspirate lub cell debris pellet.

Aspirated cell debris pellet tuaj yeem txhaws RNA nkaus xwb Kem thaum lub sij hawm RNA adsorption cov txheej txheem (saib kauj ruam 5 ntawm cov txheej txheem, kauj ruam 6 ntawm cov txheej txheem polysaccharide polyphenol).Peb pom zoo tias yuav tsum tau saib xyuas thaum aspirating no supernatant kom tsis txhob muaj cov khib nyiab ntawm tes raug aspirated.

3. Thawj tus nqi ntawm cov qauv yog loj heev.

Kev siv cov qauv ntau dhau yuav ua rau tsis tiav cov qauv tawg lossis tsis tiav lysis ntawm cov hlwb los ntawm Buffer PRL1 lossis Buffer PSL1, uas ua rau muaj cov kab purification uas txhaws rau kev ua haujlwm huv.Plant Total RNA Isolation Kit muaj qhov pib siab tshaj plaws ntawm 50 mg rau ib zaug purification ntawm ib qho qauv ua haujlwm.Rau cov qauv cog ntawm polyphenol polysaccharides, peb xav kom koj sim cog tag nrho RNA Isolation Kit Plus.

4. Qhov kub ntawm lub centrifuge yog qis heev.

Tag nrho RNA rho tawm thiab ua kom huv tsuas yog ua kua nitrogen cuam tshuam ntawm cov qauv ntaub so ntswg, tag nrho cov kauj ruam yog ua nyob rau hauv chav tsev kub (20-25 ° C).Qee cov centrifuges uas tsis tshua muaj kub muaj qhov kub thiab txias qis dua 20 ° C, uas tuaj yeem ua rau muaj kev cuam tshuam hauv DNA-Tshuaj Kem thiab / lossis RNA nkaus xwb.Yog tias qhov no tshwm sim, teem lub centrifuge kub rau 20-25 ° C thiab ua ntej sov lub lysis sib tov thiab / los yog ntxiv ntawm ethanol sib cais supernatant mus rau 37 ° C.

Tsis muaj RNA rho tawm lossis RNA tawm los tsawg

Feem ntau muaj ntau yam uas cuam tshuam rau kev rov ua haujlwm zoo, xws li: qauv RNA cov ntsiab lus, txoj kev ua haujlwm, qhov ntim elution, thiab lwm yam.

Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim hauv qab no:

1.Ib da dej dej khov lossis kub qis (4 ° C) centrifugation tau ua thaum lub sijhawm ua haujlwm.

Tswv yim: Ua haujlwm ntawm chav tsev kub (15-25 ° C) nyob rau hauv tag nrho cov txheej txheem, tsis txhob ua dej khov da dej thiab tsis kub centrifugation.

       2.RNA tau raug degraded vim yog kev khaws cia tsis raug ntawm cov qauv lossis kev khaws cia ntev ntawm tus qauv.

Cov lus pom zoo: Cov qauv sau tshiab yuav tsum tau khov sai sai hauv cov kua nitrogen, thiab tom qab ntawd khaws cia ntawm -80 ° C rau lub sijhawm ntev, tsis txhob rov khov thiab thawing ntawm cov qauv;lossis tam sim ntawd tsau cov qauv hauv RNA stabilizer RNAlater tov (cov qauv tsiaj txhu).

       3.Tsis txaus cov qauv fragmentation thiab lysis ua rau blockage ntawm purification kem.

Tswv yim: Thaum sib tsoo cov ntaub so ntswg, thov xyuas kom meej tias cov ntaub so ntswg yog av txaus, thiab sai sai hloov mus rau lub pre-npaj Buffer PSL1 (kom paub meej tias qhov tseeb feem ntawm β-ME tau ntxiv, saib cov kauj ruam 1 ntawm cov txheej txheem).

4.Qhov eluent tau ntxiv tsis raug.

Tswv yim: Nco ntsoov tias RNase-Free ddH₂O yog dripped rau hauv nruab nrab ntawm lub purification kem membrane.

5.Qhov tseeb ntim ntawm ethanol tsis tau ntxiv rau Buffer PSL2 lossis Buffer PRW2.

Tswv yim: Thov ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm ethanol rau Buffer PSL2 thiab Buffer PRW2 thiab sib tov zoo ua ntej cov khoom siv.

6.Cov qauv ntawm cov ntaub so ntswg tsis tsim nyog.

Tswv yim: Siv 50 mg ntawm cov ntaub so ntswg rau 500 μl ntawm Buffer PSL1.Kev siv cov ntaub so ntswg ntau dhau yuav txo tau cov RNA rho tawm thiab qhov purity ntawm qhov tshwm sim RNA kuj yuav raug txo.Peb xav kom pom zoo tias thawj zaug koob tshuaj yuav tsum tsis txhob ntau tshaj 50 mg rau RNA rho tawm haujlwm.

