Koj paub ntau npaum li cas

Txog Nextraction ntawm ukleic acid

Qhov pib thiab qhov kawg ntawm purified nucleic acid

Txhua yam nyuaj thaum pib, purified nucleic acid yog qhov feem ntau

Pib ntawm kev sim molecular, rau kev sim tom ntej

Kev vam meej lossis tsis ua tiav muaj qhov cuam tshuam tseem ceeb.

Trace / ultra-trace UV spectrophotometer nyiam los ntawm ntau cov kws tshawb fawb tshawb fawb vim tias nws tuaj yeem ntes nucleic acid concentration thiab protein thiab ntsev ion residues yooj yim, sai thiab kev lag luam.

Raws li peb txhua tus paub, A260 txhais tau tias nucleic acid absorbance OD tus nqi, A280 txhais tau hais tias protein concentration absorbance OD tus nqi, A230 txhais tau tias ntsev ion concentration absorbance OD tus nqi, yog li A260 tuaj yeem hloov cov nucleic acid concentration, A260/280 txhais tau tias muaj protein ntau, A260/230 txhais tau tias ntsev ion residue, tab sis qhov no tsuas yog ib txoj cai los ntawm kev tshawb fawb loj.ib txwm"kom ntseeg tau tias nws tuaj yeem piav qhia qhov purity ntawm DNA thiab RNA rauib qho twg.Nws tsis yog tus qauv nkaus xwb rau kev txheeb xyuas cov txiaj ntsig nucleic acid thiab purity, nws tsuas yog siv los ua kev siv, thiab nws tsis yog "tus qauv kub".

Phau ntawv Thermo Fisher ND-2000 qhia txog kev ntseeg siab ntawm cov txiaj ntsig kev xeem

Yog vim li cas yog tias cov txiaj ntsig tau los ntawm kev siv micro / ultramicro UV spectrophotometer tsuas yog cuam tshuam cov txiaj ntsig thiab purity ntawm nucleic acid los ntawm sab, thiab muaj ntau yam cuam tshuam (optical path, qauv ntim, qauv concentration, pH tus nqi, lwm yam ions uas cuam tshuam rau kev ntsuas absorbance, thiab lwm yam), qhov ntsuas OD tsis yog qhov tseeb.Nyob rau tib lub sijhawm, vim qhov tsis muaj qhov tshwj xeeb ntawm qhov kev txiav txim siab tus nqi absorbance khoom, ib leeg-stranded DNA, ob-stranded DNA, RNA, dNTPs, thiab qee cov proteins tag nrho muaj qhov nqus siab siab ntawm 260nm wavelength, thiab qhov concentration txiav txim los ntawm A260 ib leeg tsis tas yuav yog DNA lossis RNA tiag.Concentration, thiab nws txoj kev ncaj ncees thiab degradation yuav tsis raug txiav txim.

Yog li yuav ua li cas txiav txim qhov zoo ntawm peb cov purified nucleic acid?Ntxiv nrog rau kev siv lub micro/ultramicro UV spectrophotometer rau kev kuaj pom, txoj hauv kev xws li agarose gel electrophoresis kuj tseem yuav tsum tau ua kom pom qhov purity thiab kev ncaj ncees ntawm nucleic acid, thiab qhia cov concentration rau qee yam.Nyob rau hauv txoj kev no, cov nucleic acid tawm los thiab purity tau los ntawm kev tshawb pom cov txiaj ntsig ntawm ntau txoj hauv kev tau ntseeg tau.

Kev sim daim duab sib piv

Genomic DNA ntawm H1299 hlwb tau muab rho tawm siv lub tuam txhab A's DNA Extraction cov khoom siv, thiab pom muaj qhov tsis zoo ntawm qhov tsis huv, ua rau muaj txiaj ntsig OD siab.

(OD ntsuas tus nqi thiab cov khoom siv electropherogram)

Kev ntsuas ntsuas

Puas muaj "tus qauv kub" rau nucleic acid tawm los thiab kev sim zoo?

Raws li peb paub, tsis muaj txoj hauv kev yooj yim, nrawm thiab pheej yig.Txawm hais tias nws yog spectrophotometer, gel electrophoresis, los yog huab hwm coj spectrometry, lawv txhua tus muaj kev cuam tshuam cov concentration thiab purity ntawm nucleic acid nyob rau hauv cov tshuaj los ntawm ntau yam, thiab lawv yuav tsum tau txiav txim los ntawm kev xa mus rau ib leeg.

Qhov ntsuas ntsuas zoo tshaj plaws yog ib txwmdaim ntawv thov kev soj ntsuam tom qab.Nws tsis cuam tshuam qhov kev ua tau zoo ntawm kev thim rov qab thiab PCR amplification.Tau txais cov khoom lag luam txhim khu kev qha thiab Ct qhov tseem ceeb, thiab tau txais ib qho kev ua tiav los ntawm kev sib tw yog kev ua tiav nucleic acid extraction.

Txhawm rau ua kom tiav, qhov kev xav ntawm qhov piv txwv nkaus xwb yuav tsum tau sib tw los ntawm qhov kev pom ntawm "siv cov txiaj ntsig zoo hauv qab qhov kev sim ua qhov zoo rho tawm"!

Pom zoo Foregene DNA RNA cov khoom siv rho tawm kom tau txais DNA / RNA purity siab:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/