Qhov zoo tshaj plaws ntawm cov tshuaj tiv thaiv PCR yog nws lub peev xwm loj loj thiab muaj kev nkag siab zoo heev.Txhawm rau txhim kho PCR kev ua tau zoo thiab kev tshawb pom kev ua tau zoo, peb tau cog lus los txhim kho PCR amplification muaj peev xwm thiab nrhiav kom tau rhiab heev, tab sis mob taub hau yog nyob rau hauv cov txheej txheem ntawm kev sim.Qhov tsis zoo tshwm sim feem ntau tshwm sim, thiab tsawg heev ntawm cov qauv sib kis los yog PCR cov khoom sib kis yuav ua rau muaj qhov tsis zoo hauv qhov kev sim.
Tsib hom PCR khoom paug
Muaj ntau yam laj thawj rau PCR paug, uas tuaj yeem muab faib ua pawg hauv qab no:
Cov kab mob kis kab mob feem ntau yog tshwm sim los ntawm kev sib kis ntawm lub thawv rau kev sau cov qauv, lossis thaum cov khoom xa tuaj, nws tau xau tawm ntawm lub thawv vim xoob sealing, los yog cov khoom xa mus rau sab nraud ntawm lub thawv, uas ua rau muaj kev sib kis;Kev kis kab mob ua rau muaj kab mob sib kis ntawm cov qauv;Qee cov kab mob microbial, tshwj xeeb tshaj yog cov kab mob, tuaj yeem kis tau nrog aerosols lossis tsim aerosols, ua rau muaj kev sib kis.
Lub ntsiab yog vim li cas thaum lub sij hawm npaj ntawm PCR reagents, cov qauv phom, thawv, ob chav distilled dej thiab lwm yam kev daws teeb meem yog paug los ntawm PCR nucleic acid template.
Hauv cov chaw kuaj mob molecular biology thiab qee lub chaw kuaj mob uas siv cov cloned plasmids ua cov kev tswj zoo, qhov teeb meem ntawm cloned plasmid paug kuj tseem muaj.Vim hais tias cov ntsiab lus ntawm cloning plasmid nyob rau hauv chav tsev ntim yog siab heev, thiab ntau cov cuab yeej thiab reagents yog xav tau nyob rau hauv lub purification txheej txheem, thiab plasmid nyob rau hauv cov cell muaj feem ntau yuav kis tau tus kab mob vim muaj zog loj hlob thiab muaj peev xwm ntawm cov nyob.
Kev kis kab mob ntawm cov khoom lag luam amplified yog qhov teeb meem ntau tshaj plaws hauv cov tshuaj PCR.Vim tias cov ntawv luam ntawm PCR cov khoom lag luam loj (feem ntau 1013 daim ntawv luam / ml), uas yog siab dua li qhov txwv ntawm PCR nrhiav pom tus lej, qhov tsawg heev ntawm PCR cov khoom paug tuaj yeem ua rau muaj qhov tsis zoo.
Aerosol muaj kuab paug yog feem ntau yuav kis tau tus kab mob ntawm PCR cov khoom, thiab nws kuj yog ib qho yooj yim yuav tau saib xyuas.Nws yog tsim los ntawm kev sib txhuam ntawm cov kua dej thiab cov huab cua.Feem ntau, cov kab mob aerosol tuaj yeem tsim tau thaum lub hau qhib, thaum tus qauv yog aspirated, lossis txawm tias thaum lub raj tshuaj tiv thaiv yog shaken.Raws li kev suav, aerosol particle tuaj yeem muaj 48,000 daim ntawv luam, yog li cov pa phem los ntawm nws yog ib qho teeb meem uas tsim nyog tau txais kev saib xyuas tshwj xeeb.
Tshwj xeeb, cov chaw kuaj sim feem ntau siv tib khub ntawm primers los kuaj cov noob caj noob ces.Nyob rau tib lub sijhawm, PCR cov khoom sib kis ntau yuav tshwm sim hauv qhov chaw kuaj.Thaum muaj cov kab mob sib kis no tshwm sim, nws nyuaj rau kev tshem tawm hauv lub sijhawm luv luv.
Rau thawj peb hom kev ua qias tuaj, peb tuaj yeem siv txoj kev zoo kom tsis txhob muaj, tab sis cov pa phem los ntawm PCR cov khoom yog qhov nyuaj rau kev tiv thaiv, tshwj xeeb tshaj yog nyob rau hauv kev tsim kho ntawm PCR lub chaw soj nstuam uas tsis yog tus qauv.Nyob rau hauv cov txheej txheem PCR, thaum lub pipette taub hau nqus thiab tshuab cov kua, thiab lub PCR raj cover yog qhib, ib tug aerosol yuav tsim.Cov DNA molecules nqa los ntawm aerosol (ib qho aerosol tuaj yeem nqa tau kaum txhiab DNA) nyuaj rau kev tshem tawm vim tias lawv ntab saum huab cua.Thaum qhov kev sim tom ntej ntawm PCR raug qhia, qhov tsis zoo yuav tshwm sim tsis muaj tseeb.
Raws li pom nyob rau hauv daim duab hauv qab no, qhov kev tswj tsis zoo kuj tseem ua kom muaj txiaj ntsig zoo sib xws:
Thawj feem ntawm qhov teeb meem no ntawm PCR kuab paug thiab kev tiv thaiv yog qhia ntawm no.Qhov teeb meem tom ntej no yuav coj koj mus rau ntu thib ob "Kev Tiv Thaiv PCR cov khoom sib kis", yog li nyob twj ywm!
Post lub sij hawm: Jul-25-2017
