Peb tau dhau los ua cov chaw tsim khoom.Yeej feem ntau ntawm cov ntawv pov thawj tseem ceeb ntawm nws txoj kev ua lag luam rau Kev txo nqi loj Tuam Tshoj High Sensitivity One-Step Probe Rt-QpcrCov Khoom Siv V2, Zoo yog lub Hoobkas 'lub neej, tsom rau cov neeg siv khoom' thov yog lub hauv paus ntawm lub tuam txhab kev muaj sia nyob thiab kev loj hlob, Peb ua raws li kev ncaj ncees thiab kev ntseeg siab ua haujlwm tus cwj pwm, tos ntsoov rau koj tuaj!
Peb tau dhau los ua cov chaw tsim khoom.Wining feem ntau hauv cov ntawv pov thawj tseem ceeb ntawm nws txoj kev ua lag luam rauTuam Tshoj TAQ DNA Polymerase, Qpcr, Peb lub tuam txhab cog lus: tus nqi tsim nyog, lub sijhawm luv luv thiab kev pabcuam tom qab muag zoo, peb kuj zoo siab tos txais koj tuaj xyuas peb lub Hoobkas txhua lub sijhawm koj xav tau.Xav tam sim no peb muaj kev lom zem thiab ntev ua lag luam ua ke !!!

Cov lus piav qhia

Cov khoom siv no siv cov txheej txheem lysis tsis zoo uas tuaj yeem tso tawm RNA sai sai los ntawm cov qauv ntawm tes rau RT-qPCR cov tshuaj tiv thaiv, yog li tshem tawm cov sijhawm siv sijhawm thiab siv zog RNA purification txheej txheem.RNA template tuaj yeem tau txais hauv 7 feeb xwb.5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR reagents muab los ntawm cov khoom siv tuaj yeem tau txais cov txiaj ntsig PCR sai thiab muaj txiaj ntsig zoo.

5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR muaj zog inhibitor kam rau ua, thiab lysate ntawm cov qauv siv tau los ua tus qauv rau RT-qPCR ncaj qha.Cov khoom siv no muaj qhov tshwj xeeb RNA high-affinity Foregene reverse transcriptase, thiab Kub D-Taq DNA polymerase, dNTPs, MgCl₂, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.

Specifications

200 × 20μl Rxns, 1000 × 20μl Rxns

Cov khoom siv

Nta & qhov zoo

■ Yooj yim thiab siv tau: nrog Cell Direct RT thev naus laus zis, cov qauv RNA tuaj yeem tau txais hauv 7 feeb xwb.

■ Cov qauv xav tau me me, tsawg li 10 lub hlwb tuaj yeem kuaj tau.

■ Kev xa tawm siab: nws tuaj yeem ntes RNA sai sai hauv cov kab mob hauv 384, 96, 24, 12, 6-zoo daim hlau.

■ DNA Eraser tuaj yeem tshem tawm cov genomes sai sai, txo qhov cuam tshuam rau cov txiaj ntsig kev sim tom ntej.

■ Optimized RT thiab qPCR system ua rau ob-kauj ruam RT-PCR thim rov qab tau zoo dua thiab PCR ntau dua, thiab tiv taus ntau dua rau RT-qPCR cov tshuaj tiv thaiv.

Daim ntawv thov Kit

Scope ntawm daim ntawv thov: kab lis kev cai.

- RNA tso tawm los ntawm cov qauv lysis: tsuas yog siv tau rau RT-qPCR qauv ntawm cov khoom siv no.

- Cov khoom siv tuaj yeem siv rau cov hom phiaj hauv qab no: kev tshuaj ntsuam gene qhia, kev txheeb xyuas ntawm siRNA-mediated gene silencing effect, tshuaj ntsuam xyuas tshuaj, thiab lwm yam.

Daim duab

Cia thiab Txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum khaws cia ntawm 4 ℃;Ntu II yuav tsum khaws cia ntawm -20 ℃.

