Nyob rau theem pib ntawm qhov tshwm sim, vim muaj kev txhim kho sai, kev kuaj mob sai ntawm cov neeg mob xav tias yog tus yuam sij rau kev tiv thaiv COVID-19.Qee qhov pom zoo nucleic acid nrhiav kom tau reagents muaj lub sijhawm luv luv, thiab muaj teeb meem xws li kev pom zoo sai sai, tsis txaus reagent optimization, thiab qhov sib txawv loj ntawm cov khoom siv;Cov teeb meem ntawm ntau lub chaw kuaj mob hauv ntau yam ntawm cov txheej txheem tshawb nrhiav nucleic acid kuj tuaj yeem cuam tshuam qhov tseeb ntawm cov txiaj ntsig nucleic acid nrhiav pom.Tsab ntawv xov xwm no yuav tsom mus rau cov kev sib txuas tseem ceeb thiab cov ntsiab lus hauv kev tshawb nrhiav SARS-CoV-2 nucleic acid tam sim no, thiab txheeb xyuas cov teeb meem ntawm qhov tsis zoo thiab qhov zoo rov kuaj xyuas ntawm lub chaw kuaj nucleic acid nrhiav thiab kho qhov tsis sib xws.

Principles of SARS-CoV-2 nucleic acid detection

SARS-CoV-2 yog tus kab mob RNA uas muaj cov kab mob genome ntawm kwv yees li 29 kb, nrog 10 genes, uas tuaj yeem ua tau zoo 10 cov proteins.Cov kab mob no yog tsim los ntawm RNA thiab cov protein, thiab txheej txheej sab nraud yog txheej txheej txheej uas muaj lipids thiab glycoproteins.Sab hauv, cov protein capsid qhwv RNA hauv nws, yog li tiv thaiv RNA yooj yim degradable (P1).

P1 Structure of SARS-COV-2

Cov kab mob nkag mus rau cov hlwb los ntawm cov xov tooj ntawm tes tshwj xeeb los ua kom muaj kab mob, thiab siv cov cell ua kom rov ua dua.

Lub hauv paus ntsiab lus ntawm kev kuaj pom tus kab mob nucleic acid yog txhawm rau nthuav tawm tus kab mob RNA los ntawm lub xov tooj ntawm tes lysate, thiab tom qab ntawd siv lub sijhawm tiag tiag fluorescent thim rov qab-polymerase saw cov tshuaj tiv thaiv (RT-PCR) txhawm rau kuaj pom.

Lub hauv paus ntsiab lus ntawm kev tshawb nrhiav yog siv primers thiab sojntsuam kom ua tiav "kev sib tw" ntawm nucleic acid sequences, uas yog, nrhiav nucleic acid sequence ntawm SARS-CoV-2 uas txawv ntawm lwm cov kab mob hauv thaj tsam li 30,000 lub hauv paus (qhov zoo sib xws ntawm nucleic acid rau lwm cov kab mob) "Low" probes), tsim.

Cov primers thiab kev sojntsuam tau zoo sib xws nrog thaj tsam tshwj xeeb ntawm SARS-CoV-2 nucleic acid, uas yog, qhov tshwj xeeb muaj zog heev.Thaum lub sij hawm tiag tiag fluorescent RT-PCR amplification tshwm sim ntawm cov qauv mus kuaj yog qhov zoo, nws ua pov thawj tias SARS-CoV-2 muaj nyob rau hauv cov qauv.Saib P2.

P2 Steps of SARS-CoV-2 nucleic acid determination (real-time fluorescent RT-PCR)

Cov xwm txheej thiab cov tseev kom muaj ntawm chav kuaj rau SARS-CoV-2 nucleic acid detection

