lncRNA nta:

1. lncRNAs feem ntau yog ntev dua, nrog dynamic qhia thiab txawv splicing txoj kev thaum sib txawv;

2. Piv nrog cov noob caj noob ces, lncRNA feem ntau qis dua;

3. Feem ntau lncRNAs muaj qhov pom tseeb ntawm sab cev nqaij daim tawv thiab spatial qhia tshwj xeeb hauv cov txheej txheem ntawm cov ntaub so ntswg sib txawv thiab kev loj hlob;

4. Muaj cov yam ntxwv ntawm cov qog thiab lwm yam kab mob;

5. Qhov chaw subcellular ntawm lncRNA muaj ntau haiv neeg;

6. Cov kab ke tsis tshua muaj kev txuag, tab sis kev ua haujlwm muaj qee qhov kev txuag thiab lwm yam.

LncRNA thim rov qab kev daws teeb meem:

Peb paub tias lncRNA muaj cov qauv qib siab hauv cov hlwb.Xws li RNA feem ntau muaj cov qauv tsim xws li qia loops lossis hairpins.Yog tias tus qauv thib ob ntawm lncRNA tsis tuaj yeem daws tau zoo, rov qab cov ntaub ntawv rov qab yog qhov ua tsis tiav lossis tsis ua tiav thaum ua RT-qPCR.Qhov teeb meem ntawm kev ua haujlwm tsawg.

Feem ntau, muaj peb lub ntsiab lus tseem ceeb rau peb kom tau txais cov qauv zoo cDNA:

RNA template zoo, rov qab transcription primers thiab thim rov qab transcriptase, thiab thim rov qab transcriptase yog qhov tseem ceeb tshaj plaws.RNA purified los ntawm feem ntau siv RNA extraction reagents feem ntau muaj residues ntawm cawv thiab guanidine ntsev, uas muaj zog inhibitory cuam tshuam rau feem ntau reverse transcriptases;Yog tias qhov ntsuas kub zoo tshaj plaws rau qhov rov qab transcriptase cov tshuaj tiv thaiv qis, nws yuav tsis zoo rau RNA.Qhov qhib ntawm cov qauv hierarchical.

Tam sim no, qhov ntsuas kub tsim nyog rau kev rov qab transcriptase siv nyob rau hauv ntau lub chaw soj nstuam yog feem ntau 42 ° C, thiab qhov kub siab tshaj plaws tuaj yeem ncav cuag li 50 ° C.Qhov tseeb, qhib qhov kev txiav txim siab dua RNA qauv yuav tsum muaj qhov kub siab dua.

Qhov kub rau kev sib xyaw ua ke ntawm thawj-strand cDNA ntawm Fortune Biotech 2 × RT EasyTM Mix yog siab li 50 ℃ -65 ℃, uas yog qhov tsim nyog rau kev tshem tawm cov qauv qib siab ntawm lncRNA, thiab lub sijhawm ua haujlwm luv luv.

Tsis tas li ntawd, cov khoom lag luam kuj muaj peev xwm tiv thaiv kev cuam tshuam rau RNA nrog residual cawv thiab guanidine ntsev, uas tuaj yeem ua tau raws li qhov xav tau ntawm lub chaw soj nstuam kom ua tiav qhov kev rov qab ua haujlwm siab.

Thawj Strand cDNA Synthesis Kit

Yam Tsis Tau: RT-01011 (25 zaug)

Nta:

1. Thermal stability: 65 ℃ tseem muaj kev ua haujlwm rov qab zoo, uas pab qhib cov txheej txheem theem nrab.

2. Kev kam rau siab: cDNA synthesis tseem tuaj yeem ua tiav ntawm RNA templates nrog tsis zoo purity (ethanol thiab guanidine ntsev residues)

3. Ceev: kev ua haujlwm yooj yim, thawj strand cDNA synthesis tuaj yeem ua tiav hauv 25-35 feeb

Cov ntawv thov hauv qab: PCR, qPCR, RACE, chip labeling, tsev qiv ntawv tsim, thiab lwm yam.

