Muaj ob hom kev pheej hmoo tseem ceeb hauv PCR lub chaw soj nstuam: kev pheej hmoo biosafety thiab nucleic acid paug txaus ntshai.Cov qub ua phem rau tib neeg thiab ib puag ncig, thiab qhov kawg cuam tshuam rau cov txiaj ntsig ntawm kev xeem PCR.Kab lus no yog hais txog PCR lub chaw soj ntsuam kev pheej hmoo soj ntsuam cov ntsiab lus thiab cov theem kev pheej hmoo coj los rau koj.

01 Division of the PCR Laboratory

1. Molecular biology kuaj kuaj

Raws li qhov yuav tsum tau muaj ntawm Tshooj 1.1 ntawm Cov Txheej Txheem Txheej Txheem Txheej Txheem rau Kev Tshuaj Ntsuam Xyuas Gene Amplification Testing Laboratories, PCR lub chaw soj nstuam feem ntau muaj plaub qhov chaw: reagent cia thiab npaj cheeb tsam, thaj chaw npaj cov khoom, thaj chaw amplification, thiab thaj chaw amplification khoom tsom xam.Yog tias siv cov txheej txheem fluorescent PCR tiag tiag, thaj chaw nthuav dav thiab thaj chaw tsom xam tuaj yeem ua ke rau hauv ib cheeb tsam;yog siv tag nrho automated PCR analyzer, qhov chaw npaj cov qauv, thaj chaw amplification thiab thaj chaw tsom xam tuaj yeem ua ke rau hauv ib cheeb tsam.

"Phau Ntawv Qhia Txog Tus Kab Mob Coronavirus Tshiab Nucleic Acid Test nyob rau hauv Cov Tsev Kho Mob (Tshwj Xeeb Version 2)" qhia tias nyob rau hauv txoj cai, cov chaw soj nstuam ua cov tshuaj tiv thaiv kab mob coronavirus tshiab yuav tsum teeb tsa thaj chaw hauv qab no: reagent cia thiab npaj thaj chaw, thaj chaw npaj cov qauv, nthuav dav thiab thaj chaw tshuaj xyuas khoom.Peb qhov chaw no yuav tsum muaj kev ywj pheej ntawm ib leeg hauv lub cev, thiab tsis tuaj yeem sib txuas lus ncaj qha nrog huab cua.

2. Chav ua qauv npaj

Txawm hais tias cov qauv tuaj yeem npaj tau yooj yim hauv thaj chaw npaj cov qauv, chav ua qauv tshwj xeeb tseem yuav tsum tau ua thaum cuam tshuam nrog cov qauv nyuaj thiab ntau cov qauv.Chav ua qauv npaj muaj kev pheej hmoo siab ntawm kev nyab xeeb lom neeg thiab nucleic acid paug.

3. Chav kho khib nyiab

Kev kho cov khib nyiab tsis raug yuav ua rau muaj kev pheej hmoo loj ntawm biosafety thiab nucleic acid paug rau hauv chav kuaj.Yog li ntawd, chav kho khib nyiab yuav tsum tau saib xyuas tsis tu ncua.

02 Cov ntsiab lus saib xyuas kev pheej hmoo hauv PCR lub chaw soj nstuam

Cov chav kuaj sib cais tau muab faib rau hauv chav ua qauv npaj, reagent cia thiab chaw npaj, thaj chaw npaj cov qauv, thaj chaw nthuav dav thiab cov khoom lag luam tsom xam, thiab chav kho khib nyiab.

Raws li hom qauv ntawm qhov chaw, nws muab faib ua qhov chaw, ntsuas, qauv, kev saib xyuas ib puag ncig thiab pipette.

Qhov kev pheej hmoo theem ntawm qis mus rau siab los ntawm ib lub hnub qub★ mus rau peb lub hnub qub★★★.

