Zoo heev 1st, thiab Client Supreme yog peb cov lus qhia kom xa tus neeg muab kev pabcuam zoo tshaj plaws rau peb lub zeem muag.Tam sim no, peb tau nrhiav peb qhov zoo tshaj plaws los ua ib qho ntawm cov neeg xa khoom zoo tshaj plaws hauv peb cov kev qhuab qhia kom tau raws li cov neeg yuav khoom xav tau ntau dua rau cov neeg siv khoom Bacteria Genomic DNA Extraction Kit-T134H, Raws li ib pab pawg neeg paub txog peb kuj txais tailor.Lub hom phiaj tseem ceeb ntawm peb lub tuam txhab yog ib txwm tsim kom muaj lub cim xeeb txaus siab rau txhua tus neeg yuav khoom, thiab teeb tsa kev sib koom ua lag luam mus ntev-yeej.
Zoo heev 1st, thiab Client Supreme yog peb cov lus qhia kom xa tus neeg muab kev pabcuam zoo tshaj plaws rau peb lub zeem muag.Tam sim no, peb tau nrhiav peb qhov zoo tshaj plaws los ua ib tus neeg xa khoom zoo tshaj plaws hauv peb txoj kev qhuab qhia kom tau raws li cov neeg yuav khoom xav tau ntau dua.Tuam Tshoj Bacteria Genomic DNA Extraction thiab Viral Rna Extraction Kit, Peb muaj ntau xyoo kev paub hauv cov khoom siv plaub hau, thiab peb pawg QC nruj thiab cov neeg ua haujlwm txawj yuav xyuas kom meej tias peb muab koj cov plaub hau kev daws teeb meem nrog cov plaub hau zoo tshaj plaws thiab kev ua haujlwm zoo.Koj yuav tau txais kev lag luam zoo yog tias koj xaiv koom tes nrog cov kws tshaj lij cov chaw tsim khoom.Zoo siab txais tos koj kev koom tes nrog kev txiav txim!
Phau ntawv qhia:

Zoo heev 1st, thiab Client Supreme yog peb cov lus qhia kom xa tus neeg muab kev pabcuam zoo tshaj plaws rau peb lub zeem muag.Tam sim no, peb tau nrhiav peb qhov zoo tshaj plaws los ua ib qho ntawm cov neeg xa khoom zoo tshaj plaws hauv peb cov kev qhuab qhia kom tau raws li cov neeg yuav khoom xav tau ntau dua rau cov neeg siv khoom Bacteria Genomic DNA Extraction Kit-T134H, Raws li ib pab pawg neeg paub txog peb kuj txais tailor.Lub hom phiaj tseem ceeb ntawm peb lub tuam txhab yog ib txwm tsim kom muaj lub cim xeeb txaus siab rau txhua tus neeg yuav khoom, thiab teeb tsa kev sib koom ua lag luam mus ntev-yeej.
Cov khoom ntiag tugTuam Tshoj Bacteria Genomic DNA Extraction thiab Viral Rna Extraction Kit, Peb muaj ntau xyoo kev paub hauv cov khoom siv plaub hau, thiab peb pawg QC nruj thiab cov neeg ua haujlwm txawj yuav xyuas kom meej tias peb muab koj cov plaub hau kev daws teeb meem nrog cov plaub hau zoo tshaj plaws thiab kev ua haujlwm zoo.Koj yuav tau txais kev lag luam zoo yog tias koj xaiv koom tes nrog cov kws tshaj lij cov chaw tsim khoom.Zoo siab txais tos koj kev koom tes nrog kev txiav txim!

Phau ntawv qhia txog teeb meem

The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.

Tsawg tawm los lossis tsis muaj DNA

Feem ntau muaj ntau yam cuam tshuam rau cov txiaj ntsig ntawm genomic DNA, suav nrog qhov chaw ntawm tus qauv, hnub nyoog ntawm tus qauv, qhov chaw cia ntawm cov qauv, thiab kev ua haujlwm.