Nucleic acid kuaj cov chaw soj nstuam yog qhov zoo tshaj plaws rau qhov tsis zoo ntawm qhov chaw ib puag ncig, thiab lawv yuav tsum tau ua tib zoo saib xyuas lub siab, ua kom huab cua ntws, thiab tshem tawm cov aerosols.Nucleic acid kuaj cov neeg ua haujlwm yuav tsum muaj cov kev tsim nyog sib xws, tau txais cov kev cob qhia cuam tshuam polymerase chain reaction thiab dhau qhov kev ntsuam xyuas.Lub chaw soj nstuam yuav tsum tau tswj xyuas nruj me ntsis, zoned nyob rau hauv qhov chaw, thiab cov neeg ua haujlwm tsis raug cai raug txwv tsis pub nkag mus.Qhov chaw huv huv yuav tsum tau ua pa thiab tua kab mob hauv qhov chaw.Cov khoom muaj feem raug muab tso rau hauv ib cheeb tsam, huv si thiab qias neeg raug muab cais tawm, hloov raws sijhawm, thiab decontaminated nyob rau hauv qhov chaw.Cov tshuaj tua kab mob niaj hnub: Cov tshuaj tua kab mob chlorine yog cov tshuaj tseem ceeb rau thaj chaw loj, thiab 75% cawv tuaj yeem siv rau thaj chaw me.Ib txoj hauv kev zoo los daws cov aerosols yog qhib qhov rais rau qhov cua, thiab huab cua tua kab mob kuj tuaj yeem ua los ntawm kev siv hluav taws xob ultraviolet, pom, thiab tshuaj tua kab mob.

Cov kev sib txuas tseem ceeb thiab cov kev ntsuas ntawm SARS-CoV-2 nucleic acid determination (real-time fluorescent RT-PCR)

Txawm hais tias lub chaw soj nstuam feem ntau ua tib zoo saib xyuas cov nucleic acid "nrhiav", qhov tseeb, nucleic acid "extraction" kuj yog ib qho ntawm cov kauj ruam tseem ceeb rau kev tshawb nrhiav kom tiav, uas yog ze ze rau kev sau thiab khaws cov qauv kab mob.

Tam sim no, cov qauv ua pa uas siv dav tshaj plaws, xws li nasopharyngeal swabs, siv txoj kev thib ob, uas yog kev ua kom tsis muaj zog (preservation) tshuaj npaj raws li nucleic acid extraction thiab lysis kua.Ntawm qhov tod tes, cov tshuaj tiv thaiv kab mob no tuaj yeem tiv thaiv cov protein ntawm tus kab mob, poob nws txoj haujlwm thiab tsis kis mus ntxiv, thiab txhim kho kev nyab xeeb ntawm kev thauj mus los thiab kev tshawb pom theem;Ntawm qhov tod tes, nws tuaj yeem cuam tshuam tus kab mob ncaj qha los tso cov nucleic acid, tshem tawm cov nucleic acid decomposing enzyme, thiab tiv thaiv tus kab mob.RNA yog degraded.

Ib qho kab mob sampling tov npaj los ntawm nucleic acid extraction lysis kua.Cov khoom tseem ceeb yog cov ntsev sib npaug, ethylenediaminetetraacetic acid chelating tus neeg sawv cev, guanidine ntsev (guanidine isothiocyanate, guanidine hydrochloride, thiab lwm yam), anionic surfactant (dodecane) Sodium sulfate), cationic surfactant (tetradecyl trimethyl ammonium oxalate 8, hydroxyethyl phenol, phenolase, thiab lwm yam. los yog ntau yam khoom.Tam sim no, muaj ntau hom nucleic acid extraction kits, thiab ntau yam nucleic acid extraction thiab purification reagents yog siv.Txawm hais tias tib lub nucleic acid extraction thiab purification reagent siv, cov txheej txheem rho tawm ntawm txhua cov khoom siv sib txawv.

Tam sim no, cov khoom siv tshuaj ntsuam xyuas nucleic acid tau pom zoo los ntawm National Medical Products Administration raug xaiv raws li ORF1ab, E thiab N noob hauv SARS-CoV-2 genome.Cov qauv kev tshawb pom ntawm cov khoom sib txawv yog qhov zoo ib yam, tab sis lawv cov primers thiab cov qauv kev sojntsuam sib txawv.Muaj qee lub hom phiaj ntu (ORF1ab), ob lub hom phiaj (ORF1ab, N lossis E), thiab peb lub hom phiaj (ORF1ab, N thiab E).Qhov sib txawv ntawm kev tshawb nrhiav thiab kev txhais lus, nucleic acid extraction thiab real-time fluorescent RT-PCR cov tshuaj tiv thaiv kab mob yuav tsum xa mus rau cov lus qhia txog cov khoom siv, thiab nws raug nquahu kom cov neeg siv ua raws li txoj kev txhais lus tau teev tseg hauv cov lus qhia rau kev txhais lus.Cov cheeb tsam sib xws, primers thiab sojntsuam ua ntu zus los ntawm lub sijhawm tiag tiag fluorescent RT-PCR tau qhia hauv P3.

