Uas muaj tus cwj pwm zoo thiab kev vam meej rau cov neeg siv khoom txaus siab, peb lub koom haum txhim kho peb cov khoom zoo kom ua tau raws li qhov xav tau ntawm cov neeg yuav khoom thiab tseem tsom mus rau kev nyab xeeb, kev ntseeg siab, ib puag ncig tshwj xeeb, thiab kev tsim kho tshiab ntawm Hoobkas Khoom Siv Tuam Tshoj Yooj Yim thiab Ceev Rt-PCR Dspathtm 4X Ib-kauj ruam Multiplex Master Mix, Peb cov neeg ua haujlwm paub txog kev ua haujlwm yuav ua rau koj lub siab dawb paug.Peb zoo siab tos txais koj tuaj yeem nres los ntawm peb lub vev xaib thiab tuam txhab thiab xa koj cov lus nug rau peb.
Uas muaj tus cwj pwm zoo thiab kev vam meej rau cov neeg siv khoom txaus siab, peb lub koom haum tau txhim kho peb cov khoom zoo kom ua tau raws li qhov xav tau ntawm cov neeg yuav khoom thiab tsom mus ntxiv rau kev nyab xeeb, kev ntseeg siab, ib puag ncig specifications, thiab innovation ntawmTuam Tshoj TAQ DNA Polymerase, Qpcr, Peb tam sim no muaj cov kev daws teeb meem zoo tshaj plaws thiab cov kws tshaj lij kev muag khoom thiab kev pab cuam. Nrog rau kev txhim kho peb lub tuam txhab, peb muaj peev xwm muab cov neeg siv khoom zoo tshaj plaws, kev txhawb nqa zoo, kev pabcuam zoo tom qab muag.

Cov lus piav qhia

Cov khoom siv no siv cov txheej txheem lysis tsis zoo uas tuaj yeem tso tawm RNA sai sai los ntawm cov qauv ntawm tes rau RT-qPCR cov tshuaj tiv thaiv, yog li tshem tawm cov sijhawm siv sijhawm thiab siv zog RNA purification txheej txheem.RNA template tuaj yeem tau txais hauv 7 feeb xwb.5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR reagents muab los ntawm cov khoom siv tuaj yeem tau txais cov txiaj ntsig PCR sai thiab muaj txiaj ntsig zoo.

5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR muaj zog inhibitor kam rau ua, thiab lysate ntawm cov qauv siv tau los ua tus qauv rau RT-qPCR ncaj qha.Cov khoom siv no muaj qhov tshwj xeeb RNA high-affinity Foregene reverse transcriptase, thiab Kub D-Taq DNA polymerase, dNTPs, MgCl₂, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.

Specifications

200 × 20μl Rxns, 1000 × 20μl Rxns

Cov khoom siv

Nta & qhov zoo

■ Yooj yim thiab siv tau: nrog Cell Direct RT thev naus laus zis, cov qauv RNA tuaj yeem tau txais hauv 7 feeb xwb.

■ Cov qauv xav tau me me, tsawg li 10 lub hlwb tuaj yeem kuaj tau.

■ Kev xa tawm siab: nws tuaj yeem ntes RNA sai sai hauv cov kab mob hauv 384, 96, 24, 12, 6-zoo daim hlau.

■ DNA Eraser tuaj yeem tshem tawm cov genomes sai sai, txo qhov cuam tshuam rau cov txiaj ntsig kev sim tom ntej.

■ Optimized RT thiab qPCR system ua rau ob-kauj ruam RT-PCR thim rov qab tau zoo dua thiab PCR ntau dua, thiab tiv taus ntau dua rau RT-qPCR cov tshuaj tiv thaiv.

Daim ntawv thov Kit

Scope ntawm daim ntawv thov: kab lis kev cai.

- RNA tso tawm los ntawm cov qauv lysis: tsuas yog siv tau rau RT-qPCR qauv ntawm cov khoom siv no.

- Cov khoom siv tuaj yeem siv rau cov hom phiaj hauv qab no: kev tshuaj ntsuam gene qhia, kev txheeb xyuas ntawm siRNA-mediated gene silencing effect, tshuaj ntsuam xyuas tshuaj, thiab lwm yam.

