Cov nram qab no tsom xam cov teeb meem tshwm sim nyob rau hauvBuccalswab/FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

Lub purification kem yog clogged

Nyob rau hauv cov khoom no, nyob rau hauv lub genomic DNA extraction ua hauj lwm, lub purification kem yog ncaj qha adsorbed ntawm cov qauv enzymatic lysis sib tov yam tsis muaj centrifugation kauj ruam, thiab purification kem yuav raug thaiv vim tsis tiav enzymization thiab siab viscosity ntawm cov qauv.

Cov ua tau hauv qab no yog raws li nram no:

1. Tsis tiav enzymatic digestion ntawm cov ntaub so ntswg kuaj.

Kev pom zoo: Lub sijhawm ua piv txwv ntawm Foregene Protease tuaj yeem txuas ntxiv kom tsim nyog lossis tus supernatant tuaj yeem raug coj mus tom qab centrifugation ntawm 12,000 rpm (~ 13,400× g) rau 5 feeb.

2. Kev siv cov ntaub so ntswg ntau dhau los yog cov ntaub so ntswg loj.

Kev pom zoo: Nws yog qhov zoo tshaj kom tsis pub tshaj 1Buccal swab hauv tus qauv;Yog tias tus qauv loj dhau, nce qhov ntau npaum ntawm Buffer ST1, Foregene Protease, tsis ST2 raws li.

3. Cov qauv viscosity siab dhau lawm.

Kev pom zoo: Cov qauv tuaj yeem tsim nyog diluted nrog 10 mM ntawm Tris-HCl ua ntej genomic DNA rho tawm.

4. Cov seem ntawm daim npav ntshav tau nqus.

Kev pom zoo: Lub sijhawm centrifugation ib ntus ntawm kauj ruam 6 ntawm ntshav chaw (FTA Card) genomic extraction tuaj yeem txuas ntxiv kom tsim nyog.

Tsawg tawm los lossis tsis muaj DNA

Muaj ntau ntau yam uas cuam tshuam rau genomic DNA yield, nrog rau cov qauv hauv paus chiv keeb, cov qauv khaws cia, qauv npaj, kev tswj xyuas, thiab lwm yam.

Genomic DNA tsis tuaj yeem tau txais thaum lub sijhawm rho tawm

Cov ua tau yog raws li nram no:

1. Kev khaws cia tsis raug ntawm cov qauv lossis khaws cia ntev dhau los ua rau genomic DNA degradation.

Cov lus pom zoo: Lub qhov ncauj swabs yuav tsum zoo dua yog cov qauv tshiab, thiab nws tsis pom zoo kom siv cov swabs khaws cia rau kev ua haujlwm ntawm DNA genomic extraction;Cov ntshav kuaj ntshav yuav tsum xyuas kom meej tias qhov zoo yog tsim nyog thiab lub sijhawm cia yuav tsum tsis txhob ntev dhau.

2. Kev siv cov ntaub so ntswg tsawg dhau yuav ua rau tsis muaj kev rho tawm ntawm cov DNA sib txuas.

Kev pom zoo: Ua raws li covbucca ual swab sampling cov lus qhia hauv phau ntawv qhia ua haujlwm, thiab so ntau zaus li sai tau kom cov hlwb txaus tuaj yeem txuas rau lub qhov ncauj swab rau genomic DNA extraction;rau cov ntshav qhov chaw coj mus kuaj, qhov chaw txiav cov ntshav tuaj yeem tsim nyog ntxiv.

3. Foregene Protease yog khaws cia tsis raug, ua rau txo qis hauv nws txoj haujlwm lossis kev ua haujlwm tsis zoo.

Kev pom zoo: Paub meej tias qhov chaw cia khoom ntawmlub Foregene Protease lossis hloov nws nrog Foregene Protease tshiab rau cov tshuaj tiv thaiv enzymatic.

4. Kev khaws cia tsis raug ntawm cov khoom siv lossis lub sijhawm khaws cia ntev dhau lawm, ua rau qee yam khoom siv hauv cov khoom siv tsis ua haujlwm.

Kev Pom Zoo: Yuav khoom tshiabBuccal DNA swbmuab cais nyob ib leeg cov khoom siv rau cov txheej txheem cuam tshuam.

5. Buffer WB tsis ntxiv ethanol kiag li.

Kev pom zoo: Paub meej tias tsis WB ntxiv qhov tseeb ntim ntawm ethanol.

6. Lub eluent tsis raug ntxiv rau cov yeeb yaj kiab silicone.

Kev pom zoo: Ntxiv 65°Cpre-warmed eluent tee mus rau nruab nrab ntawm silicone membrane thiab tawm hauv chav sov li 5 min kom nce elution efficiency.