P3 Qhov chaw ntawm SARS-CoV-2 amplicon lub hom phiaj ntawm genome thiab cov kab ke ntawm primers thiab probes

Kev txhais cov txiaj ntsig ntawm SARS-CoV-2 nucleic acid determination (Real-TTom ntej: fluorescent RT-PCR)

"Pneumonia Prevention and Control Plan for SARS-CoV-2 Infection (Second Edition)" thawj zaug tau qhia meej txog cov txheej txheem rau kev txiav txim cov txiaj ntsig ntawm ib qho gene amplification:

1. Tsis muaj Ct lossis Ct≥40 tsis zoo;

2. Ct<37 yog qhov zoo;

3. Tus nqi Ct ntawm 37-40 yog thaj tsam grey.Nws raug pom zoo kom rov ua qhov kev sim.Yog tias qhov tshwm sim ntawm kev rov ua dua Ct <40 thiab qhov kev nthuav dav nkhaus muaj qhov pom tseeb, cov qauv raug txiav txim siab zoo, txwv tsis pub nws yog qhov tsis zoo. "

Daim ntawv thib peb ntawm phau ntawv qhia thiab tsab ntawv thib plaub ntawm phau ntawv qhia txuas ntxiv cov txheej txheem saum toj no.Txawm li cas los xij, vim yog lub hom phiaj sib txawv siv hauv cov khoom siv coj mus muag, cov lus qhia 3rd tsab ntawm cov lus qhia tsis tau muab cov txheej txheem rau kev txiav txim siab ua ke ntawm cov hom phiaj, hais txog cov lus qhia los ntawm cov chaw tsim khoom yuav tsum yeej.Pib los ntawm tsab ntawv thib tsib ntawm cov lus qhia, ob lub hom phiaj tau qhia meej, tshwj xeeb tshaj yog cov qauv txiav txim rau ib lub hom phiaj uas nyuaj rau kev txiav txim.Ntawd yog, yog tias lub chaw kuaj mob xav kom paub tseeb tias qhov xwm txheej zoo rau SARS-CoV-2 nucleic acid kuaj pom, cov hauv qab no yuav tsum tau ua raws li 1 ntawm 2 yam xwm txheej:

(1) Ob lub hom phiaj ntawm SARS-CoV-2 (ORF1ab, N) hauv tib tus qauv raug kuaj pom zoo los ntawm lub sijhawm tiag tiag fluorescent RT-PCR.Yog tias ib lub hom phiaj yog qhov zoo, yuav tsum tau rov kuaj dua thiab rov kuaj dua.Yog tias qhov ntsuas tau yog Yog tias tib lub hom phiaj tseem zoo, nws raug txiav txim tias qhov zoo.

(2) Ob qho piv txwv ntawm lub sijhawm tiag tiag fluorescent RT-PCR tau pom ib lub hom phiaj zoo tib lub sijhawm lossis ob qhov qauv ntawm tib hom tau pom ib lub hom phiaj zoo, uas tuaj yeem txiav txim siab zoo.Txawm li cas los xij, cov lus qhia tseem hais ntxiv tias qhov txiaj ntsig tsis zoo ntawm kev kuaj nucleic acid tsis tuaj yeem tshem tawm tus kab mob SARS-CoV-2.Cov yam ntxwv uas yuav ua rau muaj qhov tsis zoo yuav tsum raug cais tawm, suav nrog cov qauv tsis zoo (cov qauv ua pa los ntawm oropharynx thiab lwm qhov), cov qauv sau ntxov dhau los lossis lig dhau lawm, Cov qauv tsis tau khaws cia, thauj, thiab ua tiav kom raug, thiab thev naus laus zis nws tus kheej muaj teeb meem (tus kab mob sib txawv, PCR inhibition), thiab lwm yam.

