Txawm hais tias koj yog ib tus neeg tshiab rau kev lom neeg, lossis tus thawj coj loj uas muaj kev txawj ntse thiab lub zog, koj puas tau thab kom rho tawm cov xov tooj zoo RNA tag nrho?Muaj pes tsawg tus neeg pov tseg cov qauv uas muaj nuj nqis vim yog kev ua haujlwm nyuaj thiab ua haujlwm ntawm cov txheej txheem Trizol?

Tam sim no, txoj moo zoo tab tom los!Ua tiav hais lus zoo rau lub sijhawm siv sijhawm thiab tsis muaj txiaj ntsig ib txwm siv, thiab yooj yim rho tawm tag nrho RNA zoo hauv 16 feeb.

Cell Total RNA Isolation Kit

Lub kaw lus tsis muaj RNase, tsis xav tau chloroform extraction, tsis muaj protein ntau thiab lwm yam impurities, yog li koj tuaj yeem tau txais txiaj ntsig.

Txawm hais tias koj yog tus tshiab tshiab lossis tus thawj coj loj, koj yuav muaj koj tus kheej cov qauv siab rau qhov zoo ntawm RNA rho tawm.

Yog li dab tsi ntawm cov khoom siv yog cov khoom siv zoo tagnrho?

Hauv luv, nws txhais tau tias "ntau dua thiab zoo dua."

Tsis muaj teeb meem, txaus siab rau koj qhov kev ntshaw me ntsis.

Cov khoom siv no tuaj yeem rho tawm tag nrho RNA los ntawm cov hlwb uas muaj nyob hauv 96, 24, 12, thiab 6-zoo daim hlau nrog cov khoom siv siab.Muaj ntau hom RNA rov qab los.Ntxiv rau cov 28s thiab 18s feem ntau, 5s thiab me me ntawm miRNA kuj tseem siv tau.Sau.

Qhov tshwj xeeb DNA-tu kem tso cai rau koj tshem tawm genomic DNA yam tsis tau ntxiv DNase, thiab cov nyhuv zoo dua, ua tau raws li koj qhov kev xav tau ntawm kev sim.

Pom ntawm no, txhua tus tau tsiv mus?Tsis txhob txhawj, cov khoom zoo, tham nrog qhov tseeb!!!

Ib, yooj yim dua!(16 feeb tsis yog hais lus xwb)

二 、 Zoo dua!(Cov nyhuv yog pom)

Foregene Cell Tag Nrho Cov Khoom Siv RNAIsolation Sib piv cov txiaj ntsig ntawm adsorption kem txoj kev thiab Trizol txoj kev

三 、 Ntau ruaj khov!(Ib zaug tau siv nws yuav hais tias nws zoo)

Foregene QPCR tus qauv ntawm cov noob A tom qab hloov pauv

Tswv yim:

Thaum tus nqi ntawm cov hlwb tsawg dhau, tag nrho RNA tawm los yuav raug txo, thiab lub spectrophotometer tsis tuaj yeem txiav txim siab tus nqi OD.Nws raug nquahu kom ua qhov kev xeem zoo los ntawm kev xeem qPCR tom ntej.Cov khoom no ua haujlwm zoo dua nrog peb lub tuam txhab RT EasyTM II (First-strand cDNA synthesis for Real Time PCR) (RT-01022)!

Xaiv Foregene Cultured Cell Tag Nrho RNA Extraction Kit kom koj qhov kev sim ua ib kauj ruam sai dua.Koj tab tom tos dab tsi, ua tam sim no!