Foregene Protease Plus II yuav tsum khaws cia ntawm 4 ℃, tsis txhob khov ntawm -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR yuav tsum khaws cia ntawm -20 ℃ hauv qhov tsaus ntuj;yog siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm luv luv (siv tsis pub dhau 10 hnub).Wining the majority in the crucial certifications of its market for Big discounting China High Sensitivity One-Step Probe Rt-Qpcr Kit V2, Zoo yog Hoobkas 'lub neej , Tsom ntsoov rau cov neeg siv khoom 'xav tau yog lub hauv paus ntawm tuam txhab ciaj sia thiab kev loj hlob, Peb ua raws li kev ncaj ncees thiab kev ncaj ncees ua hauj lwm tus cwj pwm, tos ntsoov rau koj los !
Kev txo nqi lojTuam Tshoj TAQ DNA Polymerase, Qpcr, Peb lub tuam txhab cog lus: tus nqi tsim nyog, lub sijhawm luv luv thiab kev pabcuam tom qab muag zoo, peb kuj zoo siab tos txais koj tuaj xyuas peb lub Hoobkas txhua lub sijhawm koj xav tau.Xav tam sim no peb muaj kev lom zem thiab ntev ua lag luam ua ke !!!

QuickEasyTM Cell Direct RT-qPCR Kit -Tauman

No.DRT-01021/01022

Rau cell ncaj qha RT-qPCR siv ≤ 1000,000 hlwb

Khoom taw qhia

Cov khoom no siv cov txheej txheem lysis tsis zoo kom tso tawm RNA sai sai los ntawm cov qauv ntawm tes rau RT-qPCR cov tshuaj tiv thaiv, tshem tawm cov sijhawm siv thiab ua haujlwm RNA purification txheej txheem, thiab tsuas yog 7 feeb kom tau txais cov qauv RNA uas yuav tsum tau, nrog 5 × Direct RT Mix, 2 × Direct qPCR Mix-Taqman tau sai sai thiab ua tau zoo los ntawm lub sijhawm tiag tiag.

5 × Direct RT Mix thiab 2 × Direct qPCR Mix-Taqman muaj zog inhibitor kam rau ua thiab tuaj yeem ua tau zoo thim rov qab thiab tshwj xeeb amplification siv cov lysate ntawm cov qauv los ntsuas raws li tus qauv.Cov reagent muaj Foregene Reverse Transcriptase, Kub D-Taq DNA Polymerase, dNTPs, MgCl₂, Reaction Buffer, PCR Optimizer thiab Stabilizer, uas tuaj yeem siv nrog lysis buffer kom sai thiab yooj yim kuaj cov qauv, thiab muaj cov yam ntxwv ntawm siab rhiab heev, tshwj xeeb thiab ruaj khov.

Khoom nta

Yooj yim, siv tau Cell Direct RT thev naus laus zis uas siv sijhawm li 7 feeb kom tau txais cov qauv RNA.

Cov qauv xav tau me me, thiab tsawg kawg ntawm 10 cov kab lis kev cai tuaj yeem siv rau kev sim.

High throughput rau ceev RNA tau ntawm cov kab mob hlwb xws li 384, 96, 24, 12, thiab 6-zoo daim hlau.

DNA Eraser tuaj yeem tshem tawm cov genomes sai sai, txo qhov cuam tshuam rau cov txiaj ntsig kev sim tom ntej.

Optimized RT thiab qPCR systems ua rau ob-kauj ruam RT-PCR nrog ntau qhov rov qab hloov dua siab tshiab, tshwj xeeb, thiab muaj zog RT-qPCR cov tshuaj tiv thaiv inhibitor kam rau ua.

Daim ntawv thov Kit

Scope ntawm daim ntawv thov: Cultured cells.

Sample lysis txhais RNA: tsuas yog siv ob-kauj ruam RT-qPCR template.

Cov khoom siv tuaj yeem siv rau lub hom phiaj hauv qab no: kev tshuaj ntsuam xyuas cov noob caj noob ces, kuaj allele, tshuaj xyuas tshuaj, thiab lwm yam.

Cov khoom txwv

Amplified fragments ≤ 300 bp.

Cov khoom siv yog siv rau cov kab mob tshiab tshiab.

Khoom zoo tswj

Raws li FOREGENE's Total Quality Management System, txhua pawg ntawm Cell Direct RT-qPCR series cov khoom siv tau nruj me ntsis sim ntau zaus kom ntseeg tau tias muaj kev ntseeg siab thiab ruaj khov ntawm qhov zoo ntawm txhua pawg ntawm cov khoom siv.

Cov khoom siv

*:Cell Lysis, 5 × Direct RT Mix, 2 × Direct qPCR Mix-Taqman tuaj yeem yuav nyias, cov ntsiab lus muaj nyob rau hauv Daim Ntawv Ntxiv 1 (PAGE 13).