1. Sample npaj chav:

Nws yog siv rau kev sau npe, kev npaj thiab inactivation ntawm cov qauv, thiab qhov kev pheej hmoo ntawm kev nyab xeeb yog qhov siab tshaj plaws.Vim tias cov qauv tsis tau muab rho tawm thiab nthuav dav, tshwj tsis yog cov pipettes uas nquag tuaj rau hauv kev sib cuag nrog cov qauv, qhov kev pheej hmoo ntawm cov kab mob nucleic acid hauv lwm qhov chaw yog tsawg.

1-4 Sampling ntawm cov ntsiab lus saib xyuas

5-8 Sampling ntawm qhov chaw saib xyuas

9-12 soj ntsuam point sampling

1. Reagent cia thiab npaj cheeb tsam:

Nws yog siv rau kev npaj cov reagents, dispensing ntawm reagents thiab kev npaj ntawm amplification cov tshuaj tiv thaiv sib tov, nrog rau kev cia thiab npaj cov consumables xws li centrifuge raj thiab pipette tswv yim.Tsis muaj kev sib cuag ncaj qha nrog cov qauv thiab tsis muaj qhov zoo nucleic acid nyob rau hauv cheeb tsam no, yog li qhov kev pheej hmoo ntawm biosafety thiab nucleic acid paug yuav tsawg.

13-16 Sampling ntawm cov ntsiab lus saib xyuas

17-22 Sampling ntawm kev saib xyuas
3. Kev npaj cov ntsiab lus

Nws yog siv los qhib lub thawv hloov pauv, tsis ua haujlwm ntawm cov qauv (thaum tsim nyog), extract nucleic acid thiab ntxiv rau hauv amplification cov tshuaj tiv thaiv raj, thiab lwm yam. Qhov chaw no yuav koom nrog kev ua thiab qhib cov qauv, qhov kev pheej hmoo ntawm kev nyab xeeb yog siab, thiab nucleic acid extraction yog nqa tawm, thiab kev pheej hmoo ntawm nucleic acid paug yog nruab nrab mus rau siab.

29 Sampling ntawm cov ntsiab lus saib xyuas

4. Amplification thiab khoom tsom xam cheeb tsam:

Siv rau nucleic acid amplification.Thaj chaw no tsis koom nrog kev ua qauv, thiab kev pheej hmoo ntawm kev nyab xeeb lom neeg tsawg.Lub nucleic acid amplification feem ntau yog nyob rau hauv cheeb tsam no, thiab kev pheej hmoo ntawm nucleic acid paug yog siab tshaj.

38 Sampling ntawm cov ntsiab lus saib xyuas
5. Chav kho khib nyiab:

Siv rau kev ua haujlwm siab ntawm cov qauv.Cov kev pheej hmoo ntawm kev nyab xeeb lom neeg muaj feem cuam tshuam nrog kev ua cov qauv hauv cheeb tsam no yog qhov siab.Cov khoom lag luam nucleic acid amplification tau pom zoo kom kho raws li kev kho mob pov tseg.Kev kub siab tsis pom zoo, thiab kev pheej hmoo ntawm nucleic acid paug yog tsawg.

43-44 Sampling ntawm cov ntsiab lus saib xyuas

03 Ua

Lub sijhawm no peb teev 44 cov ntsiab lus saib xyuas.Nws kwv yees tias muaj coob tus neeg yuav tsum nug, lawv puas yuav tsum tau ua ntau cov ntsiab lus?Yog, ua txhua yam!Kuv xav kom koj ua ntej ua qhov kev ntsuam xyuas kev pheej hmoo ntawm koj tus kheej lub chaw kuaj mob, uas tuaj yeem ua tau raws li qhov kev pheej hmoo ntawm qhov siab mus rau qis, koj tuaj yeem saib xyuas tib hom qauv ua ke, lossis koj tuaj yeem tsim cov qauv qauv rau kev saib xyuas tsis tu ncua.Nyob rau hauv luv luv, txhua lub chaw soj nstuam tuaj yeem ua nws txoj kev npaj ua raws li nws qhov xwm txheej.Qhov kev pheej hmoo loj tshaj plaws rau cov chaw kuaj sim yog tsis quav ntsej txog qhov txaus ntshai.