Lo-yield genomic DNA cais

Cov ua tau hauv qab no yog raws li nram no:

1. Kev khaws cia tsis raug ntawm cov qauv lossis khaws cia ntev dhau los ua rau genomic DNA degradation.

Daim ntawv pom zoo: Cov qhov ncauj qhov ncauj yog qhov zoo dua tshiab coj mus kuaj, thiab khaws cia swabs yuav tsum tsis txhob siv rau kev rho tawm DNA genomic.

2. Yog tias cov qauv ntawm cov ntaub so ntswg me me dhau lawm, cov ntsiab lus ntawm DNA genomic extracted yuav tsawg dua.

Kev pom zoo: Ua raws li cov lus qhia ntawm qhov ncauj swab sampling nyob rau hauv phau ntawv qhia kev khiav hauj lwm, so kom ntau li ntau zaus kom cov hlwb txaus tuaj yeem txuas rau lub qhov ncauj swab rau genomic DNA extraction.

3. Foregene Protease yog khaws cia tsis raug, ua rau txo qis hauv nws txoj haujlwm lossis kev ua haujlwm tsis zoo.

Kev pom zoo: Paub meej tias qhov chaw cia khoom ntawmthe Foregene Protease los yog hloov nws nrog ib qho tshiab Foregene Protease rau cov tshuaj tiv thaiv enzymatic.

4. Muaj teeb meem.

Kev pom zoo: Siv Buffer EB rau elution;yog siv ddH₂O lossis lwm yam eluents, paub meej tias pH ntawm eluate nyob nruab nrab ntawm 7.0-8.5.

5. Lub eluate tsis raug ntxiv dropwise.

Kev pom zoo: Ntxiv eluent tee rau hauv nruab nrab ntawm silicone membrane thiab tawm hauv chav sov li 5 min kom nce elution efficiency.

6. Cov kua elution accumulates tsawg dhau.

Kev pom zoo: Siv eluent rau genomic DNA elution raws li xav tau hauv cov lus qhia, tsawg kawgtsis tsawg tshaj 15μl.

Lua purity of genomic DNAcais

Tsawg genomic DNA purity tuaj yeem ua rau tsis ua haujlwm lossis tsis txaus siab ntawm cov kev sim hauv qab, xws li: enzymes tsis tuaj yeem txiav qhib, PCR tsis tuaj yeem tau txais cov noob ntawm kev txaus siab, thiab lwm yam.

Cov ua tau yog raws li nram no:

1. Heteroprotein pollution, RNA kuab paug.

Kev tsom xam: Cov kab purification tsis tau ntxuav siv Buffer PW;Buffer PW ntxuav purification kem tsis tau ntxuav siv qhov tseeb centrifugal ceev.

Kev pom zoo: xyuas kom meej tias tsis muaj nag lossis daus hauv lub supernatant ua ntej ntxiv ethanol;nco ntsoov ntxuav lub purification kem raws li cov lus qhia, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.

2. impurity ion muaj kuab paug.

Kev tshuaj ntsuam xyuas: Buffer WB ntxuav purification kem raug tshem tawm lossis ntxuav tsuas yog ib zaug, ua rau cov khoom seem ionic.

Kev pom zoo: Nco ntsoov ntxuav Buffer WB 2 zaug raws li qhia kom tshem tawm cov ions seem ntau li ntau tau.

3. RNA enzyme paug.

Kev tsom xam: Cov RNases txawv teb chaws tau ntxiv rau qhov tsis muaj;Buffer PW ntxuav kev ua haujlwm tsis raug, ua rau RNase residues, cuam tshuam rau hauv qab RNA kev sim ua haujlwm, xws li hauv vitro transcription.

Kev pom zoo: Foregene series nucleic acidisolaCov khoom siv tion tuaj yeem tshem tawm RNA yam tsis muaj RNase ntxiv,li no buccal Swab/FTA Card DNA Isolation Kitxav tau't ntxiv RNase;Nco ntsoov ua raws li cov lus qhia rau Buffer PW ntxuav purification kem, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.

4. Ethanol residue.

Kev tshuaj xyuas: Buffer WB tsis ua qhov khoob raj centrifugation tom qab ntxuav lub purification kem.

Kev pom zoo: Ua qhov raug khoob raj centrifugation ua haujlwm raws li cov lus qhia.

5. Lwm yam impurity muaj kuab paug.

Kev Ntsuam Xyuas: Cov qauv khaws tseg lossis cov qauv tshwj xeeb tsis raug ua ntej.

Kev pom zoo: Ua tib zoo pretreat tus qauv raws li qhia.