Cia tej yam kev mob

1. Cov xwm txheej shipping

Tag nrho cov txheej txheem ntawm kev thauj mus los ntawm lub thawv ntim dej khov, kom ntseeg tau tias cov khoom siv nyob rau hauv lub xeev <4 ° C.

2. Cia tej yam kev mob

Khaws qhov chaw I ntawm 4 ° C thiab Ntu II ntawm -20 ° C.

Foregene Protease Plus II yuav tsum khaws cia ntawm 4 ° C, tsis khov ntawm -20 ° C.

Reagent 2 × Direct qPCR Mix-Taqman khaws cia ntawm -20 ° C, lossis ntawm 4 ° C rau kev siv sijhawm luv yog siv ntau zaus (hauv 10 hnub).

Cov ntaub ntawv ntawm cov khoom siv

Buffer CL: Muab ib puag ncig xav tau rau cov tshuaj tiv thaiv cell lysis.

Buffer ST: Tshem tawm cov tshuaj nquag hauv lysate kom tsis txhob cuam tshuam rau RT tom ntej.

DNA Eraser: DNA tshem tawm, cov nyhuv ntawm tshem tawm cov genome ntawm kev sim tom ntej.

5 × Direct RT Mix: Muaj RNA affinity siab Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, stabilizers, enhancers, optimizers, thiab rov qab transcription primers rau kev pom zoo (Random Primer, Oligo(dT)₁₈Primer).

Foregene Protease Plus II: Nyob rau hauv cov ntsiab lus ntawm lysis buffer, hlwb yog lysed tso tawm nucleic acids.

2 × Direct qPCR Mix-Taqman: Cov reagent no muaj Kub D-Taq DNA Polymerase, dNTPs, MgCl₂, cov tshuaj tiv thaiv tsis zoo, PCR optimizer, thiab stabilizer.

20 × ROX Reference Dye: Feem ntau siv los ntawm Real Time PCR amplification instruments ntawm ABI, Stratagene thiab lwm lub tuam txhab, nws yog siv los kho qhov sib txawv ntawm PCR hlab thiab cov hlab los ntawm PCR dosing yuam kev.Lub 20 × ROX Reference Dye concentration xav tau rau cov cuab yeej sib txawv yog qhov sib txawv, thiab tus neeg siv tuaj yeem ntxiv nws raws li qhov pom zoo ntawm qhov ntsuas.

RNase-Dawb ddH₂O: RNase-free sterilized ultra ntshiab dej rau ob-kauj ruam RT-qPCR cov tshuaj tiv thaiv.

Cov kev ceev faj:(Nco ntsoov nyeem cov lus ceeb toom ua tib zoo ua ntej siv cov khoom siv)

Ua tib zoo saib xyuas txoj kev ua haujlwm ntawm qhov kev sim kom tsis txhob muaj kev sib kis ntawm cov qauv.

Ua tib zoo saib xyuas qhov huv ntawm qhov chaw sim thiab cov khoom siv kom tsis txhob muaj RNase paug thiab RNA degradation.

Nqa cov qauv tshiab los yog khaws cia zoo ntawm tes thiab tsis txhob siv cov qauv cell rov qab khov-thawed.

5 × Direct qPCR Mix, 2 × Direct qPCR Mix-Taqman yuav tsum tsis txhob rov qab khov-thaw, txwv tsis pub nws yuav cuam tshuam rov qab cov ntaub ntawv thiab PCR efficiency.

Npajrationsua ntejkev ua haujlwm

Nco ntsoov nyeem cov lus qhia ua tib zoo ua ntej siv cov khoom siv no.Cov Khoom Siv Cell Direct RT-qPCR yog qhov yooj yim, yooj yim, thiab nrawm rau kev khiav lag luam, thiab cov lus qhia muab cov ntaub ntawv tiav txog tag nrho cov khoom siv thiab yuav siv li cas kom raug.Thov npaj cov khoom siv thiab cov khoom siv sim ua ntej siv.

Cov khoom siv thiab cov khoom siv sim

◆ Kab lis kev cai.

◆ 1.5 ml lossis 2 ml, RNase-/DNase-Free centrifuge tube, RNase-/DNase-Free tip, 0.2 ml sterile qPCR tube.