Genomic DNA tsis tuaj yeem tau txais thaum lub sijhawm rho tawm

1. Cov qauv ntawm cov ntaub so ntswg tsis raug cai los yog khaws cia ntev dhau lawm, ua rau cov DNA genomic degradation.

Kev pom zoo: Khaws cov ntaub so ntswg hauv cov kua nitrogen lossis -20°C;sim siv cov qauv sau tshiab rau kev rho tawm DNA genomic.

2. Cov qauv me me dhau lawm yuav ua rau cov DNA sib raug zoo tsis raug rho tawm.

Tswv yim: Rau cov qauv ntaub so ntswg uas tau muab khaws cia rau ntev los yog muaj cov genomic DNA degradation hnyav, tus nqi ntawm cov ntaub so ntswg tuaj yeem tsim nyog ntxiv txhawm rau txhawm rau rho tawm cov genomic DNA ntau heev.Tus nqi ntawm cov qauv tuaj yeem txiav txim siab raws li DNA xav tau, tab sis cov qauv tshiab yuav tsum tsis pub tshaj 100mg, thiab cov qauv qhuav yuav tsum tsis pub tshaj 30mg.

3. Cov qauv tsis yog hauv av nrog kua nitrogen los yog muab tso rau ntev heev tom qab cov kua nitrogen.

Cov lus qhia: Thaum lub sij hawm DNA rho tawm, cov qauv yuav tsum tau ua kom tiav hauv av nrog cov kua nitrogen kom tawg phab ntsa ntawm tes;Tom qab sib tsoo, thov hloov cov qauv hmoov rau PL1 preheated ntawm 65°C sai li sai tau (thaum cov hmoov av melted, cov genomic DNA yuav pib degrade sai).

4. Kev cia tsis raug ntawm Foregene Protease ua rau txo qis lossis tsis ua haujlwm.

Kev pom zoo: Txheeb xyuas qhov chaw cia ntawm Foregene Protease lossis hloov nws nrog Foregene Protease tshiab rau enzymatic hydrolysis.

5. Cov khoom siv tsis raug cai lossis khaws cia ntev dhau lawm, ua rau qee yam khoom siv tsis raug.

Daim ntawv pom zoo: Yuav cov khoom cog tshiab genomic DNA rho tawm cov khoom siv rau kev ua haujlwm.

6. Kev siv cov khoom siv tsis raug.

Cov Lus Qhia: Yuav Cov Khoom Siv Tsob Ntoo DNA Tso Cai rau cov qauv rau kev rho tawm thiab ntxuav cov nroj tsuag genomic DNA.

7. Buffer WB yam tsis tau ntxiv atsis muaj dej ethanol.

Kev pom zoo: Nco ntsoov ntxiv qhov tseeb ntim ntawm ethanol rau Buffer WB.

8. Lub eluent tsis yog dripped mus rau silica membrane kom raug.

Tswv yim: Ntxiv pre-heated eluent ntawm 65℃dropwise mus rau nruab nrab ntawm cov silica gel daim nyias nyias, thiab cia nws nyob rau hauv chav tsev kub rau 5 feeb los ua kom cov elution efficiency.

Kev rho tawm kom tau txais cov txiaj ntsig qis genomic DNA

1. Cov qauv no raug muab khaws cia tsis raug lossis khaws cia ntev dhau lawm, ua rau muaj qhov degradation ntawm genomic DNA.

Kev pom zoo: Khaws cov ntaub so ntswg ntawm -20℃;sim siv cov ntaub so ntswg tshiab tshiab rau kev rho tawm DNA genomic.

2. Yog tias cov qauv ntawm cov ntaub so ntswg me me dhau, cov genomic DNA rho tawm yuav tsawg dua.

Cov lus qhia: Qee cov qauv cog muaj cov dej nplua nuj, xws li cov nroj tsuag dej xws li algae, thiab lwm yam., qhov ntau npaum li cas tuaj yeem tsim nyog los yog cov dej tuaj yeem dehydrated me ntsis ua ntej ua haujlwm.

