Tuam Tshoj direct PCR khoom Chaw tsim tshuaj paus thiab tsum |Foregene

direct PCR cov khoom siv

Kit Description:

Cov khoom siv no siv cov txheej txheem lysis tsis zoo kom tso tawm genomic DNA los ntawm zebrafish thiab lwm cov ntaub so ntswg ntawm cov ntses dej qab zib, tail fins lossis cov qe ntses kuaj rau PCR cov tshuaj tiv thaiv, yog li nws yog qhov tshwj xeeb tshaj yog rau kev kuaj kab mob loj.Cov txheej txheem ntawm kev tso genomic DNA los ntawm lysis tsis yog ua tiav hauv 10-30 feeb ntawm 65 ° C.Tsis muaj lwm yam txheej txheem xws li protein thiab RNA tshem tawm, thiab cov kab DNA tso tawm tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.

2 × PCR EasyTM Mix muaj kev ua siab ntev rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv, thiab tuaj yeem siv cov lysate ntawm cov qauv los kuaj ua tus qauv rau kev ua kom tau zoo thiab tshwj xeeb.Cov reagent no muaj ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.Siv ua ke nrog cov lysis tsis tuaj yeem tshawb xyuas cov qauv sai thiab yooj yim, thiab muaj cov yam ntxwv ntawm kev kub siab, muaj zog tshwj xeeb, thiab kev ruaj ntseg zoo.

D-Taq DNA polymerase yog DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau cov tshuaj PCR ncaj qha.D-Taq DNA polymerase muaj kev ua siab ntev rau ntau yam PCR cov tshuaj tiv thaiv, thiab tuaj yeem ua kom muaj txiaj ntsig zoo ntawm DNA hauv ntau cov tshuaj tiv thaiv nyuaj, thiab qhov kev nthuav dav tuaj yeem ncav cuag 2Kb / min, uas yog tshwj xeeb tshaj yog haum rau Direct PCR cov tshuaj tiv thaiv.

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Product Detail

Khoom cim npe

Peb niaj hnub ua peb tus ntsuj plig ntawm "Innovation coj txoj kev loj hlob, Kev ua tau zoo tshaj plaws hauv kev noj qab haus huv, Kev tswj hwm kev tshaj tawm thiab kev lag luam tau txiaj ntsig, Cov qhab nia nyiam cov neeg yuav khoom rau cov khoom siv PCR ncaj qha, Peb tau nyob rau hauv kev saib ua ntej los koom tes nrog txhua qhov kev cia siab los ntawm hauv tsev thiab txawv teb chaws.Ntxiv mus, kev ua tiav cov neeg siv khoom yog peb txoj kev nrhiav mus ib txhis.
Peb niaj hnub ua peb tus ntsuj plig ntawm "Innovation coj txoj kev loj hlob, Ua tau zoo ua qee yam kev noj qab haus huv, Kev tswj hwm kev tshaj tawm thiab cov txiaj ntsig kev lag luam, Cov qhab nia zoo nyiam cov neeg yuav khoom rauDirect Pcr khoom siv, Peb tau tos ntsoov rau kev koom tes nrog koj rau peb cov kev sib nrig sib pab thiab kev loj hlob sab saum toj.Peb lav zoo, yog tias cov neeg siv khoom tsis txaus siab rau cov khoom zoo, koj tuaj yeem rov qab tsis pub dhau 7 hnub nrog lawv cov thawj lub xeev.

Specifications

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv

Ntu I	Tsis pub FP
	Foregene Protease
	6 × DNA Loading Buffer
Ntu II	2 × PCR Ib qho yooj yim^TMSib tov
Cov lus qhia

Nta & qhov zoo

■ Tsis tas yuav siv sijhawm ntev thiab kim purification DNA.

■ Cov qauv xav tau me me, tsawg li 1mg ntses caudal fin lossis 10 qe ntses tuaj yeem kuaj tau.

■ Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab kev sib tsoo, thiab kev ua haujlwm yooj yim.

■ Optimized PCR system ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv.

Cov khoom siv parameter

Daim ntawv thov: Zebra ntses thiab lwm yam ntses dej tshiab.

DNA tso tawm los ntawm cov qauv lysis: tsuas yog siv los ua PCR template.

Cov khoom siv tuaj yeem siv rau lub hom phiaj hauv qab no: kev txheeb xyuas ntawm transgenosis, tsiaj genotyping, thiab lwm yam.

Kev khiav haujlwm

wk-flw-zebra-fish-direct-PCR

Cia thiab Txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum khaws cia ntawm 2-8 ℃.

Lub reagent Buffer FP tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav;Nws tuaj yeem khaws cia ntawm 2-8 ℃ rau kev cia ntev dua.

v Reagent Foregene Protease muaj cov mis tshwj xeeb.Txhawm rau ua kom nws cov haujlwm thiab ruaj khov, thov khaws cia ntawm 4 ℃ rau 12 lub hlis.

vReagent 6 × DNA Loading Buffer tuaj yeem khaws cia ntawm 4 ℃ lossis -20 ℃ rau lub sijhawm ntev.

Ntu II ntawm cov khoom siv no yuav tsum khaws cia ntawm -20 ℃.

vReagent 2 × PCR EasyTM Mix, yog siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm luv luv (siv li ntawm 10 hnub).

Peb niaj hnub ua peb tus ntsuj plig ntawm "Innovation coj txoj kev loj hlob, zoo siab ua qee yam kev noj qab haus huv, Kev tswj hwm kev tshaj tawm thiab cov txiaj ntsig kev lag luam, Cov qhab nia nyiam cov neeg yuav khoom rau luv nqi Tuam Tshoj Cdv AG Lub Canine Distemper Virus Antigen Rapid Test Kit, Peb tau nyob ntawm qhov saib ua ntej rau kev koom tes nrog txhua qhov kev cia siab los ntawm hauv tsev thiab txawv teb chaws.Ntxiv mus, kev ua tiav cov neeg siv khoom yog peb txoj kev nrhiav mus ib txhis.
Loj Loj Tuam Tshoj Test Card, Test Strips, Peb tau tos ntsoov rau kev koom tes nrog koj mus rau peb cov kev sib nrig sib pab thiab kev loj hlob sab saum toj.Peb lav zoo, yog tias cov neeg siv khoom tsis txaus siab rau cov khoom zoo, koj tuaj yeem rov qab tsis pub dhau 7 hnub nrog lawv cov thawj lub xeev.

Yav dhau los: High quality / kev ncaj ncees tag nrho RNA cais cov khoom siv

Tom ntej: Factory ncaj qha khoom Tuam Tshoj Sab saum toj Hom Neeg Caij Tsheb Log Tsheb PCR Trailer Tire 155r12 185r14c 195r14c 205/55r16 215/45r17

Sau koj cov lus ntawm no thiab xa tuaj rau peb