◆ qPCR tshuab, pipette, tabletop centrifuge (≥13.400 Nws×g) (nyob ntawm kev xav tau kev sim), thiab lwm yam.

Kev nyab xeeb

◆ Cov khoom no yog siv rau kev tshawb fawb txog kev tshawb fawb nkaus xwb, thov tsis txhob siv rau kev siv tshuaj, chaw kho mob, zaub mov thiab tshuaj pleev ib ce.

◆ Thaum siv tshuaj, hnav khaub ncaws hauv chaw kuaj mob, hnab looj tes, tsom iav, thiab lwm yam.

Kev ua haujlwmcov lus qhia

Cell Lysis systems, RT systems, thiab qPCR cov tshuaj tiv thaiv cov tshuaj ntxiv pob khoom tuaj yeem yuav tau nyias, kom paub meej hauv Daim Ntawv Ntxiv 1 (PAGE 13).

Kev qhia ua haujlwm

A: Qauv RNA tso tawm

1.Cells raug pretreated: Ntxuav lub cell kab lis kev cai phaj nrog txias PBS, ces lyse lub hlwb (10-10⁶), 10⁶ tshaj li tus nqi ntawm cov hlwb, pom zoo Foregene lub Cell ib RNA Isolation Kit the Total (DE-03111) lossis Tsiaj Tag Nrho RNA Isolation Kit (DE-03011) rau RNA rho tawm thiab purification.

1.1.Adherent cells (24-zoo phaj ua piv txwv)

1.1.1.Txiav txim seb tus naj npawb ntawm cov cell hauv txhua qhov dej, txiav txim siab tias cov cell yog 1 × 10⁵, thiab siv lub pipette tshem tawm kab lis kev cai nruab nrab ntawm kab lis kev cai tais.

1.1.2.Ntxiv 200 μl ntawm pre-chilled 1 × PBS rau txhua qhov dej.Tsis txhob tso dej ntau zaus thiab tshem PBS tawm ntawm qhov dej.Tilt lub phaj thiab tshem tawm PBS ntau npaum li sai tau.Nkag mus rau kauj ruam 2.

1.1.3.Cov kab lis kev cai ntawm tes sib txawv lossis tus lej siv lub rooj 1-1 hauv lub cell kab lis kev cai tais tau ntxiv precooled 1 × PBS rau kev ntxuav ntawm tes.

Table1-1: PBS ntau npaum li cas rau cov xov tooj sib txawv

Nco tseg:Txhawm rau kom muaj cov kab mob ruaj khov,coob tus ntawm tes tsis zam thaum ntxuav.

1.2.Cov hlwb raug tshem tawm lossis cov kab mob sib txuas ua ke nyob rau hauv daim hlau uas tsis yog ntxeem tau

1.2.1.Adherent cells cultured in non-multi well plates (suspension cells pib los ntawm cov kauj ruam tom ntej 1.2.2), sau thiab cais cov hlwb raws li txoj kev sau ntawm tes, thiab muab tso rau hauv kab lis kev cai phaj lossis centrifuge raj;yog siv trypsinization, yuav tsum tau centrifugation los sau cov hlwb thiab tshem tawm cov seem seem ntawm trypsin, ntxiv PBS resuspended hlwb rau hauv ib lub hlwb kom disperse lub hlwb.

1.2.2.Tom qab cov xov tooj suav, aliquoted hlwb 1 × 10⁵ ib mus rau centrifuge raj, sau cov hlwb los ntawm centrifugation ntawm 1000 × g rau 10 min.

1.2.3.Ntxiv 200 μl PBS rau lub centrifuge raj, tsis txhob pipet dua, thiab ncaj qha aspirate lub PBS.mus rau kauj ruam 2. (Yog tias nyuaj rau nag lossis daus thiab cov hlwb tau rov ua dua, tej zaum yuav tau ua 1000 × g centrifuged 10 min tom qab muab cov supernatant pov tseg, lub xov tooj ntawm tes mus rau kauj ruam 2)

2.Cell lysis: Tshem tawm Buffer CL, nws qhov kub thiab txias sib npaug rau chav sov, DNA Eraser thiab Foregene Protease Plus II, raws li cov lus hauv qab no 1-2 npaj lysis system: (Lysis tov yog npaj siv).

