Nyob zoo, phooj ywg!
Tom qab ua nucleic acid extraction tau ntau xyoo, txhua tus paub zoo txog qhov tseeb tias qhov zoo ntawm nucleic acid extraction yog tus yuam sij rau kev ua tiav thiab raug ntawm cov kev sim tom ntej.
Ntau zaus, peb tsuas yog saib xyuas seb qhov piv ntawm OD260 / OD280 yog nyob rau hauv qhov kev pom zoo.Ib yam li no…
Raws li cov lus hais, koj tsis tuaj yeem hais lus ntau dhau, thiab koj tsis tuaj yeem zoo siab thaum ntxov.
Ua ntej mloog Editor qhia koj ib zaj dab neeg me me!
Xws li cov dab neeg zoo nkauj feem ntau tshwm sim hauv cov chaw kuaj mob uas ua cov nucleic acid extraction.
Koj xav tias koj tsuas yog coj RNA xwb, Thov txim!
Qhov tseeb, nws tsuas yog tias koj tsis paub tias Mr Protein zais zais zais.
Koj puas xav tias koj xav ua kom Mr Protein tshwm sim nrog tsuas yog ib qho OD260/OD280?
hluas dhau lawm!Yooj yim dhau!
Cia peb siv txoj kev staining nyiaj rhiab tshaj plaws los pab koj pom cov proteins zais hauv RNA!
| Khoom siv | OB 260/280 | Myooj yim ntsuas |
| Foregene | 2.09 Nws | Thermo ScientificTMNanoDropTMIb Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 ib |
Ntev, ntev dhau los, protein thiab RNA yog cov phooj ywg zoo.
Yawg Protein txaj muag dua.
Yog li nws yeej ib txwm ua haujlwm thiab zoo siab RNA ua si nrog nws.
Ib hnub.
RNA hais rau Mr Protein tias nws yuav raug coj los ntawm lwm tus, thiab Mr Protein tau tu siab heev.
RNA kuj tsis txaus siab rau Mr protein, thiab hais tias: "Yuav ua li cas kuv nyiag koj mus, koj nkaum hauv kuv khaub ncaws, sim tsis txhob pom los ntawm lwm tus, tsis ua li cas?"
Mr. Protein tsoo rau hauv kev luag nyav thiab zoo siab pom zoo.
Hnub yug, Mr Protein yeej nkaum hauv RNA cov khaub ncaws thiab tsis pom.Nws tau raws tus neeg saws ntawd rov qab los tsev thiab ua lub neej zoo siab txij thaum ntawd los.
Kev sib piv ntawm RNA extraction nrog Fcov oregeneCellTag nrhoRNA Extraction Kit
thiab T Company's Trizol Reagent
Peb tuaj yeem pom tseeb qhov kev sib tw ntawm txoj kab 4 (Trizol), thaum txoj kab 3 (Foregene) kuj huv si.Cov txiaj ntsig tau lees paub tias RNA protein impurities rho tawm los ntawm txoj kev Trizol tseem muaj ntau tee!Thiab txoj kab 3 (Foregene) yuav luag tsis muaj protein ntau impurities vim tus neeg sim siv cov khoom siv Foregene RNA rho tawm!(Qhov kev tshaj tawm tuaj tsis tau xav txog)
Xav paub ntxiv:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Post lub sij hawm: Feb-10-2022
