PCR txoj kev thiab nucleic acid aerosol paug nyob rau hauv nucleic acid kuaj kuaj yog zoo li ob sab ntawm ib npib.Peb tsuas tuaj yeem xaiv kom muaj lossis tsis muaj, tab sis peb tsis tuaj yeem xaiv seb peb xav tau lossis siv nws.

1. Kev tshuaj ntsuam DNA tshem tawm

Yuav kom ua tiav qhov kev tshem tawm ntawm nucleic acid aerosol paug, nws yog thawj zaug uas yuav tsum tau tshuaj xyuas cov DNA tshem tawm uas tuaj yeem tshem tawm cov nucleic acids hauv lub xeev ua kua.Vim tias tsis muaj ntau cov DNA tshem tawm uas ua haujlwm tiag tiag.Rau txoj kev sim, thov xa mus rau: DNA tshem tawm tsis tuaj yeem yog "lub ces kaum zais cia" ntawm chav kuaj!

Hauv qhov kev sim no, 100 daim ntawv luam / μL (CT kwv yees li 31) ntawm ASFV plasmid thiab DNA tshem tawm kom muaj nuj nqis los ntawm cov PCR raug muab sib xyaw rau hauv qhov sib npaug, thiab tom qab ntawd ua rau hauv chav sov li 10min, 20min thiab 30min feem.Tom qab nucleic acid extraction, qPCR amplification tau nqa tawm.Ib qho kev tswj zoo sib xyaw nrog plasmid thiab dej tau muab piv.Txij li qhov kev sim tsis ua tiav tib lub sijhawm, tej zaum yuav muaj qee qhov sib txawv ntawm cov txiaj ntsig, tab sis nws tsis cuam tshuam rau qhov xaus ntawm qhov kev sim.Txog tam sim no, kuv tau soj ntsuam 10 cov khoom lag luam DNA tshem tawm.Tsuas yog No. 1, No. 6 thiab No. 8 cov khoom tuaj yeem degrade plasmid DNA zoo hauv lub xeev ua kua.Lwm cov khoom yuav luag tsis muaj txiaj ntsig.

Table 1 Nucleic acid tshem tawm cov txiaj ntsig ntawm kev lag luam muaj DNA tshem tawm

2. Cov txiaj ntsig DNA tshem tawm kev sim ntawm cov tshuaj tua kab mob uas nquag siv

1. Kev tshuaj xyuas cov tshuaj tua kab mob uas nquag siv

Muaj ntau hom tshuaj tua kab mob uas nquag siv hauv chav kuaj: aldehydes, phenols, cawv, quaternary ammonium ntsev, peroxides, chlorine npaj, thiab cov kua qaub thiab cov hauv paus.Cov tshuaj tua kab mob los ntawm cov tshuaj tua kab mob ntawm cov kab mob no tau raug tshawb xyuas tag nrho los ntawm kev sim, tab sis tsis muaj cov ntaub ntawv sim txaus ntawm cov nyhuv degradation ntawm nucleic acids.Nucleic acid kuaj cov chaw kuaj mob xav tau cov tshuaj tua kab mob uas tsis yog tsuas yog muaj cov tshuaj tua kab mob zoo rau cov kab mob, tab sis kuj tuaj yeem degrade DNA.Cov txiaj ntsig kev sim qhia tau hais tias tsuas yog ob qho tshuaj chlorine ntawm 84 tshuaj tua kab mob thiab trichloroisocyanuric acid thiab 1 M hydrochloric acid ua tau raws li qhov yuav tsum tau ua.Txij li thaum 1 M hydrochloric acid yog corrosive heev, nws raug nquahu kom siv cov tshuaj chlorine los ua pov tseg hauv cov chaw kuaj nucleic acid.Txawm li cas los xij, kev npaj tshuaj chlorine yog corrosive rau hlau thiab tsis tuaj yeem siv rau kev tua kab mob ntawm cov cuab yeej thiab khoom siv.

Table 2 DNA tshem tawm cov teebmeem ntawm ntau hom tshuaj tua kab mob

2. Qhov tsawg kawg nkaus ua tau zoo ntawm cov tshuaj chlorine npaj

Cov tshuaj tua kab mob chlorine muaj cov txiaj ntsig zoo DNA degradation, tab sis vim lawv cov hlau corrosiveness thiab khaus khaus, lawv tuaj yeem siv los ntxuav hauv pem teb, cov txee uas tsis yog hlau, cov lus qhia so, cov raj centrifuge thiab lwm yam khoom kuaj.