Ua rau muaj qhov tsis zoo tsis tseeb hauv kev kuaj pom SARS-CoV-2

Lub tswv yim ntawm "tsis muaj tseeb" hauv kev kuaj nucleic acid uas tam sim no muaj kev txhawj xeeb, feem ntau yog hais txog "tsis muaj qhov tsis zoo" uas cov txiaj ntsig ntawm nucleic acid tsis sib haum nrog cov tsos mob tshwm sim, uas yog, cov tsos mob thiab cov duab pom tau raug xav tias yog COVID-19, tab sis kev kuaj nucleic acid yeej ib txwm "tsis zoo" ntau zaus.Lub Tsev Haujlwm Saib Xyuas Kev Noj Qab Haus Huv Hauv Tebchaws ntawm National Health Commission tau piav qhia qhov "tsis muaj tseeb" SARS-CoV-2 qhov kev sim.

(1) Muaj qee tus kab mob nyob hauv cov hlwb ntawm tus neeg mob.Cov ntaub ntawv uas twb muaj lawm qhia tau hais tias tom qab lub cev kis tus kab mob no, tus kab mob nkag mus rau hauv caj pas los ntawm lub qhov ntswg thiab qhov ncauj, ces mus rau trachea thiab bronchi, thiab ces mus txog lub alveoli.Tus neeg mob yuav ntsib lub sijhawm incubation, cov tsos mob me, thiab tom qab ntawd cov txheej txheem ntawm cov tsos mob hnyav, thiab ntau theem ntawm tus kab mob.Thiab tus nqi ntawm tus kab mob muaj nyob rau hauv ntau qhov chaw ntawm lub cev yog txawv.

Nyob rau hauv cov nqe lus ntawm cov kab mob ntawm cov cell hom, alveolar epithelial hlwb (tso ua pa ib ntsuj av tau)> pais plab epithelial hlwb (sab saum toj ua pa ib ntsuj av tau)> fibroblasts, endothelial hlwb, thiab macrophages, thiab lwm yam.los ntawm hom qauv, alveolar lavage kua (qhov zoo tshaj plaws)> hnoos hnoos sib sib zog nqus> nasopharyngeal swab> oropharyngeal swab> ntshav.Tsis tas li ntawd, tus kab mob no tuaj yeem kuaj pom hauv cov quav.Txawm li cas los xij, xav txog qhov yooj yim ntawm kev ua haujlwm thiab kev lees txais ntawm cov neeg mob, feem ntau siv cov qauv tshuaj ntsuam xyuas yog oropharyngeal swab> nasopharyngeal swab> bronchial lavage kua (kev ua haujlwm nyuaj) thiab cov hnoos qeev tob (feem ntau hnoos qhuav, nyuaj kom tau).

Yog li ntawd, tus nqi ntawm tus kab mob nyob rau hauv lub hlwb ntawm lub oropharynx los yog nasopharynx ntawm ib co neeg mob yog me me los yog tsawg heev.Yog tias tsuas yog cov qauv ntawm oropharynx lossis nasopharynx raug coj mus kuaj, tus kab mob nucleic acid yuav tsis raug kuaj pom.

(2) Tsis muaj cov kab mob uas muaj cov kab mob hauv lub sijhawm sau cov qauv, lossis cov kab mob nucleic acid tsis tau khaws cia zoo.

[① Qhov chaw sau tsis raug, piv txwv li, thaum sau oropharyngeal swabs, qhov tob ntawm kev sau tsis txaus, cov ntaub ntawv sau cov nasopharyngeal tsis tau sau tob rau hauv qhov ntswg kab noj hniav, thiab lwm yam. Feem ntau ntawm cov hlwb uas sau tau tuaj yeem yog cov kab mob tsis muaj kab mob;

②Sampling swabs siv tsis raug.Piv txwv li, hluavtaws fibers xws li PE fiber, polyester fiber thiab polypropylene fiber yog pom zoo rau cov khoom ntawm lub taub hau swab.Ntuj fibers xws li paj rwb yog siv rau hauv kev ua haujlwm tiag tiag (muaj zog adsorption ntawm cov protein thiab tsis yooj yim rau ntxuav tawm) Thiab nylon fibers (kev nqus dej tsis zoo, ua rau tsis txaus sampling ntim);

③ Kev siv cov raj xa dej tsis raug, xws li kev siv tsis raug ntawm polypropylene lossis polyethylene yas khaws cov raj uas yooj yim nqus cov nucleic acids (DNA / RNA), ua rau txo qis ntawm nucleic acid hauv cov tshuaj cia.Hauv kev xyaum, nws raug pom zoo kom siv polyethylene-propylene polymer yas thiab qee qhov tshwj xeeb kho polypropylene yas ntim rau khaws cov kab mob nucleic acids.]

[① Qhov chaw sau tsis raug, piv txwv li, thaum sau oropharyngeal swabs, qhov tob ntawm kev sau tsis txaus, cov ntaub ntawv sau cov nasopharyngeal tsis tau sau tob rau hauv qhov ntswg kab noj hniav, thiab lwm yam. Feem ntau ntawm cov hlwb uas sau tau tuaj yeem yog cov kab mob tsis muaj kab mob;

②Sampling swabs siv tsis raug.Piv txwv li, hluavtaws fibers xws li PE fiber, polyester fiber thiab polypropylene fiber yog pom zoo rau cov khoom ntawm lub taub hau swab.Ntuj fibers xws li paj rwb yog siv rau hauv kev ua haujlwm tiag tiag (muaj zog adsorption ntawm cov protein thiab tsis yooj yim rau ntxuav tawm) Thiab nylon fibers (kev nqus dej tsis zoo, ua rau tsis txaus sampling ntim);

③ Kev siv cov raj xa dej tsis raug, xws li kev siv tsis raug ntawm polypropylene lossis polyethylene yas khaws cov raj uas yooj yim nqus cov nucleic acids (DNA / RNA), ua rau txo qis ntawm nucleic acid hauv cov tshuaj cia.Hauv kev xyaum, nws raug pom zoo kom siv polyethylene-propylene polymer yas thiab qee qhov tshwj xeeb kho polypropylene yas ntim rau khaws cov kab mob nucleic acids.]

(4) Kev ua haujlwm hauv chaw kuaj mob tsis yog tus qauv.Cov qauv kev thauj mus los thiab kev cia khoom, kev ua haujlwm ntawm cov chaw kuaj mob, kev txhais cov txiaj ntsig thiab kev tswj xyuas zoo yog qhov tseem ceeb los xyuas kom meej qhov tseeb thiab kev ntseeg siab ntawm cov txiaj ntsig kev xeem.Raws li cov txiaj ntsig ntawm kev soj ntsuam zoo sab nraud uas tau ua los ntawm Lub Chaw Kho Mob Chaw Kho Mob ntawm National Health Commission thaum Lub Peb Hlis 16-24, 2020, ntawm 844 lub chaw soj nstuam uas tau txais txiaj ntsig zoo, 701 (83.1%) tau tsim nyog, thiab 143 (16.9%) tsis tau.Tsim nyog, tag nrho cov kev kuaj sim hauv chaw kuaj mob yog qhov zoo, tab sis cov chaw soj nstuam sib txawv tseem muaj qhov sib txawv ntawm cov neeg ua haujlwm muaj peev xwm ua haujlwm, ib leeg-lub hom phiaj zoo kev txhais lus muaj peev xwm, thiab kev tswj kom zoo.

Yuav ua li cas txo qhov tsis zoo ntawm SARS-CoV-2 nucleic acid kuaj pom?

Kev txo qis qhov tsis zoo hauv kev tshawb nrhiav nucleic acid yuav tsum tau ua kom zoo dua los ntawm plaub yam ntawm kev tsim cov khoom tsis zoo.

(1) Muaj qee tus kab mob nyob hauv cov hlwb ntawm tus neeg mob.Qhov concentration ntawm tus kab mob nyob rau ntau qhov chaw ntawm lub cev ntawm cov neeg uas xav tias muaj tus kab mob no yuav txawv ntawm lub sijhawm sib txawv.Yog tias tsis muaj pharynx, nws yuav nyob hauv bronchial lavage kua lossis quav.Yog tias muaj ntau hom kev kuaj tuaj yeem sau tau tib lub sijhawm lossis nyob rau theem sib txawv ntawm kev kis kab mob rau kev kuaj, yuav pab kom tsis txhob muaj qhov tsis zoo.