3. Cov qauv no tsis huv hauv av nrog cov kua nitrogen los yog raug tso rau hauv chav sov ntev ntev tom qab sib tsoo.

Tswv yim: Cov kua nitrogen sib tsoo yuav tsum txaus, thiab cov qauv ntawm cov phab ntsa yuav tsum tau tawg kom ntau li ntau tau;tam sim ntawd tom qab sib tsoo, cov qauv hmoov yuav tsum raug xa mus rau 65℃preheated Buffer PL1 rau cov kauj ruam tom ntej.

4. Tsis siv cov khoom siv kom raug.

Daim ntawv pom zoo: Siv cov khoom siv cog DNA cais tawm rau kev rho tawm thiab ntxuav cov nroj tsuag genomic DNA.

5. Kev cia tsis raug ntawm Foregene Protease ua rau txo qis lossis tsis ua haujlwm.

Kev pom zoo: Txheeb xyuas qhov chaw cia ntawm Foregene Protease lossis hloov nws nrog Foregene Protease tshiab rau enzymatic hydrolysis.

6. Eluent teeb meem

Kev pom zoo: Thov siv Buffer EB rau elution;yog siv ddH₂O los yog lwm yam eluents, xyuas kom meej pH ntawm eluent nyob nruab nrab ntawm 7.0-8.5.

7. Lub eluent tsis yog dripped kom raug

Tswv yim: Thov ntxiv cov elution nco mus rau nruab nrab ntawm cov silica membrane thiab cia nws nyob rau hauv chav tsev kub li 5 feeb kom nce elution efficiency.

8. Lub ntim eluent tsawg dhau

Tswv yim: Thov siv qhov eluent rau genomic DNA elution raws li cov lus qhia, yam tsawg kawg nkaus tsis pub tsawg tshaj 100ua m l.

Extracted genomic DNA nrog tsawg purity

Qhov qis purity ntawm genomic DNA yuav ua rau tsis ua hauj lwm los yog cov nyhuv tsis zoo ntawm cov kev sim qis qis, xws li: cov enzyme tsis tuaj yeem txiav, thiab lub hom phiaj gene fragment tsis tuaj yeem tau los ntawm PCR.

1. Lwm yam kab mob sib kis, RNA paug.

Kev tshuaj xyuas: Tsis siv neeg PW tsis siv los ntxuav kab;Tsis PW tsis tau siv los ntxuav lub kem ntawm qhov tseeb centrifugation ceev.

Tswv yim: sim xyuas kom meej tias tsis muaj nag lossis daus nyob rau hauv lub supernatant thaum lub supernatant dhau los ntawm kab;Nco ntsoov ntxuav lub purification kem nrog Buffer PW raws li cov lus qhia, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.

2. impurity ion muaj kuab paug.

Kev tshuaj xyuas: Buffer WB ntxuav kem raug tshem tawm lossis tsuas yog ntxuav ib zaug xwb, ua rau muaj cov kab mob ionic seem.

Kev pom zoo: Nco ntsoov ntxuav ob zaug nrog Buffer WB raws li cov lus qhia kom tshem tawm cov ions seem ntau li ntau tau.

3. Cov kab mob RNase.

Kev tsom xam: Exogenous RNase yog ntxiv rau qhov tsis;Kev ntxuav tsis raug hauv Buffer PW yuav ua rau RNase seem thiab cuam tshuam rau cov kev sim RNA hauv qab, xws li hauv vitro transcription.

Cov lus qhia: Foregene series nucleic acid extraction kits tuaj yeem tshem tawm RNA yam tsis muaj RNase ntxiv, thiab tag nrho cov reagents hauv Plant DNA Isolation Kit tsis xav tau RNase;Nco ntsoov ntxuav lub purification kem nrog Buffer PW raws li cov lus qhia, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.

4. Ethanol residues.

Kev tshuaj xyuas: Tom qab ntxuav lub purification kem nrog Buffer WB, tsis muaj lub raj centrifugation tau ua.

Kev pom zoo: Ua raws li cov lus qhia kom tsim nyog khoob raj centrifugation.

Phau ntawv qhia:

Plant DNA Isolation Kit Instruction Manual