Taw qhia:

RNA molecules tuaj yeem cuam tshuam nrog lwm cov molecules lom, xws li cov protein, DNA thiab RNA, thiab yog li muaj nyob hauv cov hlwb thiab cov kab mob hauv daim ntawv ntawm macromolecular complexes.Ntawm lawv, RNA-protein complexes yog lub neej thiab kev ua haujlwm ntawm RNA Ib qho tseem ceeb ntawm cov ntaub ntawv.RNA rub-down yog ib txoj hauv kev zoo rau kev tshuaj xyuas thiab txheeb xyuas RNA molecules, tshwj xeeb tshaj yog cov protein sib cuam tshuam ntawm lncRNA molecules, thiab tau lees paub los ntawm feem ntau ntawm cov kws tshawb fawb thiab cov ntawv pov thawj kev kawm thoob ntiaj teb.Hnub no FORREGENE yuav qhia rau koj txog kev sim thiab cov txheej txheem ntawm RNA rub-down, tej zaum koj tuaj yeem siv nws

1. Kev sim hauv paus ntsiab lus ntawm RNA rub down technology:

Nyob rau hauv vitro transcription thiab synthesis ntawm biotin-labeled lub hom phiaj lncRNA los yog lncRNA fragments (xws li RNA fragments siv raws li zoo thiab tsis zoo tswj), thiab incubate nrog cell extracts;siv avidin-anchored magnetic hlaws dai los sau RNA-protein lossis RNA-RNA complexes, Tom qab ntxuav tag, lub complex yog eluted.Yog tias kuaj pom cov protein lub hom phiaj molecule uas cuam tshuam nrog lncRNA, ua cov protein electrophoresis ntawm RNA-rub cov khoom, kuaj pom lub hom phiaj protein los ntawm cov nyiaj staining, thiab txiav cov gel rau loj spectrometry;kuj ncaj qha ua western blot verification ntawm cov khoom rub-down;Yog tias kuaj pom Rau RNA lub hom phiaj molecules uas cuam tshuam nrog lncRNA, RNA-rub cov khoom raug raug RNA rho tawm thiab kuaj qRT-PCR tshwj xeeb yog ua.

2. RNA rub down thev naus laus zis kev sim txheej txheem:

3. Kev pabcuam rau RNA rub tawm ntawm FOREGENE:

Cell kab lis kev cai

Kev npaj ntawm cell tag nrho / nuclear / plasma protein extract

RNA rub tawm kev sim

SDS-PAGE electrophoresis, nyiaj stain

Mass spectrometry thiab WB kev sim

Kev sim qhia

4. Cov ntaub ntawv sim

Cov duab hauv qab no qhia txog cov txiaj ntsig ntawm FJ Bio-Bio's RNA rub thiab SDS-PAGE electrophoresis nrhiav pom, uas lub xub liab cim qhov sib txawv band:

Pab pawg tshawb fawb ntawm Xib Fwb Song Xu ntawm Sichuan University tau sau 15 xyoo ntawm kev sib txuas mus ntev hauv kev tshawb fawb lncRNA.Foregene Bio thiab xibfwb Song Xu pawg tshawb fawb tau koom tes los tsim RNA rub down thev naus laus zis ntawm lncRNA, uas yuav ua rau koj txaus siab rau koj qhov kev sim.