Keeb kwm
Nyob rau hauv xyoo tas los no, extracellular vesicles (EVs) tau nyiam tib neeg txoj kev xav raws li lub peev xwm kho mob;Txawm li cas los xij, qhov kev kho mob ntawm EVs ntawm endometriosis tsis tau tshaj tawm.Endometriosis yog ib hom kab mob gynecological uas tsis zoo uas cuam tshuam rau 10-15% ntawm cov poj niam ntawm cov menyuam muaj hnub nyoog, ua rau muaj ntau yam tsos mob, ua rau muaj kev poob qis hauv lub neej thiab lub nra hnyav.
Tshooj Lus Taw Qhia
Thaum Lub Xya Hli 20, 2021, pawg tshawb fawb ntawm Xib Fwb Wang Guoyun los ntawm Qilu Tsev Kho Mob ntawm Shandong University tau luam tawm cov ntaub ntawv tshawb fawb hu ua "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis" on Frontiers in immunology, uas tau tham txog qhov derivation ntawm M1.Qhov ua tau ntawm nanovesicles (NVs) hauv kev kho mob endometriosis.
Kab lus no siv cov txheej txheem txuas ntxiv txuas ntxiv los npaj M1NVs, thiab siv cov txheej txheem kev coj noj coj ua los kawm txog kev hloov pauv hauv angiogenesis, tsiv teb tsaws, ntxeem tau thiab lwm yam ntsuas ntawm eutopic endometrial stromal cells (EM-ESCs) los ntawm cov neeg mob endometriosis.Nyob rau tib lub sijhawm, tus qauv nas ntawm endometriosis tau tsim, thiab cov nas tau kho nrog PBS, MONVs lossis M1NVs, raws li, txhawm rau ntsuas qhov ua tau zoo thiab kev nyab xeeb ntawm M1NV hauv kev kho mob endometriosis.
Cov txiaj ntsig tau pom tias hauv vitro M1NVs tuaj yeem ncaj qha lossis tsis ncaj qha cuam tshuam kev tsiv teb tsaws thiab cuam tshuam ntawm EM-ESCs, thiab inhibit angiogenesis.Hauv cov qauv nas, M1NVs inhibit qhov tshwm sim ntawm endometriosis los ntawm M2 macrophage reprogramming yam tsis ua rau lub cev puas tsuaj.Nws qhia tau hais tias M1NVs tuaj yeem cuam tshuam ncaj qha rau qhov tshwm sim ntawm endometriosis, thiab tseem tuaj yeem cuam tshuam los ntawm repolarizing M2 hom macrophages rau M1 hom.Yog li, kev siv M1NVs yuav yog ib txoj hauv kev tshiab rau kev kho mob endometriosis.
Foregene pab
Nyob rau hauv txoj kev tshawb no, vim hais tias M1NV tau npaj los ntawm tsis tu ncua squeezing M1 macrophages, tsab xov xwm siv qRT-PCR los xyuas cov pro-inflammatory yam thiab M1 macrophage markers iNOS, TNF-a thiab IL-6 mRNA hauv M1NV thiab M1 macrophages.txheeb ze ntau qhov kev hloov.Cov txiaj ntsig tau pom tias M1NVs muaj ntau qhov cuam tshuam rau mRNA thiab M1 macrophage cov cim, qhia tias M1NVs tuaj yeem khaws cov yam ntxwv ua haujlwm ntawm M1 hlwb.Txoj kev tshawb fawb no siv QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I ntawm Foregene
Cell Direct RT-qPCR cov khoom sivpaub meej

Daim ntawv thov scenarios:
 
1. Kev tswj cov noob caj noob ces thiab kev tsom xam, kev txheeb xyuas cov noob ntau dhau los yog cuam tshuam cuam tshuam, kev tshuaj xyuas tshuaj, thiab lwm yam;
2. Cov noob caj noob ces nrhiav kom tau cov hlwb uas nyuaj-rau-cultivable xws li thawj cov hlwb, qia hlwb, thiab paj hlwb;
3. Kev kuaj xyuas mRNA hauv cov qauv xws li exosomes thiab nanovesicles.
Nta: