Kev yug ntawm PCR
PCR (Polymerase Chain Reaction)
Nws tau ntau tshaj 30 xyoo txij li qhov tsim ntawm polymerase saw cov tshuaj tiv thaiv.Tau ntau tshaj 30 xyoo, tom qab ntau tus kws tshawb fawb thoob plaws ntiaj teb txuas ntxiv ntxiv thiab txhim kho, PCR thev naus laus zis tau dhau los ua qhov dav thiab nquag siv thiab tseem ceeb tshaj plaws txoj kev tshawb fawb tseem ceeb tshaj plaws hauv tag nrho Lub Neej Kev Tshawb Fawb.
Lub TouchDown PCR, Real-Time PCR, Multi PCR, thiab lwm yam tau tsim los ntawm kev siv dav ntawm PCR thev naus laus zis, nrog rau cov tshiab tshiab Digital PCR (digital PCR), tau ua kom muaj txiaj ntsig zoo rau cov kev tshawb fawb ntawm feem ntau ntawm cov kws tshawb fawb tshawb fawb thiab ua kom cov txheej txheem kev loj hlob ntawm niaj hnub Life Sciences, tshwj xeeb tshaj yog cov kev tshawb fawb molecular, tau ua rau tib neeg muaj txiaj ntsig zoo thiab muaj txiaj ntsig zoo.
Qhov tsis xws luag ntawm tsoos PCR technology
complex nucleic acid sib cais thiabrho tawm:
★ Ib txwm siv tshuab PCR: yuav tsum tau
★ PCR muab tau technology: yuav tsum tau
★ DNA thiab RNA cov qauv: qhov sib txawv loj, kev ua haujlwm nyuaj
★ Lub cev muaj kev phom sij: cov tshuaj lom neeg ua phem rau lub cev
Traditional PCR technology thiab derivative technology muaj ib tug prerequisite-nucleic acid sib cais thiab purification
Txhua tus qauv lom neeg yuav tsum tau mus dhau los ntawm cov txheej txheem nyuaj thiab nyuaj ua qauv kom tau txais cov qauv nucleic acid uas ua tau raws li cov kev cai ntawm PCR thev naus laus zis.
Kev sib cais thiab rho tawm ntawm DNA thiab RNA ib txwm yog txoj haujlwm tseem ceeb uas cov kws tshawb fawb txog kev tshawb fawb yuav tsum tau rov ua dua txhua hnub.
Vim muaj qhov sib txawv loj ntawm cov qauv, kev sib cais thiab kev rho tawm ntawm DNA thiab RNA kuj txawv heev.Txoj haujlwm no yuav tsum muaj kev txawj ntse rau cov neeg ua haujlwm siab.Kev sib cais thiab kev rho tawm ib txwm xav tau kev sib cuag mus ntev nrog qee cov tshuaj lom neeg cov tshuaj reagents.Nws yuav ua rau tsis muaj kev puas tsuaj rau tus neeg teb xov tooj lub cev, thiab tseem ua rau muaj kev puas tsuaj ncaj qha thaum lub sijhawm sim.
Nyob rau tib lub sijhawm, rau cov neeg uas muaj ntau cov qauv los kawm, kev sib cais thiab rho tawm cov nucleic acids yog ib txoj haujlwm hnyav heev.
Nucleic acid cais tawm thiab cov khoom siv rho tawm ntawm kev ua lag luam tam sim no paub tab thiab muaj ntau lub npe, tab sis lawv zoo ib yam.Txawm hais tias nws yog silica gel membrane kem centrifugal cov khoom siv los yog cov khoom siv hlau nplaum sib nqus, nws yuav siv sij hawm ntau thiab kim heev.Ntxiv nrog rau cov nqi ntawm cov khoom siv, kuj tseem muaj cov kev cai tshwj xeeb rau cov khoom siv hauv chav kuaj.Lub chaw ua haujlwm automated siv nyob rau hauv txoj kev sib nqus hlaws yog ib qho khoom siv loj heev uas muaj txiaj ntsig zoo, uas yog ib qho kev siv nyiaj loj heev rau chav kuaj.
Hauv cov ntsiab lus
Ua ntej ua qhov kev sim PCR, qhov kev kho ua ntej ntawm cov qauv yog qhov tsis muaj kev zam thiab ib txwm mob taub hau rau cov kws tshawb fawb.Yuav daws qhov teeb meem no li cas thiab seb qhov kev sim PCR tuaj yeem ua tau yam tsis muaj kev sib cais thiab tshem tawm cov nucleic acids ib txwm yog qhov kev xav ntawm feem ntau ntawm cov kws tshawb fawb thiab cov neeg ua haujlwm hauv chaw kuaj mob.
Foregene's Solution
Tom qab xyoo ntawm kev mob siab rau kev tshawb fawb ntawm Direct PCR thev naus laus zis thiab cov khoom siv cuam tshuam, Forgene tau ua tiav los ntawm ntau lub fwj dej thiab ua tiav cov PCR ncaj qha rau ntau hom qauv sib txawv nrog kev muaj zog tiv thaiv thiab yoog raws, tso cai rau cov kws tshawb fawb kom tshem tawm cov kev sib cais thiab txaus ntshai thiab tshem tawm cov nucleic acids.Qhov no yuav txo tau txhua tus neeg txoj kev siv zog, ua kom cov txheej txheem sim ua kom sai, thiab txuag cov nqi tshawb fawb thiab kev sim.
Forgene kev nkag siab thiab kev paub ntawm DirectPCR
Ua ntej, DirectPCR thev naus laus zis yog PCR thev naus laus zis rau ntau yam ntaub so ntswg lom.Raws li cov txheej txheem no, tsis tas yuav cais thiab rho tawm cov nucleic acids, thiab cov qauv ntaub so ntswg ncaj qha siv los ua cov khoom, thiab lub hom phiaj gene primers ntxiv rau PCR cov tshuaj tiv thaiv.
Thib ob, DirectPCR thev naus laus zis tsis yog tsuas yog ib txwm siv DNA template amplification technology, tab sis kuj suav nrog RNA template thim rov qab PCR.
Thib peb, DirectPCR thev naus laus zis tsis yog tsuas yog ua haujlwm ncaj qha rau PCR cov tshuaj tiv thaiv niaj hnub ntawm cov qauv ntaub so ntswg, tab sis kuj suav nrog Real-Time qPCR cov tshuaj tiv thaiv, uas yuav tsum muaj cov tshuaj tiv thaiv kom muaj peev xwm tiv thaiv keeb kwm yav dhau los cuam tshuam fluorescence thiab antagonize endogenous fluorescence quenchers.
Plaub, cov qauv ntaub so ntswg tsom los ntawm DirectPCR thev naus laus zis tsuas yog yuav tsum tau tso tawm cov qauv nucleic acid thiab tsis tshem cov proteins, polysaccharides, ntsev ions, thiab lwm yam uas cuam tshuam rau PCR cov tshuaj tiv thaiv.Qhov no yuav tsum muaj cov nucleic acid polymerase thiab PCR Mix hauv cov tshuaj tiv thaiv kom muaj kev tiv thaiv kev thim rov qab thiab hloov tau zoo, thiab tuaj yeem ua kom muaj kev ua haujlwm enzyme thiab rov ua dua qhov tseeb hauv cov xwm txheej nyuaj.
Thib tsib, cov qauv ntawm cov ntaub so ntswg tsom los ntawm DirectPCR thev naus laus zis tsis tau raug kho rau nucleic acid enrichment, thiab cov qauv tsim muaj tsawg heev, uas yuav tsum tau muaj qhov ua tau zoo heev thiab ua kom muaj zog ntawm cov tshuaj tiv thaiv.
Xaus
DirectPCR thev naus laus zis yog ib qho tseem ceeb tshaj plaws kev tsim kho thiab kev tsim kho tshiab hauv 30 xyoo dhau los txij li yug los ntawm PCR thev naus laus zis.Forgene muaj thiab yuav txuas ntxiv ua tus pioneer thiab innovator ntawm cov thev naus laus zis no.
Daim ntawv thov kev cia siab ntawm DirectPCR thev naus laus zis yog qhov dav heev.Kev txhim kho tas mus li thiab kev txhawb nqa ntawm cov thev naus laus zis no yuav ua rau muaj kev hloov pauv tsis zoo rau kev tshawb fawb thiab kev tshuaj xyuas ua haujlwm.Nov yog PCR thev naus laus zis hloov pauv.
Post lub sij hawm: Feb-21-2017
