Nucleic acid extraction yog ib qho txuj ci qhia txog kev sim molecular biology.Txawm hais tias nws tuaj yeem ua tus tswv thiab siv cov txuj ci no kom tau txais txiaj ntsig kuj tau dhau los ua qhov yuav tsum tau ua ua ntej rau cov neeg sim tom qab txhawm rau hloov mus rau kev sim masters.
Raws li cov ntaub ntawv tshawb fawb sib txawv, cov kws sim tau muab faib ua peb hom hauv qab no:
Tsiaj loj, cog loj, Microbiology loj
Txhua hom kev sim yuav txuas ntxiv luam cov ntawv luam los tsim cov txheej txheem tshwj xeeb rau cov qauv tshwj xeeb.
Niaj hnub no, tus editor xav nqa qee yam khoom siv thiab khoom siv uas tsis muaj nyob hauv qhov taub rau cov neeg sim.Nrog lawv, koj tuaj yeem hloov kho sai sai hauv txhua qhov kev qhia.Nws tsis yog npau suav los ua ib tug Vajtswv sim.
Qhov teeb meem no yog rau cov kws ua haujlwm cog qoob loo (cov kws sim ntawm lwm cov haujlwm tseem tuaj yeem kawm los ntawm nws ~).
Kuv ntseeg hais tias txhua tiam ntawm cov neeg sim yuav tau hnov txog cov classic cog nucleic acid extraction txoj kev dhau los ntawm ib tiam dhau ib tiam los ntawm lawv cov thawj coj.Peb lub npe loj hauv cov nroj tsuag extraction kev lag luam.
Txawm hais tias cov txiv neej loj yog cov zoo heev, lawv tseem muaj qee qhov kev tsis txaus ntseeg.
Txoj kev TRizol
Feem ntau siv los rho tawm
RNA
● Toxic thiab cumbersome
● Muaj cov kab mob genome
CTAB method
Feem ntau siv los rho tawm
genomeDNA
● Lub sijhawm siv
● Ntau qhov nyuaj rau tshem tawm CTAB los ntawm kev ntxuav dua ntsev, ib qho me me ntawm cov khoom seem yuav muaj kev cuam tshuam loj rau kev ua haujlwm enzyme
SDS method
Feem ntau siv los rho tawm
plasmid
● Tsis haum rau cov nroj tsuag uas muaj cov ntsiab lus polysaccharide siab
● Protein paug
Nyob rau hauv lub neej niaj hnub no nrog ob-ceev hloov tshiab iterations, ib txwm txoj kev ntawm kev ua hauj lwm tsis muaj peev xwm ua tau raws li cov kev xav tau ntawm cov neeg sim, thiab cov cuab yeej hloov khoom dua tshiab yuav tshwm sim.
Foregene Nucleic Acid Extraction Kit tau raug sim N zaug los ntawm ntau tus neeg sim.Nws yog ib qho yooj yim rau kev khiav lag luam, tsis siv cov tshuaj lom reagents, thiab tsis ntxiv cov enzymes.Cov nucleic acid tau txais yog cov purity siab, uas yog qhov tseem ceeb rau kev ua haujlwm txhua hnub ntawm cov neeg sim ua haujlwm zoo.
Foregene RNA Isolation Kit
Ntawm no, kuv tseem yuav nthuav tawm cov txheej txheem zais cia rau cov neeg sim, uas yog xaiv txoj kev rho tawm tsim nyog raws li hom nroj tsuag (txoj kev los txiav txim siab hom nroj tsuag yog qhov kawg ntawm tsab xov xwm!), vim tias qee cov nroj tsuag polysaccharides thiab polyphenols muaj cov ntsiab lus siab, thiab cov txheej txheem ib txwm ua rau tuag taus.Tsis txaus los tswj.
Zoo, nws zoo li no ...
Txoj kev TRIzol ncaj qha txhos caug hauv cov qauv nrog cov qib siab ntawm polysaccharides thiab polyphenols xws li txiv tsawb nplooj, nplooj ginkgo thiab paj rwb nplooj.
Rau lub hom phiaj no, Foregene tau tsim ob lub cuab yeej zoo rau cov neeg sim.Cov nroj tsuag RNA rho tawm cov khoom siv uas tsis tshua muaj polysaccharide thiab polyphenol cov ntsiab lus thiab siab polysaccharide thiab polyphenol cov ntsiab lus tuaj yeem tua cov yeeb ncuab hauv lub hom phiaj, kom cov neeg sim tsis ntshai ntawm cov nroj tsuag sib txawv.Tsuas yog hloov cov khoom siv ncaj qha!
Ntxiv nrog rau qhov yuav tsum tau hloov kho txoj kev rho tawm, cov qauv ua ntej ua haujlwm kuj yuav tsum tau hloov kho dua tshiab.
Vim yog cov qauv tshwj xeeb ntawm cov qauv cog, cov hauv paus hniav thiab cov stems tougher ntawm qee cov nroj tsuag yog qhov nyuaj rau zom thiab zom sib npaug.
Hauv kev lag luam sib tsoo, kwv yees li 80% ntawm cov neeg siv cov kua nitrogen rau kev sib tsoo, thaum tsawg dua 20% siv hluav taws xob sib tsoo thiab sib tsoo hlaws oscillation.