Daim duab

Cia thiab Txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum khaws cia ntawm 4 ℃;Ntu II yuav tsum khaws cia ntawm -20 ℃.

Foregene Protease Plus II yuav tsum khaws cia ntawm 4 ℃, tsis txhob khov ntawm -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR yuav tsum khaws cia ntawm -20 ℃ hauv qhov tsaus ntuj;Yog tias siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm luv (siv li ntawm 10 hnub).Qhov ntawd muaj tus cwj pwm zoo thiab ua rau cov neeg siv khoom txaus siab, peb lub koom haum txhim kho peb cov khoom zoo kom txaus siab rau cov neeg yuav khoom thiab tsom mus rau kev nyab xeeb, kev ntseeg siab, ib puag ncig specifications, thiab kev tsim kho tshiab ntawm Factory Supply China Convenient and Quick Rt-Step4 kev ua hauj lwm yuav ua tau peb cov kev txawj ntse Rt-StepR. ua siab ntev rau koj txoj kev txhawb nqa.Peb zoo siab tos txais koj tuaj yeem nres los ntawm peb lub vev xaib thiab tuam txhab thiab xa koj cov lus nug rau peb.
Factory SupplyTuam Tshoj TAQ DNA Polymerase, Qpcr, Peb tam sim no muaj cov kev daws teeb meem zoo tshaj plaws thiab cov kws tshaj lij kev muag khoom thiab kev pab cuam. Nrog rau kev txhim kho peb lub tuam txhab, peb muaj peev xwm muab cov neeg siv khoom zoo tshaj plaws, kev txhawb nqa zoo, kev pabcuam zoo tom qab muag.

Real Time PCR primer tsim qauv

Forward Primer thiab Reverse Primer

Rau Real Time PCR, primer tsim yog ib qho tseem ceeb heev.Primers muaj feem xyuam rau qhov tshwj xeeb thiab kev ua haujlwm ntawm PCR amplification, thiab tuaj yeem tsim nrog siv rau cov hauv qab no:

Primer ntev: 18-30bp.

GC cov ntsiab lus: 40-60%.

Tus nqi Tm: Primer tsim software, xws li Primer 5, tuaj yeem muab tus nqi Tm ntawm primer.Tm qhov tseem ceeb ntawm cov dej ntws thiab cov dej hauv qab yuav tsum nyob ze li qhov ua tau.Cov mis laij lej Tm kuj tseem siv tau: Tm = 4 °C (G + C) + 2 ° C (A + T).Thaum ua PCR, qhov kub thiab txias hauv qab tus nqi primer Tm ntawm 5 ° C feem ntau yog xaiv raws li qhov kub thiab txias (qhov sib piv ntawm qhov kub ntawm qhov annealing tuaj yeem nce qhov tshwj xeeb ntawm cov tshuaj tiv thaiv PCR).

Primers thiab PCR khoom:

Tsim primer PCR amplification khoom ntev yog nyiam dua 100-150bp.

Tsim primers nyob rau hauv lub thib ob structural cheeb tsam ntawm lub template yuav tsum tau zam kom ntau li ntau tau.

Zam kev tsim ntawm 2 lossis ntau qhov sib ntxiv ntawm lub hauv paus ntawm 3' kawg ntawm cov dej ntws thiab cov dej hauv qab.

Primer 3′ davhlau ya nyob twg puag tsis tuaj yeem nrog 3 sib law liag G lossis C.

Cov primers lawv tus kheej tsis tuaj yeem muaj cov qauv sib xyaw ua ke, txwv tsis pub tus qauv hairpin yuav raug tsim, cuam tshuam rau PCR amplification.

ATCG yuav tsum tau muab faib raws li qhov sib npaug li qhov ua tau hauv cov txheej txheem primer, thiab 3 'terminal puag yuav tsum raug zam raws li T.

Daim Ntawv Ntxiv 1: Cell Direct RT-qPCR Cov Khoom Siv Khoom Siv Ntxiv Pob

1.Cell Lysis Solution