(2) Cov cell uas muaj tus kab mob yuav tsum tau sau thaum lub sij hawm sau cov qauv.Qhov teeb meem no tuaj yeem daws tau rau qhov loj los ntawm kev ntxiv dag zog rau kev cob qhia ntawm cov qauv sau.

(3) Txhim khu kev qha IVD reagents.Los ntawm kev ua cov kev tshawb fawb txog kev soj ntsuam kev ua tau zoo ntawm cov reagents nyob rau hauv lub teb chaws, thiab sib tham txog cov teeb meem uas twb muaj lawm, nrhiav kom tau efficiency ntawm reagents tuaj yeem txhim kho ntxiv thiab rhiab heev ntawm kev tsom xam tuaj yeem txhim kho.

(4) Standardized kev ua haujlwm ntawm cov chaw kuaj mob.Los ntawm kev ntxiv dag zog rau kev cob qhia cov neeg ua haujlwm hauv chav kuaj, tsis tu ncua kev txhim kho lub chaw soj nstuam zoo tswj qhov system, ua kom muaj kev sib faib tsim nyog, thiab txhim kho lub peev xwm ntawm cov neeg ua haujlwm txhawm rau txheeb xyuas, nws muaj peev xwm txo qis qhov tsis zoo vim yog kev ua haujlwm hauv chav kuaj tsis raug.

Yog vim li cas rau qhov rov kuaj pom qhov zoo ntawm SARS-CoV-2 nucleic acid test nyob rau hauv cov neeg mob rov zoo thiab tso tawm

"COVID-19 Kev Tshawb Fawb thiab Kev Npaj Kho Mob (Tshwj Sim Xya Hli)" qhia meej tias ib qho ntawm cov txheej txheem rau cov neeg mob COVID-19 yuav tsum tau kho thiab tawm hauv tsev kho mob yog tias ob qhov kev kuaj ua pa sib txuas ua rau muaj qhov tsis zoo nucleic acid kuaj (tsawg kawg 24 teev sib nrug), tab sis muaj tsawg heev vim li cas cov neeg mob SARS-CoV-2 tawm ntau qhov laj thawj.

(1) SARS-CoV-2 is a new virus.Nws yog ib qho tsim nyog kom nkag siab ntxiv txog nws cov txheej txheem pathogenic, tag nrho cov duab ntawm tus kab mob tshwm sim thiab cov yam ntxwv ntawm cov kab mob.Yog li ntawd, ntawm ib sab, nws yog ib qho tsim nyog los ntxiv dag zog rau kev tswj hwm cov neeg mob tawm thiab ua 14-hnub kev soj ntsuam kev kho mob.Ua raws li kev soj ntsuam, kev saib xyuas kev noj qab haus huv thiab kev coj noj coj ua kom nkag siab tob txog tag nrho cov txheej txheem ntawm qhov tshwm sim, kev loj hlob thiab qhov tshwm sim ntawm tus kab mob.

(2) Tus neeg mob yuav kis tus kab mob ntxiv.Tus kws tshaj lij Zhong Nanshan tau hais tias: Vim tias cov neeg mob kho tau muaj cov tshuaj tiv thaiv kab mob, SARS-CoV-2 tuaj yeem raug tshem tawm los ntawm cov tshuaj tiv thaiv thaum lawv rov ua dua.Muaj ntau qhov laj thawj, uas tej zaum yuav yog qhov ua rau tus neeg mob rov zoo, lossis nws yuav muaj feem cuam tshuam nrog kev hloov pauv ntawm tus kab mob, lossis txawm tias ua rau muaj kev sim kuaj.Yog tias nws yog tus kab mob nws tus kheej, SARS-CoV-2 kev hloov pauv tuaj yeem ua rau cov tshuaj tiv thaiv uas tsim los ntawm tus neeg mob rov qab los ua tsis tau zoo tiv thaiv tus kab mob hloov pauv.Yog tias tus neeg mob tau kis tus kab mob hloov pauv dua, qhov kev kuaj nucleic acid yuav zoo dua.