Raws li "Tshiab Coronary Pneumonia Epidemic Disinfection Technical Guidelines": Cov pa phem (cov neeg mob cov ntshav, zais zis thiab ntuav) muaj cov tshuaj chlorine 5g/L-10g/L chlorine-muaj tshuaj tua kab mob;cov plag tsev, phab ntsa, thiab cov khoom nto siv cov tshuaj chlorine 1g/L chlorine tshuaj tua kab mob: khaub ncaws, ntaub pua chaw pw thiab lwm yam textiles yog thawj zaug soaked nyob rau hauv tshuaj tua kab mob chlorine nrog 0.5g/L muaj chlorine rau 30 feeb, thiab ces ntxuav li qub.

Table 3 DNA tshem tawm cov txiaj ntsig ntawm qhov sib txawv ntawm cov tshuaj tua kab mob chlorine

Cov txiaj ntsig kev sim qhia tau hais tias: thaum cov tshuaj chlorine muaj txiaj ntsig zoo dua lossis sib npaug ntawm 1.2 g / L, cov tshuaj tua kab mob chlorine tuaj yeem ua rau 100 daim ntawv / μL plasmid tag nrho thaum nws siv 5 feeb.Thaum ua yeeb yam rau 10 feeb, 84 tshuaj tua kab mob uas muaj cov tshuaj chlorine muaj txiaj ntsig ntau dua lossis sib npaug ntawm 0.6 g / L tuaj yeem degrade 100 daim ntawv / μL ntawm plasmids.

3. Kev sim tshem tawm cov pa phem los ntawm nucleic acid aerosol

Yuav ua li cas ntxuav cov pa phem los ntawm nucleic acid aerosols?Feem ntau ntawm cov kev xav yog tsim kom muaj ib qho chaw uas muaj kab mob aerosol thiab tom qab ntawd ntxuav nws, lossis ntxuav cov nucleic acids tom qab kis kab mob.Rau kuv, qhov kev sim uas tsis tuaj yeem rov ua dua thiab cov txiaj ntsig ntawm qhov kev sim tsis tuaj yeem txheeb xyuas ntau yam tsis muaj qab hau, yog li kuv tau qiv qee txoj hauv kev tshuaj tua kab mob hauv huab cua.Saib "GB27948-2020 General Requirements for Air Disinfectants" thiab "Disinfection Technical Specifications"

1. Cov ntaub ntawv xeem

1.1 DNA tshem tawm: Hauv qhov kev sim no, ob qho kev lag luam DNA tshem tawm 6 thiab 8 hauv Table 1 raug xaiv.

1.2 Test plasmid: Xav txog tias CT tus nqi ntawm cov pa phem feem ntau yuav siab dua 30, qhov concentration ntawm ASFV noob plasmid siv lub sijhawm no yog kwv yees li 100 daim ntawv / μL, thiab cov fluorescent quantitative PCR CT yog li 31.07.Ntxiv cov plasmid rau cov paj rwb swab thiab cua qhuav.

1.3 Kev ntsuas ntsuas: Cov cuab yeej tshuaj tua kab mob Bilingkehan KVBOX, uas tau txais txiaj ntsig zoo los ntawm Shenzhen Runlian Environmental Technology Co., Ltd., muaj cov tshuaj tua kab mob zoo heev nrog cov tshuaj tua kab mob hydrogen peroxide, thiab tuaj yeem tua cov kab mob thiab lwm yam kab mob.

1.4 Qhov chaw kuaj: kaw qhov rais hloov chaw ntawm 0.1 cubic meters.

2. Kev sim

Tus nqi ntawm cov tshuaj tua kab mob tso rau ntawm lub tshuab tua kab mob yog 10ml, thiab qhov concentration yog 100ml / m3, uas yog ntau dua li "GB27948-2020 General Requirements for Air Disinfectants": thaum siv cov tshuaj tua kab mob aerosol, cov tshuaj tua kab mob yuav tsum yog ≤10 ml / m3.