Lub invention ntawm lub tshuab nqus tsev vacuum coj cov kev lag luam kiv puag ncig, kev nce qib ntawm cov computers tsim lub ntiaj teb digital tam sim no, thiab kev hloov kho thev naus laus zis yuav muaj tseeb coj mus rau lub sijhawm!Kev yug ntawmCell Direct RT-qPCR cov khoom sivntawm Foregene tab tom yuav coj lub sijhawm tshiab ntawm kev tshawb pom molecular!

7min Tau RNA template

Yog lawm, koj hnov ​​nws yog, tsis txhob ua xyem xyav, los ntawm cell mus rau RT-qPCR, tam sim no tsuas yog ob kauj ruam

Nres Qhov Teeb Meem

Q:Puas yog RNA extraction txaus ntawm qhov ceev ceev?Puas yuav muaj DNA paug?

A: Tsis txhob txhawj, peb cov khoom tau dhau los suav tsis txheeb qhov kev sib piv thiab kev txheeb xyuas, thiab tsis muaj qhov sib txawv piv nrog cov txiaj ntsig ntawm kev sim RT-qPCR ib txwm muaj.Tsis tas li ntawd, cov qauv loj me me, thiab qhov kev sim tuaj yeem ua tiav nrog tsawg kawg ntawm 10 kab lis kev cai.Txawm hais tias nws yog 6, 12, 24 lossis 96-zoo phaj, cov hlwb tuaj yeem raug lysed ncaj qha rau hauv lub qhov dej ntawm lub phaj, uas yooj yim thiab ceev.Nrog rau 5 × Direct RT Mix thiab 2 × Direct qPCR Mix nyob rau hauv cov khoom, RNA template yuav siv tau rau high-efficiency reversal thiab tshwj xeeb amplification, nrog siab rhiab heev, muaj zog tshwj xeeb thiab zoo stability.The rov qab transcriptase qPCR cov khoom yog li yooj yim.

Cell Direct RT-qPCR cov khoom sivTsis tsuas yog muaj cov txheej txheem lysis zoo tshaj plaws, tab sis kuj muaj qhov muaj zog tiv thaiv RT-qPCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv, uas ua rau tag nrho cov khoom siv muaj qhov txwv tsis pub tshaj, thiab nws tseem tuaj yeem tau txais txiaj ntsig zoo ntawm 10 hlwb.Qhov no yog ib kauj ruam RT qPCR cov khoom siv yooj yim heev.

Tam sim no muaj SYBR ntsuab qPCR thiab Taqman qPCR txoj kev muaj.