Tsim kev sim PCR SOP los ua tus qauv coj tus cwj pwm ntawm cov neeg ua haujlwm sim.
Tus kws sim ua raws li cov txheej txheem kev ua haujlwm, thiab txo qis PCR cov pa phem uas yuav tshwm sim los ntawm tib neeg los yog tiv thaiv qhov tshwm sim ntawm cov pa phem.Tsis tas li ntawd, tus kws sim yuav tsum muaj kev paub txog kev txawj ntse thiab kev txawj ntse, suav nrog kev paub txog kev ua haujlwm ntawm cov cuab yeej siv, qhia meej txog tag nrho cov txheej txheem ua haujlwm, paub txog cov txheej txheem kev kho mob ntawm cov kab mob sib kis thiab cov txheej txheem kuaj xyuas kom zoo, thiab tuaj yeem txhais cov txiaj ntsig kev xeem kom raug.
Tsim ib lub chaw kuaj PCR tus qauv.
PCR lub chaw soj nstuam tau muab faib ua plaub qhov hauv cov hauv paus ntsiab lus, uas yog thaj chaw npaj reagent, thaj chaw ua piv txwv, thaj chaw amplification, thiab thaj chaw amplification khoom tsom xam.Thawj ob thaj chaw yog thaj chaw ua ntej amplification, thiab ob qhov kawg yog thaj chaw tom qab amplification.Lub pre-amplification cheeb tsam thiab tom qab amplification cheeb tsam yuav tsum tau nruj me ntsis sib cais.Kev sim cov ntaub ntawv, reagents, sau ntawv, cwjmem, tu cov ntaub ntawv, thiab lwm yam, tsuas yog ntws los ntawm lub pre-amplification cheeb tsam mus rau lub post-amplification cheeb tsam, uas yog, los ntawm reagent npaj cheeb tsam → coj mus kuaj cheeb tsam → amplification cheeb tsam → amplification khoom tsom xam cheeb tsam, thiab yuav tsum tsis txhob ntws rov qab.Cov huab cua hauv chav kuaj yuav tsum tau ntws los ntawm thaj chaw ua ntej amplification mus rau thaj tsam tom qab amplification, thiab tsis ntws rov qab.Qhov zoo tshaj plaws PCR kuaj tsim yog qhia hauv qab no:
Daim duab A: Qhov zoo tshaj plaws PCR chav kuaj teeb hom nrog lub siab tsis zoo hauv chav tsis muaj
Daim duab B: Qhov zoo tshaj plaws PCR chav kuaj teeb hom nrog lub siab zoo hauv chav tsis muaj
PCR cov duab teeb tsa hauv daim duab A thiab daim duab B yuav tsum yog hom kev teeb tsa zoo tshaj plaws, thiab chav kuaj nrog cov xwm txheej tuaj yeem xa mus rau hom no rau kev tsim.Rau cov chaw soj nstuam zoo tib yam, nws raug pom zoo tias thaj tsam PCR amplification thiab thaj chaw soj ntsuam cov khoom tuaj yeem raug cais tawm, thiab qhov qhib ntawm lub hau yuav tsum raug txo kom ntau li ntau tau nyob rau hauv cov qauv npaj thiab thaj tsam PCR amplification.Nco ntsoov: Cov khoom lag luam thiab cov khoom siv sim hauv thaj chaw soj ntsuam cov khoom raug txwv nruj kom tsis txhob raug coj mus rau qhov chaw npaj ua qauv thiab thaj chaw PCR amplification.
Yog tias lub chaw kuaj xyuas tsuas yog ua PCR tshawb pom thiab txheeb xyuas, nws raug nquahu kom siv fluorescent quantitative PCR es tsis txhob siv PCR.
Fluorescence quantitative PCR cov txiaj ntsig tau tuaj yeem sau thiab txheeb xyuas los ntawm cov teeb liab fluorescence, yog li tsis tas yuav qhib lub hau rau electrophoresis tom qab cov tshuaj tiv thaiv, uas zam cov khoom PCR paug los ntawm cov khoom xau ntawm cov tshuaj tiv thaiv los tsim cov aerosols.Yog tias koj nce tus naj npawb ntawm lub hau qhib thaum lub sij hawm thauj khoom ntawm gel electrophoresis, aerosol paug yuav tshwm sim.Nws raug pom zoo kom txhawb nqa daim ntawv thov ntawm PCR ntau thiab maj mam hloov cov PCR zoo.
Lub UNG anti-PCR cov khoom kis kab mob yog siv rau cov tshuaj tiv thaiv PCR.
Lub kaw lus siv dUTP hloov dTTP.Tom qab cov tshuaj tiv thaiv PCR, tag nrho cov khoom PCR (DNA fragments) tau koom nrog dUTP;Nyob rau hauv ib puag ncig tom ntej ntawm PCR cov tshuaj tiv thaiv, UNG enzyme ntxiv rau hauv lub cev yog incubated ntawm 37 ° C rau 5 feeb ua ntej PCR, uas muaj peev xwm tshwj xeeb degrade tag nrho DNA fragments uas muaj dUTP, thiab ces ua PCR cov tshuaj tiv thaiv.Qhov no tuaj yeem tshem tawm cov kab mob aerosol los ntawm PCR cov khoom.Cov nyhuv yog pom nyob rau hauv daim duab hauv qab no:
Lus Cim: Rau cov PCR ncaj qha, koj tuaj yeem xaiv cov khoom lag luam ntawm cov khoom tiv thaiv PCR ntawm Foregene.Qhia
Rau cov chaw soj nstuam uas ua qhov loj-loj genotyping kuaj, nws tau pom zoo kom siv UNG anti-PCR cov khoom kis kab mob rau kev sim tshuaj reagents ntxiv rau kev tsim kho cov chaw sim tsim nyog.
Ceeb Toom: Kev siv cov kab ke no tsis tuaj yeem tshem tawm PCR cov khoom sib kis uas twb tau tshwm sim lawm.Yog li ntawd, UNG system yuav tsum tau siv thaum pib ntawm qhov kev ntsuam xyuas cuam tshuam, thiab UNG system yuav tsum tau siv rau PCR amplification, thiaj li tiv thaiv kev kis kab mob ntawm PCR cov khoom cuav.
Nws raug pom zoo kom siv Direct PCR-UNG system ntawm Foregene thaum ua qhov kev sim loj, xws li:
Plant Leaf Direct PCR Kit-UNG;
Plant Seed Direct PCR Kit-UNG;
Tsiaj Tissue Direct PCR Kit-UNG;
Mouse Tail Direct PCR Cov Khoom Siv-UNG;
Zebra Ntses Direct PCR Kit-UNG.
Qhov no series ntawm cov khoom siv los ntawm Foregenetuaj yeem tsis tsuas yog ua PCR nrhiav kom tau sai thiab loj, tab sis kuj tseem tiv thaiv thiab tswj PCR cov khoom paug.
Post lub sij hawm: Mar-19-2021