7. Tsis tsim nyog elution ntim lossis tsis tiav elution.

Tswv yim: Lub ntim eluent ntawm purification kem yog 50-200 μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom ncua lub sijhawm ntawm chav tsev kub tom qab ntxiv preheated RNase-Free ddH₂O, xws li 5-10 min.

8.Cov purification kem muaj ethanol residue tom qab ntxuav nrog Buffer PRW2.

   Cov lus qhia: Yog tias lub raj khoob yog centrifuged rau 1 min thiab tseem muaj ethanol seem tom qab ntxuav hauv Buffer PRW2, koj tuaj yeem nce lub sij hawm ntawm lub raj khoob centrifugation mus rau 2 min, los yog tso cov purification kem ntawm chav tsev kub rau 5 min kom tshem tawm cov ethanol seem.

9.Cov khoom siv tau siv tsis raug.

   Tswv yim: Rau cov qauv cog ntawm polyphenolic polysaccharides, siv cov khoom siv xws li Plant Total RNA Isolation Kit yuav tsis tuaj yeem tau txais cov qauv RNA zoo tagnrho.Peb pom zoo kom koj siv Plant Total RNA IsolationKit Plus, uas yog tsim tshwj xeeb rau cov qauv cog polyphenolic polysaccharide.Cov khoom siv tshwj xeeb tsim los rau rho tawm RNA los ntawm polyphenol thiab polysaccharide cog cov qauv.

OD260 / OD280 tus nqi qis

RNA elution nrog ddH₂O thiab siv rau kev nyeem ntawv spectrophotometer ua rau qis OD260 / OD280 qhov tseem ceeb.Peb pom zoo kom siv 10 mM Tris-HCl, pH 7.5 (tsis yog RNase-Free ddH)₂O rau elute RNA) kom tau txais qhov tseeb OD260/OD280 qhov tseem ceeb, saib "RNA Concentration thiab Purification Assays" ntawm nplooj 19.

Lub purified RNA yog degraded

Qhov zoo ntawm purified RNA muaj feem xyuam rau yam xws li cov qauv khaws cia, RNase contamination, thiab manipulation.

Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim:

1.Cov ntaub so ntswg tsis tau muab khaws cia rau hauv lub sijhawm tom qab sau.

    Kev pom zoo: Yog tias cov ntaub so ntswg tsis siv rau lub sijhawm tom qab sau, thov khaws cia rau hauv cov kua nitrogen ntawm qhov kub thiab txias tam sim ntawd lossis hloov mus rau -80 ° C rau lub sijhawm ntev cia tom qab ceev ceev hauv cov kua nitrogen, lossis tam sim ntawd tso cov qauv hauv RNA stabilizer RNAlater tov (cov qauv tsiaj txhu).Rau RNA rho tawm, sim siv cov ntaub so ntswg tshiab tshiab.

2.Repeated khov thiab thawing ntawm cov ntaub so ntswg kuaj.

   Cov lus qhia: Thaum khaws cov ntaub so ntswg, nws yog qhov zoo tshaj plaws los txiav rau hauv me me rau kev khaws cia, thiab tshem tawm ib feem ntawm lawv thaum siv lawv kom tsis txhob degradation ntawm RNA los ntawm kev rov ua kom khov thiab thawing ntawm cov qauv.

3.RNase tau qhia hauv chav ua haujlwm lossis tsis hnav cov hnab looj tes, qhov ncauj qhov ntswg, thiab lwm yam.

   Tswv yim: RNA extraction thwmsim yog ua tau zoo tshaj plaws nyob rau hauv cais RNA kev khiav hauj lwm, thiab lub chav kuaj lub rooj yuav tsum tau ntxuav ua ntej qhov kev sim, thiab pov tseg hnab looj tes thiab lub qhov ncauj qhov ntswg yuav tsum tau hnav thaum lub sij hawm sim kom tsis txhob RNA degradation los ntawm kev qhia RNase mus rau qhov loj tshaj plaws.

4.Cov reagent muaj paug los ntawm RNase thaum siv.

   Tswv yim: Hloov nrog ib tug tshiab series ntawm cov nroj tsuag tag nrho RNA extraction kits rau lwm yam thwmsim.

5.Cov centrifuge raj thiab pipette cov lus qhia siv rau RNA manipulation yog kis nrog RNase.

Cov lus qhia: Xyuas kom tseeb tias cov raj centrifuge, cov lus qhia pipette, pipettes, thiab lwm yam siv hauv RNA extraction yog txhua yam RNase-Free.

Phau ntawv qhia:

Plant Total RNA Isolation Kit Instruction Manual