• facebook
  • linkedin
  • youtube

1

Hais txog RNA extraction

Peb caug xyoo dhau los, rau RNA rho tawm, qhov peb tuaj yeem xav tsuas yog yuav ua li cas kom tau txais RNA tiav, txawm li cas los xij, tsis muaj qhov yuav tsum tau ua kom nrawm nrawm (Thawj tiam Techology the Trizol txoj kev yug los).Nrog rau qhov hloov tshiab ntawm RNA rho tawm thev naus laus zis, peb tuaj yeem ua dab tsi tsis txwv rau qhov tau txais txiaj ntsig.Thaum ntsib nrog kev rho tawm ntawm thawj tiam Trizol-raws li reagents, cov neeg sim feem ntau muaj teeb meem los ntawm nws cov kauj ruam ua haujlwm cumbersome;Txawm hais tias tom qab ntau qhov kev txhim kho thiab kev tsim kho tshiab, nws tseem siv sijhawm ntau.Nyob rau tib lub sijhawm, cov organic reagents xws li phenol thiab chloroform yuav tsum tau siv rau hauv cov txheej txheem rho tawm, uas tseem cuam tshuam kev nyab xeeb ntawm cov neeg ua haujlwm rau qee yam.

2Ua tau zoo, yooj yim thiab nyab xeeb

Nrhiav kom tau txais txiaj ntsig zoo, yooj yim thiab nyab xeeb RNA cov khoom rho tawm tau dhau los ua lub hom phiaj tseem ceeb ntawm cov neeg sim.Yog li ntawd, Silica Spin Kem adsorption txoj kev rau RNA extraction tau los ua.Foregene thib peb-tiam RNA cov khoom siv RNA cov khoom lag luam, thiab cov ntaub so ntswg zoo nkauj, yog tias tsis muaj kev zam txim rau cov khoom lag luam luv luv, yog li tsis muaj kev sib cav, yog li kom muaj kev pheej hmoo, yog li muaj kev pheej hmoo siab, thiab muaj kev pheej hmoo siab, thiab qis qis ntawm RNA tsis zoo.

Daim ntawv qhia kev ua haujlwm sib piv

3

Cov txiaj ntsig kev sim sib piv daim duab

Foregene tag nrho RNA rho tawm cov khoom muaj siab RNA extraction yield, zoo purity, thiab cov extraction nyhuv ntawm miRNA yog zoo dua li lwm hom khoom siv.

4

Electrophoresis ntawm 50 mg ntawm nas daim siab tag nrho RNA rho tawm los ntawm ntau txoj kev
Thawj tiam (1: Trizol)
Thib ob tiam (2: RNA rho tawm cov khoom siv los ntawm tuam txhab A)
Peb tiam (3: RNA extraction kits plus los ntawm tuam txhab A, 4: Foregene: RNA Isolation kits)

qPCR graph (amplification nkhaus graph)

Genomic DNA yuav tsum raug tshem tawm kom zoo thaum lub sij hawm RNA rho tawm, txwv tsis pub nws yuav muaj kev tswj tsis tau ntawm cov kev sim qis qis (thaum ntxov CT tus nqi, qhov tsis zoo).Cov khoom siv Foregene tom ntej no tuaj yeem tshem tawm cov genomic DNA zoo kawg nkaus yam tsis muaj qhov sib ntxiv ntawm DNase, txuag sijhawm ntau, thaum zam kev degradation los ntawm exogenous RNase paug.
Thawj tiam (ntsuab qhia TRlzol-, liab qhia TRlzol+)

5Thib ob (Xiav qhia Lub Tuam Txhab A RNA cov khoom siv rho tawm -, liab qhia Lub Tuam Txhab A RNA rho tawm cov khoom siv +)

6

Peb tiam (xiav qhia lub tuam txhab A RNA extraction kits plus, ntsuab qhia Foregene RNA Isolation kits)

7

(Ceeb toom: "_" hauv qPCR daim duab qhia tias DNase tsis tau ntxiv, thiab tsis tau tshem tawm cov kab mob DNA; "+" qhia tias DNase tau ntxiv lawm, thiab cov kab mob DNA tau raug tshem tawm)

Ua kom tiav RNA rho tawm hauv 11 min

Tsis xav tau ntau xyoo ntawm nucleic acid extraction kev tsub zuj zuj, Foregene high-efficiency tag nrho RNA cais cov khoom siv tuaj yeem tso cai rau koj kom tau txais cov txiaj ntsig siab thiab siab dawb huv RNA hauv 11 feeb kom tau raws li ntau yam kev sim hauv qab.

9Txog rau tam sim no, Foregene tag nrho RNA isolaton cov khoom siv series cov khoom lag luam tau lees paub los ntawm ntau tus neeg siv khoom thiab tau pab txhawb rau ntau cov ntaub ntawv tau qhab nia siab.Tus neeg siv khoom siv tiag tiag ntawm daim ntawv qhia tawm tswv yim electrophoresis thiab qhov ntau ntawm PCR amplification nkhaus tom qab rov qab sau qhia tias RNA muab rho tawm los ntawm kev siv cov khoom Foregene tsis yog tsuas yog siab siab, tab sis kuj muaj kev ncaj ncees.Tsuas yog cov khoom siv thev naus laus zis uas tau sim los ntawm kev ua lag luam tuaj yeem nyiam los ntawm txhua tus.

1011

Cell Total RNA Isolation Kit

High RNA tawm los

Ceev: Ua kom tiav RNA Kev rho tawm hauv 11 feeb

Kev Nyab Xeeb: Tsis muaj tshuaj lom neeg ntxiv

Qee cov qhab nia siab:

1. Yuan Fang, Zezhong Liu, Yang Qiu, et al.Inhibition of viral suppressor of RNAi proteins by designer peptides protects from enteroviral infection in vivo, Immunity, 54(10), 2021: 2231-2244.e6.(Ib.: 31.745,Viral RNA Isolation KitCat No.RE-02011)

https://www.sciencedirect.com/science/article/pii/S1074761321003617

2. Ren, Y., Wang, A., Wu, D. et al.Dual inhibition ntawm innate tiv thaiv kab mob thiab apoptosis los ntawm tib neeg cytomegalovirus protein UL37x1 ua rau muaj txiaj ntsig zoo dua tus kab mob.Nat Microbiol 7, 1041–1053 (2022).(Ib.: 30.964,Cell Total RNA Isolation KitNr. RE-03111)

https://doi.org/10.1038/s41564-022-01136-6


Post lub sij hawm: Lub Xya hli ntuj-27-2022