Table 1-2: cleavage system npaj (Ceeb toom: hauv kev npaj rau dej khov)

3.( 24 -zoo phaj raws li ib qho piv txwv ) Pipette 200 μl ntawm cell lysis master mix rau hauv txhua lub qhov dej, Rov qab tshuab 5-10 zaug, Incubate ntawm chav tsev kub (20-25 ℃) rau 5 min.

Nco tseg:Txhawm rau kom tsis txhob tsim cov npuas, thov thaum pipette scale tau hloov kho rau 200μl lossis tsawg dua.Cov hlwb yuav tshwm sim huab tom qab lysis, uas yog qhov qub.

4.(24 -zoo phaj raws li ib qho piv txwv) yog ntxiv rau hauv cov kua 20 μl Buffer ST ( txawv lysis systems Buffer ST ntxiv nyob rau hauv ib tug npaum li cas qhia nyob rau hauv Table 1-3), rov pipetting 5-10 zaug, nyob rau hauv chav tsev kub (20-25 ℃) tau incubated rau 2 min.

Nco tseg:Lub pipette taub pov tseg hauv qab ntawm qhov chaw, xyuas kom meej tias cov lysate tau ntxiv,kom tsis txhob tsim cov npuas, thov thaum pipetting pipette nplai tau hloov kho rau 200μl lossis tsawg dua.

Table 1-3:Ntxiv Buffer ST

5.Cov lysate yog siv rau kev sim RT-qPCR tom ntej.Yog tias qhov kev sim tom ntej tsis tuaj yeem ua tiav raws sijhawm, thov khaws cia rau hauv dej khov tsis pub dhau 2 teev, thiab khaws cia ntawm -20 ℃ lossis -80 ℃ (tsis pub ntau tshaj peb lub hlis).

B: RT system npaj

1. Tshem tawm 5 × Direct RT Mix thiab muab tso rau hauv ib da dej dej khov, cia nws yaj lawm, thiab maj mam sib tov rau tom qab siv;tshem tawm RNase-Free ddH2O thiab yaj nws thiab muab tso rau hauv ib da dej dej khov rau tom qab siv.Npaj cov tshuaj tiv thaiv ntawm cov dej khov raws li Table 2-1 hauv qab no.

Table 2-1: Kev npaj ntawm RT cov tshuaj tiv thaiv kab mob

2.Tom qab ua tiav cov txheej txheem tsim, maj mam sib xyaw thiab centrifuged luv luv hauv cov lus hauv qab no 2 -2 cov tshuaj tiv thaiv RT cov tshuaj tiv thaiv.

Table 2-2: RT Reaction condition setting

3.Tom qab ua tiav cov tshuaj tiv thaiv, cov khoom siv tshuaj tiv thaiv tau muab tso ncaj qha rau ntawm cov dej khov rau qPCR, thov muab tso rau lub sijhawm ntev -20 ℃ lossis -80 ℃ .

Nco tseg: Vim yog siv cov qauv tsis huv, cov hmoov av dawb tuaj yeem tshwm sim hauv cov khoom siv rov qab.Qhov no yog ib qho tshwm sim.Centrifuge tus supernatant tam sim ntawd rau kev sim tom ntej.

Qhov tshwm sim RT cov tshuaj tiv thaiv yog ntxiv rau cov kauj ruam tom ntej Real Time PCR cov tshuaj tiv thaiv, nws raug nquahu kom ntxiv cov nyiaj li ntawm 10-30% ntawm cov tshuaj tiv thaiv.

C: qPCR tshuaj tiv thaiv kev npaj

1. Tsim nyog tus nqi ntawm B npaj nyob rau hauv kauj ruam cDNA template raws li cov lus hauv qab no 3-1 los npaj cov tshuaj tiv thaiv.

Nco tseg: Tus nqi ntawm cDNA tus qauv tso nyiaj rau 10-30% ntawm qPCR system.Piv txwv li, nyob rau hauv 20μl qPCR system, ntxiv 2-6 μl ntawm lysis buffer, tab sis tsis ntau tshaj 6 μl.

2.Qhov optimization zoo qPCR tej yam kev mob (Annealing kub, thiab lwm yam.) rau qPCR cov tshuaj tiv thaiv (Cov tshuaj tiv thaiv tej yam kev mob muab rau ntawm Table 3-2).

Nco tseg: Sim siv cov xwm txheej zoo rau qPCR cov tshuaj tiv thaiv kom tau txais txiaj ntsig zoo dua.

