Luam tawm los ntawm: CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
Ib: 69.504
Khoom pab:Cell Total RNA Isolation Kit
Original Link:https://www.nature.com/articles/s41586-022-05271-2
Kev kis tus kab mob filoviruses xws li Ebola virus (EBOV) thiab tus kab mob Marburg tuaj yeem ua rau cov tsos mob hnyav, nrog rau mob haemorrhagic kub taub hau thiab ntau lub cev tsis ua haujlwm.4.Ntawm no peb txiav txim siab tus qauv ntawm Ebola tus kab mob L protein nyob rau hauv complex nrog tetrameric VP35 siv cryo-electron microscopy (lub xeev 1).Kev txheeb xyuas cov qauv qhia tau hais tias tus kab mob Ebola L muaj cov kab mob filovirus tshwj xeeb tso rau qhov tseem ceeb rau RNA synthesis, thiab VP35 cuam tshuam nrog thaj tsam N-terminal ntawm L los ntawm peb tus protomers ntawm VP35 tetramer.
Hauv daim ntawv no, tag nrho RNA tau muab rho tawm siv ForegeneCell Total RNA Isolation Kitthiab qhov concentration ntawm RNA tau ntsuas los ntawm Nanodrop ntawm 260 nm, thiab tom qab ntawd ua RT / qPCR nrhiav pom.
■ Tag nrho cov txheej txheem yog ua nyob rau hauv chav tsev kub (15-25 ℃), tsis muaj dej khov da dej thiab tsis kub centrifugation.
■ Cov khoom siv tag nrho yog RNase-Free, tsis tas yuav txhawj txog RNA degradation.
■ DNA-Tshuaj Kem tshwj xeeb khi DNA, kom cov khoom siv tuaj yeem tshem tawm cov kab mob DNA sib kis tsis tau ntxiv DNase ntxiv.
■ High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv huv RNA zoo.
■ Ceev ceev: yooj yim rau kev khiav lag luam thiab tuaj yeem ua tiav hauv 11 feeb.
■ Kev Nyab Xeeb: Tsis tas yuav tsum muaj cov organic reagent.
■ Zoo: RNA purified yog purity siab, tsis muaj protein ntau thiab lwm yam impurities, thiab tuaj yeem ua tau raws li ntau yam kev sim tom qab.
Post lub sij hawm: Nov-04-2022