Tsis tas li ntawd, FJ Biotech muab cov txheej txheem tag nrho ntawm RT-PCR cov kev daws teeb meem kom tau raws li qhov xav tau ntawm cov neeg siv khoom sib txawv, suav nrog cov khoom siv hauv qab no:

Thawj Strand cDNA Synthesis Kit (Rau qPCR)

Yam Tsis Tau: RT-01021 (50 zaug)

Nta:

1. Yooj yim: Rov qab cov ntaub ntawv rov qab tau muab ntxiv kom ua haujlwm yooj yim dua

2. Thermal stability: 65 ℃ tseem muaj kev ua haujlwm rov qab zoo, uas pab qhib cov txheej txheem theem nrab.

3. Kev kam rau siab: cDNA synthesis tseem tuaj yeem ua tiav ntawm RNA templates nrog tsis zoo purity

4. Sensitivity: haum rau tsawg-concentration RNA qauv

Daim ntawv thov hauv qab: tshwj xeeb yog muab rau qPCR tom ntej

Ib-kauj ruam RT-PCR cov khoom siv

Yam Tsis Tau: RT-02011 (100 zaug)

Nta:

1. Thermal stability: 65 ℃ tseem muaj kev ua haujlwm rov qab zoo, uas pab qhib cov txheej txheem theem nrab.

2. Kev kam rau siab: cDNA synthesis tseem tuaj yeem ua tiav ntawm RNA templates nrog tsis zoo purity

3. Specificity: Lub nruab PCR MIX zoo txhim kho lub amplification efficiency thiab tshwj xeeb ntawm cov tshuaj tiv thaiv.

Cov ntawv thov hauv qab: cDNA cloning, kuaj caj ces thiab lwm yam kev sim molecular

Ob-kauj ruam RT-PCR cov khoom siv

Yam Tsis Tau: RT-02021 (100 zaug)

Nta:

1. Yooj yim thiab ceev: ib lub raj tshuaj tiv thaiv

2. Thermal stability: 65 ℃ tseem muaj kev ua haujlwm rov qab zoo, uas pab qhib cov txheej txheem theem nrab.

3. Specificity: Qhov ntxiv Foregene Taq zoo txhim kho qhov ua kom muaj zog thiab qhov tshwj xeeb ntawm cov tshuaj tiv thaiv

Cov ntawv thov hauv qab: kev sim tshuaj molecular xws li kev kuaj caj ces

Ib-kauj ruam RT-qPCR cov khoom siv-SYBR Green I

Yam Tsis Tau: RT-02111 (100 zaug)

Nta:

1. Yooj yim: ib lub raj tshuaj tiv thaiv

2. Siab rhiab heev: tuaj yeem ntes cov qauv luam tawm tsawg

3. Compatibility: haum rau feem ntau fluorescence quantitative instruments

Cov ntawv thov hauv qab: tshawb nrhiav cov kab RNA cov qauv xws li cov hlwb thiab cov kab mob

Ib-kauj ruam RT-qPCR cov khoom siv-Taqman

Yam Tsis Tau: RT-02131 (100 zaug)

Nta:

1. Ceev ceev: kuaj lub hom phiaj gene

2. Siab rhiab heev: tuaj yeem ntes cov qauv luam tawm tsawg

3. Compatibility: haum rau feem ntau fluorescence quantitative instruments

Cov ntawv thov hauv qab: tshawb nrhiav cov kab RNA cov qauv xws li cov hlwb thiab cov kab mob
Forge Bioreverse Transcription Kit tuaj yeem pab koj daws qhov teeb meem ntawm kev thim rov qab.Nyob rau tib lub sijhawm, nws koom tes nrog Forge Bio RNA Extraction Kit (uas tuaj yeem tshem tawm DNA seem zoo) los muab koj cov kev sim ua tiav RNA.