Direct PCR
Direct PCR yog dab tsi
Direct PCR yog txoj kev ntawm DNA amplification ncaj qha los ntawmntshav, tsiaj cov ntaub so ntswg,rat qab,cell, pob ntseg,zebra ntses, ntses qe,nplooj nplooj,nooblos yog lwm yam qauv ntawm cov ntaub so ntswg uas tsis muaj DNA cais thiab ua kom huv si.Cov txheej txheem PCR ncaj qha tuaj yeem txo lub sijhawm sim, thiab tus nqi hauv genotyping thiab ntau qhov project.Nws kuj tseem muab cov kev xaiv zoo dua thaum ntsib cov teeb meem ntawm kev nthuav dav me me ntawm cov qauv uas cov kauj ruam ua kom huv tuaj yeem ua rau cov qauv poob.
FOREGENE's PCR Mix muaj zog tiv thaiv kev ntxhov siab, vim FOREGENE lub zog patented Taq enzyme (tso cai patent: ZL20161034512.0 (Tuam Tshoj), EP3450560 (Tebchaws Europe), PCT/CN2017/082154 (US), patent 68Jap49).Nws Taq enzyme siv DNA recombination thev naus laus zis los ua kom cov DNA khi cov qauv hauv cheeb tsam ntawm archaeal nucleic acid binding protein nrog cov tshav kub-resistant Taq DNA polymerase los tsim cov qauv DNA nrog kev sib txuas ntxiv.Ib qho kev txhim kho kub-pib Taq uas khaws ntau yam haujlwm ntawm thawj DNA polymerase.This Taq enzyme tuaj yeem khi rau cov qauv DNA thiab pib DNA synthesis hauv cov qauv qis qis thiab cov cheeb tsam nyuaj.
For piv txwv,ityog yooj yim li ntxiv kojcell,nroj tsuag (cov nplooj hluas, cag los yog noob) los yog tsiaj coj mus rau lubtsis, thiab ntxiv qhov tshwj xeebmaster mix nrogtshwj xeebprimers nyob rau hauv PCR raj thiab khiav nws los ntawm ib tug thermal cycler.Cov khoom siv tau zoo yog qhov zoo tagnrho rau cov qauv ntsuas loj uas txuag lub sijhawm tseem ceeb heev.
PCR cov tshuaj tiv thaiv uas muaj UNG enzyme thiab dUTP raug xaiv raws li cov kev xav tau ntawm kev sim, uas tuaj yeem zam tau cov pa phem los ntawm PCR amplification khoom.
Qhov zoo ntawmDirect PCR
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| Direct PCR | Kev lig kev cai (RNA purification + Coventional PCR) |
Khoom siv | Cov ntaub so ntswg noj | Tsawg dua | Ntau |
Kev npaj template | Hom khoom | Tsis xav tau kev sib tsoo | Sib tsoo cov qauv |
Kev ua haujlwm | Ib-tube hom, 10 min | kev ua haujlwm nyuaj, 1h | |
Flux | 1-96 | 1-24 | |
Cov tshuaj tiv thaiv PCR | tshuaj tiv thaiv kab mob | Optimized 2 × PCR mix | Dntp, MgCl2, 10 × PCR Buffer, Taq enzyme, |
Adaim ntawv thov
01 Kev txheeb xyuas cov kab mob pathogenic
02 Genetically hloov kev txheeb xyuas, genotyping
03 Multiplex PCR / SNP nrhiav tau / PCR-RFLP
04 Sequencing / cloning
Khoom Series--Plant Direct PCR Series
Series | Khoom npe | Specifications | Catalog Number | Kev cia khoom |
Cog Direct PCR Series | 200 × 20μl rxns | TSO-02111 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-02113 | |||
200 × 20μl rxns | TSI-02121 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-02123 | |||
Plant Leaf Direct PCR Plus Kit | 200 × 20μl rxns | TSI-02131 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-02133 | |||
Plant Leaf Direct PCR Plus Kit -UNG | 200 × 20μl rxns | TSI-02141 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-02143 | |||
200 × 20μl rxns | TSI-03111 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-03113 | |||
200 × 20μl rxns | TSI-03121 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-03123 | |||
200 × 20μl rxns | TSI-03131 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-03133 | |||
200 × 20μl rxns | TSI-03141 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-03143 | |||
Plant Seed Direct PCR Plus Kit I (Polysaccharide polyphenol cog) | 200 × 20μl rxns | TSI-03151 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-03153 | |||
Plant Seed Direct PCR Plus Kit I - UNG (Polysaccharide polyphenol cog) | 200 × 20μl rxns | TSI-03161 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-03163 | |||
200 × 20μl rxns | TSI-03171 | Ntu I 4 ℃, Ntu II -20 ℃ | ||
2000 × 20μl rxns | TSI-03173 | |||
Plant Seed Direct PCR Plus Kit II - UNG (Polysaccharide polyphenol cog) | 200 × 20μl rxns | TSI-03181 | Ntu I 4 ℃, Ntu II -20 ℃ | |
2000 × 20μl rxns | TSI-03183 |