(3) Raws li kev soj ntsuam kev soj ntsuam muaj kev txhawj xeeb, txhua txoj kev sim muaj nws cov kev txwv.SARS-CoV-2 nucleic acid detection yog vim qhov kev xaiv ntawm cov noob caj noob ces, muaj pes tsawg leeg ntawm reagents, rhiab heev ntawm txoj kev thiab lwm yam laj thawj, ua rau cov khoom siv uas twb muaj lawm muaj lawv cov kev txwv qis dua.Tom qab tus neeg mob tau kho, tus kab mob hauv lub cev txo qis.Thaum cov kab mob kis tau rau hauv cov qauv uas yuav tsum tau kuaj yog qis dua qhov kev kuaj pom, qhov tshwm sim "tsis zoo" yuav tshwm sim.Txawm li cas los xij, qhov tshwm sim no tsis tau txhais hais tias tus kab mob hauv lub cev tau ploj mus tag nrho.Tus kab mob no tuaj yeem yog tom qab kev kho mob raug tso tseg.Resurgence", txuas ntxiv mus luam.Yog li ntawd, nws raug nquahu kom tshuaj xyuas ib zaug ib lub lim tiam hauv 2 mus rau 4 lub lis piam tom qab tso tawm.

(4) Nucleic acid yog cov khoom siv caj ces ntawm tus kab mob.Tus kab mob no raug tua tom qab tus neeg mob tau txais kev kho mob antiviral, tab sis cov seem ntawm cov kab mob RNA tseem khaws cia hauv tib neeg lub cev, thiab tsis tawm hauv lub cev tag nrho.Qee zaum, nyob rau qee qhov xwm txheej, nws tuaj yeem khaws cia ntau dua.Lub sijhawm ntev, thiab lub sijhawm no qhov kev sim nucleic acid yuav "ntev" zoo.Nrog rau qhov txuas ntxiv ntawm tus neeg mob lub sijhawm rov qab los, tom qab cov seem RNA seem hauv lub cev tau maj mam tag, cov txiaj ntsig nucleic acid tuaj yeem ua rau tsis zoo.

(5) Cov txiaj ntsig ntawm nucleic acid test ntawm SARS-CoV-2 tsuas yog ua pov thawj tias muaj lossis tsis muaj tus kabmob RNA, thiab tsis tuaj yeem ua pov thawj cov haujlwm ntawm tus kabmob thiab seb tus kabmob puas kis tau.Nws yog ib qho tsim nyog los ua pov thawj seb tus neeg mob uas tau kuaj pom zoo nucleic acid dua puas yuav dhau los ua tus kab mob ntxiv.Nws yog ib qho tsim nyog los ua cov kab lis kev cai ntawm tus kab mob ntawm cov qauv kuaj mob thiab cog cov kab mob "nyob" los ua pov thawj tias nws kis tau.

Cov ntsiab lus

Hauv cov ntsiab lus, SARS-CoV-2 nucleic acid kuaj qhov tsis zoo tsis zoo, rov kuaj qhov zoo, thiab lwm yam xwm txheej uas tsis sib haum nrog kev kho mob tshwm sim tsis tuaj yeem zam tag nrho.Hauv kev tshuaj ntsuam xyuas thiab kev sim tiag tiag, nws raug pom zoo kom muab cov tsos mob, kev kuaj xyuas (CT) thiab kev sim Laboratory test (nucleic acid test + virus-specific antibody test) cov txiaj ntsig rau kev kuaj mob dav dav kom tiv thaiv tsis tau kuaj mob thiab kuaj tsis raug.Yog tias qhov ntsuas tau pom tias pom tau tias tsis sib haum nrog cov tsos mob tshwm sim, nws raug nquahu kom ua qhov kev tshuaj ntsuam xyuas ntawm tag nrho cov kev sib txuas (cov qauv sau, kev ncig thiab kev sib txuas ua haujlwm) kom tsis suav nrog tus kab mob SARS-CoV-2 thaum ntxov, kab mob rov tshwm sim lossis ua ke nrog lwm yam kab mob ua pa, thiab lwm yam.Yog tias muaj kev tso cai, nws raug pom zoo kom sau cov qauv rhiab ntxiv xws li hnoos qeev lossis alveolar lavage kua rau rov kuaj dua.

Yam khoom:

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)