Txawm hais tias cov kua nitrogen sib tsoo yog pheej yig thiab siv tau yooj yim, nws yog npau suav phem rau cov neeg sim nrog cov qauv loj thov kom zom txhua zaus thiab siv zog!Yog tias lub mortar tsis raug ntxuav, nws yuav ua rau muaj kev sib kis ntawm cov qauv.
Cov txiaj ntsig ntawm kev sib tsoo hluav taws xob sib txawv ntawm ib tus neeg mus rau ib tus neeg, thiab kev ua haujlwm ntawm cov neeg sim yuav muaj kev cuam tshuam ntau dua rau kev ncaj ncees ntawm nucleic acid, thiab kev ua haujlwm kub qis yog tsim nyog.
Yog li, kev sib tsoo hlaws oscillation tuaj yeem suav tias yog cov cuab yeej sib tsoo tam sim no yooj yim thiab siv tau zoo.
Cov hom oscillation muaj xws li ntsug oscillation, kab rov tav reciprocating oscillation thiab peb-dimensional (kab rov tav oscillation).Txawm li cas los xij, Foregene cov ntaub so ntswg crusher tawg los ntawm cov qauv uas twb muaj lawm thiab siv txoj kev 4D oscillation tshiab los ua kom sib tsoo ntau dua.
Ua tau raws li ntau yam qauv xav tau, yooj yim rau kev khiav lag luam
Cim 4D crushing tshuab, lub crushing efficiency yog nce los ntawm 20%
Cov qauv yog kaw tag nrho, tsis muaj kab mob sib kis
Nyob ntsiag to, ≤56 decibels
Sib piv cov txiaj ntsig ntawm kev sim mus rau nthwv dej ~
Cov qauv kuaj: 50mg nplej nplooj (cov ntsiab lus tsawg ntawm polysaccharides thiab polyphenols)
Extract nucleic acid hom: RNA
Qhov hnyav: 5μl
Cov khoom siv rho tawm siv yog Foregene Plant Total RNA Extraction Kit (RE-05011)
Sib piv cov ntaub ntawv thiab cov duab electrophoresis, nws tuaj yeem pom tias qhov concentration thiab purity ntawm RNA rho tawm tom qab lub tshuab sib tsoo yog siab dua li cov kua nitrogen sib tsoo.Nyob rau tib lub sijhawm, vim tias lub tshuab sib tsoo tuaj yeem rhuav tshem cov qauv ntau dua, RNA me me tuaj yeem muab rho tawm zoo dua thiab kev ncaj ncees ntawm RNA tuaj yeem tswj tau.
Cov qauv kuaj: 50mg ginkgo nplooj (siab polysaccharide thiab polyphenol cov ntsiab lus)
Extract nucleic acid hom: RNA
Qhov hnyav: 5μl
Siv Foregene Polysaccharide Polyphenol Cog Tag Nrho RNA Extraction Kit (RE-05021)
Ua tibzoo saib cov qauv no nrog cov ntsiab lus siab ntawm polysaccharides thiab polyphenols.Cov nyhuv tseem pom tseeb dua.Nrog tib lub purity, qhov concentration ntawm lub tshuab sib tsoo RNA yog ze li ntawm 5 npaug ntawm cov kua nitrogen sib tsoo, thiab RNA me me tseem pom faintly ~
Yog tias koj xav tias tsis muaj ib qho ntawm cov cuab yeej saum toj no tau nrawm txaus, ces tus editor yuav tsum tuaj nrog cov khoom siv kawg!
Cog Direct PCR series
Lub cim tshwj xeeb ntawm cov cuab yeej no yog tias cov nroj tsuag nplooj lossis cov noob tsis tas yuav tsum tau hauv av thiab tawg, thiab ncaj qha muab pov rau hauv EP raj rau lysis, thiab cov lysis sib tov tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.Nws yog yooj yim li ib kauj ruam sai!Nyob rau tib lub sijhawm, ncaj qha PCR series ntawm Foregene Plants yog nruab nrog UNG tiv thaiv kev ua qias tuaj, yog li cov neeg sim tsis txhawj xeeb txog cov pa phem hauv chav kuaj thiab tuaj yeem ua qhov kev sim tau yooj yim.
Cov lus qhia txiav txim:
Yuav txiav txim seb nws puas yog polysaccharide polyphenol cog
Noj li 20mg cov ntaub so ntswg thiab muab tso rau hauv ib lub raj 1.5ml centrifuge huv, ntxiv 200ul 200mM NaOH tov, muab tso rau ntawm 95 ℃ rau 10min, saib cov xim hloov tom qab lysis
1. Yog tias cov xim ntawm lysate yog ntsuab los yog lub teeb daj, nws txhais tau hais tias cov ntsiab lus ntawm cov ntaub so ntswg polyphenols tsawg, thiab nws raug nquahu kom siv Foregene Plant series.
2. Yog tias cov lysate yog xim av-daj lossis xim av-liab, nws txhais tau hais tias cov ntaub so ntswg polyphenol cov ntsiab lus siab, thiab nws raug pom zoo kom siv Foregene Polysaccharide Polyphenol Plant Plus Series
Post lub sij hawm: Aug-20-2021
