PCR Heroᵀᴹ (Nrog Dye)

PCR Heroᵀᴹ (Nrog Dye)

Cov Khoom Siv Qhia:

Qhov 2X Tiag PCR Yooj YimTM  Mix-SYBR muab los ntawm Lub Sijhawm PCR Yooj Yooj YimTM-SYBR ntsuab Kuv cov khoom siv tshiab yog qhov khoom siv tshiab premix uas siv SYBR Green I rau Lub Sijhawm PCR ua kom nrov dua, uas tuaj yeem txhim kho tau cov khoom lag luam tshwj xeeb thiab cov tshuaj tiv thaiv. ROX muab raws li kev tswj xim sab hauv. Lub fluorescence siv ntawm cov khoom siv no yog 3-5 zaug ntawm cov khoom uas zoo sib xws, uas tuaj yeem xav paub ntau dua thiab piav qhia meej txog qhov kev nkag siab ntawm lub hom phiaj qauv DNA.

2X Tiag PCR Yooj YimTM  Mix-SYBR muaj Foregene lub cim kub-pib Taq DNA Polymerase. Piv nrog rau kev ua kom zoo li lub cev Taq, nws muaj qhov zoo ntawm kev ua kom nrov loj, muaj zog muaj peev xwm nthuav dav thiab tsis tshua muaj qhov tsis tseem ceeb. Nws tuaj yeem txo qhov tsis meej tshwj xeeb thiab txhim kho qhov tseeb ntawm PCR.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

5ml, 10ml, 40ml

Cov khoom siv Cheebtsam

0.1U Taq Hero /μl

400μM dNTPs

20mM Tris-HCl (pH8.8)

100mM KCl

5mM MgCl2

Lwm yam stabilizers thiab ua kom zoo dua
DNA Chaw Tos Tsheb

Nta & qhov zoo

■  Siab ncaj ncees: 6 lub sijhawm uas ntawm Taq enzyme zoo tib yam;

Ampl Ua kom nrov loj dua: kev ua kom nrov zog yog 5-10sec / kb, uas yog 6-12 zaug ntawm qhov tseeb ntawm Taq enzyme;

Ntau cov qauv coj ua tau yooj yim: Nws tuaj yeem muaj peev xwm nthuav dav ntau yam DNA nrhav qauv nrog siab GC cov ntsiab lus;

■ Ntau qhov kev ua kom nrov zog: qis dua kev ua kom nrov dua li cov Taq enzyme;

■ Kev tiv thaiv ib puag ncig muaj zog dua: muab tso rau ntawm 37°C rau ib lim piam, khaws cia ntau dua 90% kev ua;

Has Nws muaj 5 ' 3 ' Kev ua haujlwm DNA polymerase thiab 5 '3 ' kev ua tsaug, tsis muaj 3 ' 5 ' exonuc thov kev ua si.

Cov khoom siv tau npaj ua ntej nrog Electrophoresis Chaw thau khoom tsis tiav, tom qab PCR tiav, cov qauv tuaj yeem ncaj qha rau electrophoresis; lub lag luam yog yooj yim thiab yooj yim.

Daim ntawv thov cov khoom siv

Kev nthuav dav ntawm PCR ntawm DNA tawg, npe DNA, DNA sib lawv liag, PCR ntxiv rau tus Tsov tus tw

Cia thiab txee lub neej

Nws tuaj yeem khaws cia ntev ntev ntawm -20°C; rau kev siv ntau zaus, nws tuaj yeem khaws cia ntawm 4°C rau lub sijhawm luv.


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa rau peb