Table 3-1: Kev npaj ntawm PCR cov tshuaj tiv thaiv kab mob

1 *: Primer concentration tuaj yeem hloov kho hauv thaj tsam ntawm 50-900 nM thaum primer cov tshuaj tiv thaiv kev ua haujlwm tsis zoo.

Lus Cim: QPCR system tuaj yeem hloov kho raws li kev xav tau ntawm kev sim thiab fluorescence cycler qauv.Rau qPCR hauv 50μl system, kho cov tshuaj reagent proportionally raws li 20μl system.

2 *: Xaiv qhov tsim nyog kawg concentration ntawm ROX Reference Dye raws li fluorescence quantitative thermal cycler.Qhov tsim nyog tshaj ROX Reference Dye concentrations rau fluorescence quantitative cyclers muaj nyob rau hauv cov lus hauv qab no:

Table 3-2: qPCR cov kev mob tshwm sim tau muab

Nco tseg: Txhawm rau kom tau txais cov txiaj ntsig zoo tshaj plaws qPCR, gradient PCR tuaj yeem siv los ua kom zoo dua cov tshuaj tiv thaiv rau cov qauv sib txawv thiab cov primers sib txawv.PCR cov kev mob tshwm sim txawv nyob ntawm qhov ntsuas fluorescence analyzer, template, primer, thiab lwm yam. Hauv kev ua haujlwm tshwj xeeb, qhov kev pom zoo ntawm cov tshuaj tiv thaiv yuav tsum tau tsim raws li cov xwm txheej tshwj xeeb ntawm fluorescence quantitative thermal cycler, hom qauv, qhov loj ntawm qhov seem ntawm qhov txaus siab, lub hauv paus sib lawv liag ntawm cov khoom tawg thiab GC cov ntsiab lus thiab qhov ntev ntawm primers, nrog rau lub sij hawm ntawm qhov kub thiab txias.

Real Time PCR primer tsim qauv

Forward Primer thiab Reverse Primer

Rau Real Time PCR, primer tsim yog ib qho tseem ceeb heev.Primers muaj feem xyuam rau qhov tshwj xeeb thiab kev ua haujlwm ntawm PCR amplification, thiab tuaj yeem tsim nrog siv rau cov hauv qab no:

◆ Primer ntev: 18-30bp.

◆ GC cov ntsiab lus: 40-60%.

◆ Tm tus nqi: Primer tsim software, xws li Primer 5, tuaj yeem muab tus nqi Tm ntawm primer.Tm qhov tseem ceeb ntawm cov dej ntws thiab cov dej hauv qab yuav tsum nyob ze li qhov ua tau.Cov mis laij lej Tm kuj tseem siv tau: Tm = 4 °C (G + C) + 2 ° C (A + T).Thaum ua PCR, qhov kub thiab txias hauv qab tus nqi primer Tm ntawm 5 ° C feem ntau yog xaiv raws li qhov kub thiab txias (qhov sib piv ntawm qhov kub ntawm qhov annealing tuaj yeem nce qhov tshwj xeeb ntawm cov tshuaj tiv thaiv PCR).

◆ Primers thiab PCR khoom:

◆ Tsim primer PCR amplification khoom ntev yog qhov zoo dua 100-150bp.

◆ Tsim cov primers nyob rau hauv lub thib ob structural cheeb tsam ntawm lub template yuav tsum tau zam kom ntau li ntau tau.

◆ Zam kev tsim ntawm 2 lossis ntau dua cov hauv paus ntxiv ntawm 3′ qhov kawg ntawm cov dej ntws thiab cov dej hauv qab.

◆ Primer 3′ terminal base tsis tuaj yeem nyob nrog 3 txuas ntxiv G lossis C.

◆ Cov primers lawv tus kheej tsis tuaj yeem muaj cov qauv sib txuas ntxiv, txwv tsis pub yuav muaj cov qauv hairpin, cuam tshuam rau PCR amplification.

◆ ATCG yuav tsum tau muab faib kom sib npaug li qhov ua tau hauv cov txheej txheem primer, thiab 3′ lub davhlau ya nyob twg yuav tsum raug zam raws li T.

Cov ntawv ntxiv1:Cnws DirectRT-qPCR Kit component ntxiv pob

1.Cell Lysis Solution