Kub-pib Taq enzyme yog dav siv.Piv nrog rau DNA polymerase zoo tib yam, kub-pib Taq enzyme tuaj yeem tiv thaiv qee qhov tsis tshwj xeeb amplification thiab tsim cov primer dimers, thiab tuaj yeem txhim kho qhov kev vam meej ntawm lub hom phiaj gene amplification.Tshwj xeeb tshaj yog nyob rau hauv kev ntsuam xyuas caj ces, kub pib Taq enzyme tau raug txheeb xyuas raws li tus qauv yuav tsum tau ua hauv kev lag luam, thiab cov DNA polymerase zoo tib yam yuav tsum tsis txhob siv.Raws li tau pom los ntawm cov saum toj no, kub pib Taq enzymes tau dav siv.Tam sim no, muaj ntau lub npe kub pib Taq enzymes nyob rau hauv lub lag luam, tab sis tsis muaj ntau kub pib Taq enzymes uas zoo.Tau ntsib nrog ntau cov khoom kub pib Taq enzyme, peb yuav xaiv li cas?

1. Xaiv qhov kub-pib Taq enzyme nrog kev ua haujlwm siab amplification

PCR amplification efficiency yog ze ze rau kev ua haujlwm ntawm Taq enzyme.Tom qab qhov zoo ntawm Taq enzyme cov tshuaj tiv thaiv yog optimized, lub amplification efficiency yog siab tshaj 95%, thiab lub amplification ntau yam ntawm tus thawj template tus nqi yog dav.Txaus siab rau amplification tuaj yeem tau txais thaum lub hom phiaj gene cov ntsiab lus qis, thiab nws tsis yooj yim rau kev lom thaum tus nqi template siab, thiab lub sij hawm exponential amplification ntev.Rau Taq enzyme nrog kev ua haujlwm tsis zoo, txawm tias cov tshuaj tiv thaiv tau ua kom zoo rau ntau lub sijhawm, qhov kev ua kom zoo dua qub tseem tsawg dua 90%, qhov "S" zoo ntawm qhov kev nthuav dav nkhaus tsis pom tseeb, txoj kab nqes me me, thiab qhov nkhaus yog tiaj tus.Thaum tus nqi ntawm cov qauv qis, nws tsis tuaj yeem nthuav dav, thiab thaum tus nqi ntawm cov qauv siab, cov nyhuv amplification tsis zoo tagnrho.Yog li ntawd, kev xaiv ntawm DNA polymerases nrog kev ua haujlwm siab amplification yog qhov tseem ceeb rau kev ua tiav ntawm PCR thiab qPCR.

2. Xaiv kub pib Taq enzyme nrog lub zog enzyme muaj zog

Lub zog enzymatic ntawm Taq enzyme muaj feem xyuam rau kev ua kom muaj zog.Feem ntau, lub zog enzymatic ntawm qhov kub-pib Taq enzyme, ntev lub sij hawm exponential kev loj hlob ntawm PCR amplification, ntau dua 'S-shaped' nkhaus, qhov siab dua cov teeb liab fluorescence, thiab qhov ntau haum rau multiplex PCR nrhiav tau.Hom DNA polymerases nrog lub zog tsis muaj zog enzymatic feem ntau tsuas yog txhawb nqa 2-plex cov tshuaj tiv thaiv.Thaum ua 3-plex cov tshuaj tiv thaiv, lub amplification nkhaus yog tsawg, fluorescence teeb liab tus nqi yog tsawg, thiab tsis muaj ib tug raug amplification nkhaus, yog li ntawd cov kev tshwm sim yog nyuaj rau txiav txim.

3. Xaiv qhov kub pib Taq enzyme nrog siab rhiab heev

Feem ntau hais lus, DNA polymerase muaj qhov ua tau zoo ntawm kev ua haujlwm siab thiab siab rhiab heev, tab sis kuj tseem muaj qhov tsis sib xws.Yog hais tias lub hom phiaj gene abundance ntawm cov qauv yuav tsum tau amplified yog tsawg, nws raug nquahu kom sim cov amplification rhiab heev ntawm Taq enzyme.Txoj kev kuaj pom ntau tshaj plaws yog ua kom tiav 10-fold lossis 5-fold gradient dilution ntawm lub hom phiaj noob plasmid fragment, nqa tawm PCR nrhiav tau ntawm qhov qis dilution, thiab xaiv qhov kub-pib Taq enzyme nrog ntau dua kev tshawb nrhiav rhiab heev.

Nws tuaj yeem pom los ntawm cov saum toj no uas cov kws tshawb fawb yuav tsum xaiv raws li lawv tus kheej cov kev xav tau thiab cov nyiaj txiag.Nws yog qhov zoo tshaj plaws los ua qhov kev sim ntsuas gradient dilution amplification txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau nws nws yog qhov zoo tshaj plaws los ua qhov kev sim ua kom nrawm nrawm kom pom qhov ua tau zoo ntawm cov amplification thiab rhiab heev ntawm qhov kub pib Taq enzyme.

Ib qho piv txwv ntawm Foregene'Cov DNA Polymerase:

Foreasy HS Taq DNA Polymerase

Kev piav qhia

Foreasy HS Taq DNA Polymerase yog DNA polymerase qhia nyob rau hauv Escherichia coli engineering kab mob los ntawm gene recombination technology.Cov enzyme tau ua ke nrog cov tshuaj tiv thaiv tshwj xeeb tsis zoo, uas ua rau cov khoom muaj zog tiv taus thiab sib xws, thiab tuaj yeem siv ncaj qha rau cov qauv lysate (Foregene Lysis system) ua tus qauv rau kev kuaj pom.

Daim ntawv thov

Qhov zoo PCR thiab ntau PCR nrhiav pom ntawm cov qauv purified thiab cov qauv tsis huv.

Kev Tswj Xyuas Zoo

1.Tsis muaj exogenous nuclease ua haujlwm

2.PCR txoj kev txhawm rau txheeb xyuas cov DNA seem uas tsis muaj tus tswv

3.Nws tuaj yeem ua kom muaj txiaj ntsig zoo ntawm cov noob ib zaug hauv tib neeg Genome

4.Khoom ntawm chav tsev kub rau ib lub lim tiam, noobvious kev hloov pauv

Cov ntsiab lus ntawm cov khoom: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/